IL-33 Antibody (1C5)

Western Blot: IL-33 Antibody (1C5) [NBP2-31145] - Analysis of IL33 in (1) U2OS and (2) HUVEC cell lysates.

Immunohistochemistry-Paraffin: IL-33 Antibody (1C5) [NBP2-31145] - Analysis of IL33 protein in normal human spleen tissue sections using IL33 antibody (clone 1C5) at a concentration of 5 ug/ml. This antibody stained the nuclei as well as the cytoplasm of granulocytes and some other cell types in the red pulp of spleen tissue.

Western Blot: IL-33 Antibody (1C5) [NBP2-31145] - Analysis of IL33 recombinant protein using IL33 antibody (clone 1C5) at a concentration of 2 ug/ml.

Product Discontinued

IL-33 Antibody (1C5) Summary

• Immunogen: Synthetic peptide corresponding to an internal sequence of the human IL33 protein (between amino acids 100-270) [UniProt# O95760]
• Localization: Nucleus, Cytoplasm, Secreted
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL33
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: The canonical form of IL33 i.e. Isoform 1 is 270 amino acids long chain with predicted molecular weight of 30.8 kDa, however, this full length form gets processed via cleavage of n-terminal 111 amino acids (pro-peptide cleavage) to generate 159 amino acids long mature IL33 protein. In WB, the full length protein runs at ~30kDa, whereas, the processed mature form runs at ~18kDa position.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for IL-33 Antibody (1C5)

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL33
• Entrez:
  • 90865(Human)
• Uniprot:
  • O95760()