IL-3R alpha/CD123 Antibody (IL3RA/2947R) - Azide and BSA Free

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Immunohistochemistry-Paraffin: IL-3R alpha/CD123 Antibody (IL3RA/2947R) - Azide and BSA Free [NBP2-79851] - Formalin-fixed, paraffin-embedded human Tonsil stained with IL-3R alpha/CD123 Antibody (IL3RA/2947R).
Protein Array: IL-3R alpha/CD123 Antibody (IL3RA/2947R) - Azide and BSA Free [NBP2-79851] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IL-3R alpha/CD123 Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, MA
Clone
IL3RA/2947R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

IL-3R alpha/CD123 Antibody (IL3RA/2947R) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79717).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment of human IL-3R alpha/CD123 protein (around aa 26-171) (exact sequence is proprietary) (Uniprot: P26951)
Localization
Cell Surface, Cytoplasm
Marker
Acute Myeloid Leukemia Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IL3RA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 0.25-0.5 ug/ml
  • Immunohistochemistry-Paraffin 0.25-0.5 ug/ml
  • Protein Array
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-79851.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for IL-3R alpha/CD123 Antibody (IL3RA/2947R) - Azide and BSA Free

  • CD123 antigen
  • CD123
  • hIL3Ra
  • hIL-3Ra
  • IL-3 R alpha
  • IL-3 receptor subunit alpha
  • IL3R alpha
  • IL-3R alpha
  • IL-3R subunit alpha
  • IL3R
  • IL3RA
  • IL-3Ra
  • IL-3R-alpha
  • IL3RAY
  • IL3RX
  • IL3RY
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin-3 receptor subunit alpha
  • MGC34174

Background

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-3R alpha/CD123 Antibody (NBP2-79851)(1)

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Bioinformatics

Gene Symbol IL3RA