IL-3R alpha/CD123 Antibody - BSA Free

Western Blot: IL-3 R alpha/CD123 Antibody [NBP2-16953] - IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein by western blot analysis. THP-1 whole cell extracts (30 ug), non-transfected (-) and IL3 Receptor alpha-transfected (+) 293T whole cell extracts (5 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL3 Receptor alpha antibody diluted by 1:1000

Immunohistochemistry-Paraffin: IL-3 R alpha/CD123 Antibody [NBP2-16953] - Human esophageal cancer. IL3 Receptor alpha antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: IL-3 R alpha/CD123 Antibody [NBP2-16953] - Human breast cancer, using IL3 Receptor alpha antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IL-3R alpha/CD123 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human IL-3R alpha/CD123. The exact sequence is proprietary.
• Localization: Membrane; Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL3RA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for IL-3R alpha/CD123 Antibody - BSA Free

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL3RA
• Uniprot:
  • P26951()