Recombinant Human IkB-alpha His Protein

Recombinant Human IkB-alpha Protein [NBP1-72500]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human IkB-alpha His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-317 of Human IkB-alpha
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN YNGHTCLHLA SIHGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE DELPYDDCVF GGQRLTL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NFKBIA
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 37.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human IkB-alpha His Protein

• alpha
• I-kappa-B-alpha
• IkappaBalphaNF-kappa-B inhibitor alpha
• IKBAikB-alpha
• IkBalpha
• IkB-alpha
• MAD3
• MAD-3
• Major histocompatibility complex enhancer-binding protein MAD3
• NFKBI
• NFKBIA
• nuclear factor of kappa light chain gene enhancer in B-cells
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kappa-B inhibitor alpha, also known as NFKBIA, is members of the IkB family of proteins can be divided into four groups (IkB-alpha, IkB-beta, IkB-gamma, IkB-epsilon). NFKBIA inhibits the NFkB complex by binding and sequestering it in the cytoplasm. Upon stimulation, NFKBIA is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Recombinant human NFKBIA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NFKBIA