Horse anti-Mouse IgG ImmPRESS™ Secondary Antibody [HRP Polymer]

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Small Bowel: Cytokeratin 8/18 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown), Hematoxylin QS (blue).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Rhesus macaque formalin/paraffin lymph node: CD4 (1F6), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB substrate (brown). Hematoxylin counterstain (blue). Courtesy of Charles Brown, NIH, NIAID, Laboratory of Molecular Microbiology.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Tonsil: CD8 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin QS counterstain (blue).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Tonsil: Bcl-2 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin QS counterstain (blue).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Lymphoma: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40) , ImmPress anti-mouse Ig reagent (MP-7402), and Vector NovaRED peroxidase substrate (red). Hematoxylin QS counterstain (blue)

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Tonsil: CD20 (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT VIP (purple) substrate

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPress anti-mouse Ig reagent (MP-7402), and Vector NovaRED peroxidase substrate (red). Hematoxylin QS counterstain

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon: PGP 9.5 (m, VP-P983), ImmPRESS Anti-Mouse Ig Kit, ImmPACT SG (blue-gray) substrate.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Kidney: PGP 9.5 (m, VP-P983), ImmPRESS Anti-Mouse Ig Kit, ImmPACT NovaRED (red) substrate. Hematoxylin (blue) counterstain.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon: Peripherin (m, VP-P968), ImmPRESS Anti-Mouse Ig Kit, ImmPACT AEC (red) substrate.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon: PGP 9.5 (m, VP-P983), ImmPRESS Anti-Mouse Ig Kit, ImmPACT SG (blue-gray) substrate.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon: PGP 9.5 (m, VP-P983), ImmPRESS Anti-Mouse Ig Kit, ImmPACT AEC (red) substrate. Hematoxylin QS (blue) counterstain.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Breast: Estrogen Receptor (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin counterstain (blue).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPress anti-mouse Ig reagent, and Vector NovaRED peroxidase substrate ( red). Hematoxylin QS counterstain

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Small Bowel: Peripherin (m), ImmPRESS Anti-Mouse Ig Kit, Vector NovaRED Substrate Kit (red). Hematoxylin QS Counterstain (blue).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector VIP substrate (purple); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector SG substrate (blue/gray).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon: Peripherin (m, VP-P968), ImmPRESS Anti-Mouse Ig Kit, ImmPACT AEC (red) substrate.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Lymphoma: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40), ImmPress anti-mouse Ig reagent, and Vector NovaRED peroxidase substrate (red). Hematoxylin QS counterstain (blue)

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Breast: CD34 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin counterstain (blue).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon: Peripherin (m, VP-P968), ImmPRESS Anti-Mouse Ig Kit, ImmPACT NovaRED (red) substrate. Hematoxylin QS (blue) counterstain.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Prostate: Smooth muscle actin (m), ImmPRESS Anti-Mouse Ig Kit, AEC Substrate Kit (red). Hematoxylin QS counterstain (blue). Mounted in VectaMount AQ Mounting Medium.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Small Intestine (double label): ImmPRESS Anti-Mouse Ig using CD10 primary antibody with DAB/Ni (black) substrate, and ImmPRESS Anti-Mouse Ig using Cytokeratin 20 primary antibody with Vector NovaRED (red) substrate. No counterstain.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Small Bowel (double label): Peripherin (m), ImmPRESS Anti-Mouse Ig Kit, Vector NovaRED (red) substrate. Desmin (m), ImmPRESS Anti-Mouse Ig Kit, DAB/Ni (black) substrate.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Colon: Peripherin (m, VP-P968), ImmPRESS Anti-Mouse Ig Kit, ImmPACT SG (blue-gray) substrate.

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector NovaRED substrate (red); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector DAB+Ni substrate (gray/black).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Small Bowel: CD10 (m), ImmPRESS Anti-Mouse Ig Kit, DAB Substrate Kit (brown). Hematoxylin QS counterstain (blue).

Immunohistochemistry: anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Rhesus macaque formalin/paraffin lymph node: CD4 (1F6), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB substrate (brown). Hematoxylin counterstain (blue). Courtesy of Charles Brown, NIH, NIAID, Laboratory of Molecular Microbiology.

anti-Mouse IgG ImmPRESS(TM) Secondary Antibody [MP-7402-NB] - Tonsil: Cytokeratin (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT DAB (brown) substrate. Hematoxylin (blue) counterstain.

• Reactivity: Mu
• Applications: ELISA, Func, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Horse
• Conjugate: HRP Polymer

Horse anti-Mouse IgG ImmPRESS™ Secondary Antibody [HRP Polymer] Summary

• Description: Features:
• High sensitivity
• Low background
• One-step detection
• Micropolymer technology
• Enhanced accessibility to nuclear and membrane antigens
• Ready-to-use in a convenient dropper bottle
• Shorter assay time
• Simplified multiple labeling
• Immunogen: Mouse IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Horse
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: No Preservative
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The ImmPRESS(TM) polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.
The peroxidase micropolymers of the ImmPRESS(TM) HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.