IL-34 Antibody (578416)

Recombinant Human IL‑34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑34 Monoclonal Antibody (Catalog # MAB5265). The ND50 is typically 0.01-0.05 µg/mL.

• Reactivity: Hu
• Applications: B/N, CyTOF-ready, Flow
• Clone: 578416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-34 Antibody (578416) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-34
  Asn21-Pro242
  Accession # Q6ZMJ4
• Specificity: Detects recombinant human IL-34 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-34 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL34
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <1.0 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.01-0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-34 Antibody (578416)

• C16orf77
• IL34
• IL-34
• interleukin 34

Background

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

1. Lin, H. et al. (2008) Science 320:807.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-34 Antibody (MAB5265)(1)

Publication using MAB5265

• Bezie S, Picarda E, Ossart J, Tesson L, Usal C, Renaudin K, Anegon I, Guillonneau C IL-34 is a Treg-specific cytokine and mediates transplant tolerance. J Clin Invest, 2015-09-21;125(10):3952-64. 2015-09-21 [PMID: 26389674] (Neutralization, Human, Rat)

Bioinformatics

• Gene Symbol: IL34
• Uniprot:
  • Q6ZMJ4(Human)