![Immunohistochemistry: IgG Heavy Chain Antibody (RM116) [Unconjugated] [NBP2-62019] - Human Tonsil using NBP2-62019.](http://images.novusbio.com/fullsize/IgG-Heavy-Chain-Antibody-RM116-Unconjugated-Immunohistochemistry-NBP2-62019-img0001.jpg "Immunohistochemistry: IgG Heavy Chain Antibody (RM116) [Unconjugated] [NBP2-62019] - Human Tonsil using NBP2-62019.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ICC/IF, IHC, ELISA |
| Clone | RM116 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-62019B | |||
| Unconjugated | NBP3-25964 | |||
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Human IgG |
| Specificity | This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including gamma 1, gamma 2, gamma 3, and gamma 4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for IgG Heavy Chain Antibody (NBP2-62019)Find related products by research area.
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![Sandwich ELISA Detection: IgG Heavy Chain Antibody (RM116) [NBP2-62019] - Sandwich ELISA using NBP2-62019 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.](http://images.novusbio.com/fullsize/IgG-Heavy-Chain-Antibody-RM116-Unconjugated-ELISA-NBP2-62019-img0008.jpg "Sandwich ELISA Detection: IgG Heavy Chain Antibody (RM116) [NBP2-62019] - Sandwich ELISA using NBP2-62019 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.")
![Sandwich ELISA Capture: IgG Heavy Chain Antibody (RM116) [NBP2-62019] - Sandwich ELISA using NBP2-62019 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.](http://images.novusbio.com/fullsize/nbp2-62019_rabbit-monoclonal-igg-heavy-chain-antibody-rm116-131020231935491.png "Sandwich ELISA Capture: IgG Heavy Chain Antibody (RM116) [NBP2-62019] - Sandwich ELISA using NBP2-62019 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.")
![ELISA: IgG Heavy Chain Antibody (RM116) [NBP2-62019] - ELISA of human immunoglobulins shows RM116 reacts to the gamma 1, gamma 2, gamma 3, gamma 4 heavy chains of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62019_rabbit-monoclonal-igg-heavy-chain-antibody-rm116-17720241003242.png "ELISA: IgG Heavy Chain Antibody (RM116) [NBP2-62019] - ELISA of human immunoglobulins shows RM116 reacts to the gamma 1, gamma 2, gamma 3, gamma 4 heavy chains of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![ELISA: IgG Heavy Chain Antibody (RM116) [NBP2-62019] - A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62019_rabbit-monoclonal-igg-heavy-chain-antibody-rm116-1772024936261.png "ELISA: IgG Heavy Chain Antibody (RM116) [NBP2-62019] - A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")