IL-6 Antibody (1936) [Unconjugated]

IL‑6 was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human peripheral blood mononuclear cells (PBMCs) treated with 100 ng/mL LPS for 24 hours were stained with Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2061, filled histogram) or isotype control antibody (MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061). The ND50 is typically 0.05-0.15 µg/mL.

IL‑6 was detected in immersion fixed Human PBMCs stimulated with LPS (positive control) and absent in untreated human PBMCs (negative control) using Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

PBMCs stimulated with 100 ng/ml LPS and Brefeldin A for 24 hrs (A) or unstimulated (B) were stained with Mouse Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB2061) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: IHC, B/N, CyTOF-ready, Flow, ICC/IF
• Clone: 1936
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-6 Antibody (1936) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL‑6
  Val30-Met212
  Accession # P05231
• Specificity: Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.20 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 3-15 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 8.00-80.0 ng/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6 Antibody (1936) [Unconjugated]

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6 Antibody (MAB2061)(50)

Publications using MAB2061

• Wang, S;Xu, Q;Zhang, Y;Jiang, X;Wang, N;Hu, Y;Lu, Y;Wang, Y;Shao, F;Cao, H; The FGF23-Klotho axis promotes microinflammation in chronic kidney disease Cytokine 2024-10-24 [PMID: 39454251] (Immunohistochemistry, Human)
• Chan, ASL;Zhu, H;Narita, M;Cassidy, LD;Young, ARJ;Bermejo-Rodriguez, C;Janowska, AT;Chen, HC;Gough, S;Oshimori, N;Zender, L;Aitken, SJ;Hoare, M;Narita, M; Titration of RAS alters senescent state and influences tumour initiation Nature 2024-08-07 [PMID: 39112713] (Western Blot, Mouse)
• TJ Meyer, S Hackenberg, M Herrmann, T Gehrke, M Steber, R Hagen, N Kleinsasse, A Scherzad Head and neck squamous cancer cells enhance the differentiation of human mesenchymal stem cells to adipogenic and osteogenic linages in vitro Oncology Letters, 2022-10-31;24(6):450. 2022-10-31 [PMID: 36420071] (Neutralization, Human)
• A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system Nature Communications, 2022-10-14;13(1):6078. 2022-10-14 [PMID: 36241617] (Neutralization, Human)
• Hoai-Nga Thi Nguyen, Marie Kawahara, Cat-Khanh Vuong, Mizuho Fukushige, Toshiharu Yamashita, Osamu Ohneda SARS-CoV-2 M Protein Facilitates Malignant Transformation of Breast Cancer Cells Frontiers in Oncology 6/7/2022 [PMID: 35747796]
• S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human)
• Somasundaram A, Cillo A, Lampenfeld C, Workman C, Kunning S, Oliveri L, Velez M, Joyce S, Calderon M, Dadey R, Rajasundaram D, Normolle D, Watkins S, Herman J, Kirkwood J, Lipson E, Ferris R, Bruno T, Vignali D Systemic Immune Dysfunction in Cancer Patients Driven by IL6 Induction of LAG3 in Peripheral CD8+ T Cells. Cancer Immunol Res, 2022-07-01;10(7):885-899. 2022-07-01 [PMID: 35587532] (Neutralization, Human)
• Karavyraki M, Porter RK. Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma Medical Oncology 2022-04-29 [PMID: 35484352]
• Karavyraki M, Porter RK. Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma Medical Oncology 4/29/2022 [PMID: 35484352]
• Y Zhu, LGPL Prata, EOW Gerdes, JME Netto, T Pirtskhala, N Giorgadze, U Tripathi, CL Inman, KO Johnson, A Xue, AK Palmer, T Chen, K Schaefer, JN Justice, AM Nambiar, N Musi, SB Kritchevsk, J Chen, S Khosla, D Jurk, MJ Schafer, T Tchkonia, JL Kirkland Orally-active, clinically-translatable senolytics restore alpha-Klotho in mice and humans EBioMedicine, 2022-03-13;0(0):103912. 2022-03-13 [PMID: 35292270] (Neutralization, Human)
• J Whitburn, SR Rao, EV Morris, S Tabata, A Hirayama, T Soga, JR Edwards, Z Kaya, C Palmer, FC Hamdy, CM Edwards Metabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survival Science Advances, 2022-02-25;8(8):eabf9096. 2022-02-25 [PMID: 35213227] (Neutralization, Human)
• Kuo‐Chu Lai, Zi‐Xuan Hong, Jyh‐Gang Hsieh, Hui‐Ju Lee, Muh‐Hwa Yang, Chia‐Husu Hsieh et al. IFIT2‐depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice Journal of Cachexia, Sarcopenia and Muscle 4/1/2022 [PMID: 35170238]
• Christopher J. Neufeldt, Berati Cerikan, Mirko Cortese, Jamie Frankish, Ji-Young Lee, Agnieszka Plociennikowska et al. SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-kappa B Communications Biology 1/12/2022 [PMID: 35022513]
• T Fukasawa, A Yoshizaki, S Ebata, A Yoshizaki-, Y Asano, A Enomoto, K Miyagawa, Y Kazoe, K Mawatari, T Kitamori, S Sato Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model Elife, 2021-12-02;10(0):. 2021-12-02 [PMID: 34854378] (Neutralization, Human)
• Cynthia Hajal, Yoojin Shin, Leanne Li, Jean Carlos Serrano, Tyler Jacks, Roger D. Kamm The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain Science Advances 6/25/2021 [PMID: 34162553]
• R Emig, W Knodt, MJ Krussig, CM Zgierski-J, O Gorka, O Gro beta , P Kohl, U Ravens, R Peyronnet Piezo1 Channels Contribute to the Regulation of Human Atrial Fibroblast Mechanical Properties and Matrix Stiffness Sensing Cells, 2021-03-16;10(3):. 2021-03-16 [PMID: 33809739] (Neutralization, Human)
• S Gonçalves, K Yin, Y Ito, A Chan, I Olan, S Gough, L Cassidy, E Serrao, S Smith, A Young, M Narita, M Hoare COX2 regulates senescence secretome composition and senescence surveillance through PGE2 Cell Reports, 2021-03-16;34(11):108860. 2021-03-16 [PMID: 33730589] (ICC, Western Blot, Human)
• Maureen Van de Velde, Marie Ebroin, Tania Durré, Marc Joiret, Lionel Gillot, Silvia Blacher et al. Tumor exposed-lymphatic endothelial cells promote primary tumor growth via IL6 Cancer Letters 1/28/2021 [PMID: 33080310]
• Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer Journal of Experimental Medicine 8/3/2020 [PMID: 32434218]
• Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer Journal of Experimental Medicine 2020-08-03 [PMID: 32434218] (Neutralization, Human)
• Kun-Chun Chiang, Kang-Shuo Chang, Shu-Yuan Hsu, Hsin-Ching Sung, Tsui-Hsia Feng, Mei Chao et al. Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells Antioxidants (Basel) 3/19/2020 [PMID: 32204510]
• Davorka Herman Herman Mahečić, Maja Cigrovski Cigrovski Berković, Vanja Zjačić-Rotkvić, Tamara Čačev, Sanja Kapitanović, Monika Ulamec Inflammation-related cytokines and their roles in gastroenteropancreatic neuroendocrine neoplasms Bosnian Journal of Basic Medical Sciences 11/2/2020 [PMID: 32156252]
• LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4652-4660. 2019-11-01 [PMID: 31743940] (Neutralization, Human)
• R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 2019-11-01;10(1):5016. 2019-11-01 [PMID: 31676788] (Neutralization, Human)
• H Huang, Y Zhang, V Gallegos, N Sorrelle, MM Zaid, J Toombs, W Du, S Wright, M Hagopian, Z Wang, AN Hosein, AA Sathe, C Xing, EJ Koay, KE Driscoll, RA Brekken Targeting TGFbetaR2-mutant tumors exposes vulnerabilities to stromal TGFbeta blockade in pancreatic cancer EMBO Mol Med, 2019-10-14;0(0):e10515. 2019-10-14 [PMID: 31609088] (Western Blot, Human)
• MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells Sci Rep, 2019-07-10;9(1):10007. 2019-07-10 [PMID: 31292483] (Bioassay, Human)
• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019-06-11;5(0):. 2019-06-11 [PMID: 31184599] (IHC, Human)
• N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer Oncogene, 2018-04-26;37(29):4013-4032. 2018-04-26 [PMID: 29695834] (Neutralization, Human)
• MA Hjort, H Hov, P Abdollahi, EN Vandsemb, UM Fagerli, B Lund, TS Slørdahl, M Børset, TB Rø Phosphatase of regenerating liver-3 (PRL-3) is overexpressed in classical Hodgkin lymphoma and promotes survival and migration Exp Hematol Oncol, 2018-04-10;7(0):8. 2018-04-10 [PMID: 29651360] (Neutralization, Human)
• Y Su, P Wang, H Shen, Z Sun, C Xu, G Li, T Tong, J Chen Protein kinase D1-mediated classical protein secretory pathway regulates oncogene Ras-induced senescent response J. Cell. Sci., 2018-03-16;0(0):. 3/16/2018 [PMID: 29420297]
• R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum Oral fibroblasts modulate the macrophage response to bacterial challenge Sci Rep, 2017-09-14;7(1):11516. 2017-09-14 [PMID: 28912533] (Neutralization, Human)
• E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma PLoS ONE, 2017-04-14;12(4):e0175712. 2017-04-14 [PMID: 28410380] (Neutralization, Human)
• Shereen Oon, Huy Huynh, Tsin Yee Tai, Milica Ng, Katherine Monaghan, Mark Biondo et al. A cytotoxic anti-IL-3R alpha antibody targets key cells and cytokines implicated in systemic lupus erythematosus JCI Insight 10/5/2016 [PMID: 27699260]
• M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita NOTCH1 mediates a switch between two distinct secretomes during senescence Nat. Cell Biol, 2016-08-15;18(9):979-92. 2016-08-15 [PMID: 27525720] (Western Blot, Human)
• Wan-Chien Cheng Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses Eur J Immunol, 2016-08-01;0(0):. 2016-08-01 [PMID: 27334899] (Neutralization, Human)
• Toral P. Kobawala, Trupti I. Trivedi, Kinjal K. Gajjar, Darshita H. Patel, Girish H. Patel, Nandita R. Ghosh Significance of Interleukin-6 in Papillary Thyroid Carcinoma Journal of Thyroid Research 3/13/2016 [PMID: 27034885]
• Mahito Sadaie, Christian Dillon, Masako Narita, Masashi Narita, Andrew R J Young, Claire J Cairney et al. Cell-based screen for altered nuclear phenotypes reveals senescence progression in polyploid cells after Aurora kinase B inhibition Molecular Biology of the Cell 9/1/2015 [PMID: 26133385]
• Tiina Ilves, Ilkka T Harvima Decrease in Chymase Activity is Associated with Increase in IL-6 Expression in Mast Cells in Atopic Dermatitis Acta Dermato Venereologica 1/1/2015 [PMID: 25270893]
• Suwara M, Green N, Borthwick L, Mann J, Mayer-Barber K, Barron L, Corris P, Farrow S, Wynn T, Fisher A, Mann D IL-1alpha released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts. Mucosal Immunol, 2013-10-30;7(3):684-93. 2013-10-30 [PMID: 24172847] (ICC, Human)
• Becker , T M, Boyd , S C, Mijatov , B, Gowrishankar , K, Snoyman , S, Pupo , G M, Scolyer , R A, Mann , G J, Kefford , R F, Zhang , X D, Rizos , H Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor. Oncogene, 2013-03-04;33(9):1158-66. 2013-03-04 [PMID: 23455323] (Neutralization, Human)
• Yuling Wang, Mohammad Salehi, Max Schütz, Katharina Rudi, Sebastian Schlücker Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells The Analyst 3/21/2013 [PMID: 23364184]
• Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam. Mod Pathol, 2012-11-23;26(3):357-69. 2012-11-23 [PMID: 23174938] (IHC-P, Human)
• Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 2012-09-25;10(9):e1001395. 2012-09-25 [PMID: 23055831] (Neutralization, Human)
• Steinbicker AU, Sachidanandan C, Vonner AJ Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation. Blood, 2011-03-10;117(18):4915-23. 2011-03-10 [PMID: 21393479] (Western Blot, Human)
• Charlotta J Olsen, José Moreira, Eugene M Lukanidin, Noona S Ambartsumian Human mammary fibroblasts stimulate invasion of breast cancer cells in a three-dimensional culture and increase stroma development in mouse xenografts BMC Cancer 12/1/2010 [PMID: 20723242]
• Charlotta J Olsen, José Moreira, Eugene M Lukanidin, Noona S Ambartsumian Human mammary fibroblasts stimulate invasion of breast cancer cells in a three-dimensional culture and increase stroma development in mouse xenografts BMC Cancer 2010-12-01 [PMID: 20723242] (Neutralization, Human)
• Rodier F, Coppe JP, Patil CK, Hoeijmakers WA, Munoz DP, Raza SR, Freund A, Campeau E, Davalos AR, Campisi J Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion. Nat. Cell Biol., 2009-07-13;11(8):973-9. 2009-07-13 [PMID: 19597488] (ICC, Human)
• Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. J. Allergy Clin. Immunol., 2008-12-05;123(1):239-48. 2008-12-05 [PMID: 19058839] (Flow Cytometry, Human)
• Chong DY, Boehlke CS, Zheng QD, Zhang L, Han Y, Zacks DN Interleukin-6 as a photoreceptor neuroprotectant in an experimental model of retinal detachment. Invest. Ophthalmol. Vis. Sci., 2008-03-31;49(7):3193-200. 2008-03-31 [PMID: 18378582] (Neutralization, Mouse, Rat)
• Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis. Histopathology, 2005-06-01;46(6):659-67. 2005-06-01 [PMID: 15910597] (ICC, Human)

FAQs for IL-6 Antibody (MAB2061). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6
• Uniprot:
  • P05231()