Human IL-12 p70/IL-12A ELISA Kit

ELISA: IL-12 p70/IL-12A ELISA kit [NBP3-11729] - Representative standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell culture supernatants
• Standard Curve Range: 3.16 - 1000 pg/mL
• Sensitivity: 1 pg/mL

Human IL-12 p70/IL-12A ELISA Kit Summary

• Standard Curve Range: 3.16 - 1000 pg/mL
• Sensitivity: 1 pg/mL
• Inter-Assay: %CV < 11.2
• Intra-Assay: %CV < 6.7
• Spike Recovery: 94 - 107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit
• Gene: IL12A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-12 p70/IL-12A ELISA Kit

• CLMF p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL12 p70
• IL-12 p70
• IL-12 subunit p35
• IL-12, subunit p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin-12, or IL-12, also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine that affects both natural killer (NK) cells and T lymphocytes. IL-12 is a heterodimer of subunit IL-12A p35 and subunit IL-12B p40 that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. It is involved in mediating many aspects of antiviral immunity. In addition to stimulating T helper cell development, IL-12 is a potent inducer of interferon-gamma (IFN-gamma) production by T cells and NK cells. Increased IL-12 production is a consequence of EBV, CMV, LCMV or HSV infection. While it is unclear what separate effects can be attributed to each IL-12 subunit, evidence suggests IL-12A p35 is the biologically active component responsible for signal transduction via the IL-12 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL12A