IL-27R alpha/WSX-1/TCCR Antibody - BSA Free

Western Blot: IL-27R alpha/WSX-1/TCCR Antibody [NBP1-76728] - Analysis of IL27RA expression in human spleen tissue lysates with anti-IL27RA using NBP1-76728 at 1 ug /ml.

Immunocytochemistry/ Immunofluorescence: IL-27R alpha/WSX-1/TCCR Antibody - BSA Free [NBP1-76728] - Immunofluorescence of IL-27R alpha/WSX-1/TCCR in A549 cells with IL-27R alpha/WSX-1/TCCR antibody at 20 u/mL.

Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody - BSA Free [NBP1-76728] - Immunohistochemistry of IL-27R alpha/WSX-1/TCCR in human spleen tissue with IL-27R alpha/WSX-1/TCCR antibody at 10 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL-27R alpha/WSX-1/TCCR Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human TCCR precursor. The immunogen is located within the first 50 amino acids of TCCR. Amino Acid Squence: GSAGPLQCYGVGPLGD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL27RA
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: An approximately 70 kDa band can be detected in Western Blot.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IL-27R alpha/WSX-1/TCCR Protein (NBP1-76728PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IL-27R alpha/WSX-1/TCCR Antibody - BSA Free

• class I cytokine receptor
• CRL1IL-27R
• Cytokine receptor-like 1
• IL-27 R alpha
• IL27R alpha
• IL27R
• IL27RA
• IL-27Ra
• IL-27R-alpha
• interleukin 27 receptor, alpha
• interleukin-27 receptor subunit alpha
• TCCR
• TCCRIL-27 receptor subunit alpha
• T-cell cytokine receptor type 1
• Type I T-cell cytokine receptor
• WSX-1
• WSX1IL-27R subunit alpha
• zcytor1

Background

Upon antigen challenge, T-helper cells differentiate into two functional distinct subsets, Th1 and Th2. Th1 cells produce IL-2, IFN-gamma and lymphotoxin-beta that augment cell mediated immune response while Th2 cells secrete IL-4, IL-5, and IL-10 that enhance humoral immunity. The function of T-helper cells is regulated by cytokines. A novel cytokine receptor was recently identified and cloned. It is a new member in the type I cytokine receptor family and designated TCCR for T-cell cytokine receptor and WSX-1. TCCR deficient mice had impaired Th1 responses to protein antigen challenge, including decreased levels of IFN-gamma and Th1-dependent antibody IgG2a . TCCR is predominately expressed in thymus, spleen, lymph notes and peripheral blood leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-27R alpha/WSX-1/TCCR Antibody (NBP1-76728)(2)

Publications using NBP1-76728

• Di Carlo Emma, Sorrentino Carlo, Zorzoli Alessia et al. The antitumor potential of Interleukin-27 in prostate cancer. Oncotarget 2014-01-01 [PMID: 24681516] (WB, Human)
• Morandi F, Di Carlo E, Ferrone S et al. IL-27 in Human Secondary Lymphoid Organs Attracts Myeloid Dendritic Cells and Impairs HLA Class I-Restricted Antigen Presentation. J. Immunol. 2014-02-20 [PMID: 24554774] (IHC-P, Human)

FAQs for IL-27R alpha/WSX-1/TCCR Antibody (NBP1-76728). (Showing 1 - 1 of 1 FAQs).

1. We need to know the sequence of the immunogen peptide. Are you able to supply me with this information?
   • The peptide sequence is within amino acids 11-60 of NP_004834.1.

Bioinformatics

• Gene Symbol: IL27RA
• Uniprot:
  • NP_004834()