IKK gamma Antibody (46B844)

Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: IKK gamma Antibody (46B844) [NB100-56532] - Human Jurkat cells were probed using 0.1 ug of NEMO antibody (red) and 0.1 ug of isotype control (green), with shaded histogram representing cells alone and an anti-mouse IgG-FITC secondary antibody.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: 46B844
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IKK gamma Antibody (46B844) Summary

• Immunogen: Full-length human IKK gamma protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IKBKG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Western Blot 2 ug/ml
• Application Notes: A 48 kDa band is observed.
• Antagonist:
  • IKK gamma Inhibitor Peptide Set NBP2-26504

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IKK gamma Antibody (46B844)

• FIP-3IP2
• FIP3NF-kappa-B essential modifier
• FIP3P
• I-kappa-B kinase subunit gamma
• IkB kinase gamma subunit
• IkB kinase subunit gamma
• IkB kinase-associated protein 1
• IKBKG
• IKK gamma
• IKKAP1
• IKKG
• IKK-gammaIPD2
• incontinentia pigmenti
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Inhibitor of nuclear factor kappa-B kinase subunit gamma
• IP
• IP1
• NEMO
• NEMOAMCBX1
• NFkappaB essential modulator
• NF-kappa-B essential modulator

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK3/g preferentially interacts with IKK2/b and is required for activation of IKK complex. IKK3/g is also known as NEMO (NF-kB Essential MOdulator). Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKa nor IKKb, but complexes directly with IKKg. This suggests that IKKg may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IKK gamma Antibody (NB100-56532)(5)

Publications using NB100-56532

• Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway Mol Pharmacol 2010-05-01 [PMID: 20103608] (WB)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Miller BS, Zandi E. Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation. J Biol Chem. 2001-09-28 [PMID: 11470787]
• Xiao G, Harhaj EW, Sun SC. Domain-specific interaction with the I kappa B kinase (IKK)regulatory subunit IKK gamma is an essential step in tax-mediated activation of IKK. J Biol Chem. 2000-11-03 [PMID: 10906125]
• Harhaj EW, Good L, Xiao G et al. Somatic mutagenesis studies of NF-kappa B signaling in human T cells: evidence for an essential role of IKK gamma in NF-kappa B activation by T-cell costimulatory signals and HTLV-I Tax protein. Oncogene. 2000-03-09 [PMID: 10723136]
• Harhaj EW, Sun SC. IKKgamma serves as a docking subunit of the IkappaB kinase (IKK) and mediates interaction of IKK with the human T-cell leukemia virus Tax protein. J Biol Chem. 1999-08-13 [PMID: 10438454]

Bioinformatics

• Gene Symbol: IKBKG
• Entrez:
  • 8517(Human)
• Uniprot:
  • NP_001093326(Human)