IL-1 RI Antibody (L1-31-22.2.1) - Azide and BSA Free

Immunohistochemistry-Paraffin: IL-1 RI Antibody (40101) [NBP1-50304] - Analysis of anti-IL1R1 antibody with sheep tonsil. Image from verified customer review.

Immunohistochemistry-Paraffin: IL-1 RI Antibody (40101) [NBP1-50304] - Analysis of anti-IL1R1 antibody with human placenta at concentration 10 ug/ml.

Product Discontinued

IL-1 RI Antibody (L1-31-22.2.1) - Azide and BSA Free Summary

• Immunogen: The extracellular domain of the IL-1 RI.
• Specificity: These antibodies react with cell surface and soluble human IL-1 receptor; Specific epitopes are unknown, but they do partially inhibit (30-50%) binding of one another to IL-1 receptor. They do not block binding of IL-1 to receptor, and they do not cross-react with receptors for human IL-2 or IL-4.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1R1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Radioimmunoassay 1:100 - 1:2000

Reactivity Notes

Sheep reactivity reported from a verified customer review. Bovine reactivity reported in (PMID: 31412964).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) Sourced in Ascites.
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for IL-1 RI Antibody (L1-31-22.2.1) - Azide and BSA Free

• CD121 antigen-like family member A
• CD121a antigen
• CD121a
• IL-1 RI
• IL1R
• IL1R1
• IL1RI
• IL-1RI
• IL1RT1
• IL-1RT1
• IL-1RT-1
• Interleukin 1 Receptor 1
• interleukin 1 receptor, type I
• interleukin receptor 1
• interleukin-1 receptor type 1
• p80

Background

The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family (1). IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway (2-3). Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1 (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 RI Antibody (NBP1-50304)(3)

Publications using NBP1-50304

• Peralta M, Cainelli S, Stassi A et al. Endometrial expression of members of the IL-1 family: their involvement in delayed conception of dairy cows Theriogenology 2023-01-01 [PMID: 36335720] (IF/IHC, Bovine)
• Stassi AF, Baravalle ME, Belotti EM et al. Altered expression of IL-1beta, IL-1RI, IL-1RII, IL-1RA and IL-4 could contribute to anovulation and follicular persistence in cattle Theriogenology [PMID: 31412964] (WB, IHC-P, Bovine)
• Stassi AF, Gareis NC, Marelli BE et al. Follicular structures of cows with cystic ovarian disease present altered expression of cytokines Zygote [PMID: 29334661] (IF/IHC, Bovine)

Review for IL-1 RI Antibody (NBP1-50304) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer IHC-P Other 08/18/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: sheep tonsil
• Species: Other

Bioinformatics

• Gene Symbol: IL1R1
• Entrez:
  • 3554(Human)
• Uniprot:
  • P14778()