IL-6 Antibody (IL6/1270)

Immunohistochemistry-Paraffin: IL-6 Antibody (IL6/1270) [NBP2-44953] - Human Colon Carcinoma stained with IL-6 Monoclonal Antibody (IL6/1270).

Immunohistochemistry-Paraffin: IL-6 Antibody (IL6/1270) [NBP2-44953] - Human Esophageal Carcinoma stained with IL-6 Monoclonal Antibody (IL6/1270).

Product Discontinued

IL-6 Antibody (IL6/1270) Summary

• Immunogen: Recombinant IL-6 protein
• Localization: Cytoplasmic and extracellular (secreted)
• Specificity: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. It plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. It is involved in host defense, acute phase reactions, immune responses, and hematopoiesis.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: IL6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30799288).
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL-6 Antibody (IL6/1270)

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6 Antibody (NBP2-44953)(6)

Publications using NBP2-44953

• Thompson PJ, Shah A, Ntranos V et al. Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes Cell Metab. 2019-02-14 [PMID: 30799288] (ICC/IF, Mouse)
• Tang R, Paul C, Lattanzio R, et al. Partial loss of colonic primary cilia promotes inflammation and carcinogenesis bioRxiv 2019-12-29 (IHC-P, Human, Mouse)
• Jiang Z, Li H, Schroer SA et al. Hypophosphorylated pRb knock-in mice exhibit hallmarks of aging and vitamin C-preventable diabetes The EMBO journal 2022-01-13 [PMID: 35023164] (IHC-P, Mouse)
• Ding Z, Qiu M, Alharbi M et al FOXO1 expression in chondrocytes modulates cartilage production and removal in fracture healing Bone 2021-03-04 [PMID: 33662610] (FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Bain, W G, PeNaloza, H F Et al. Lower Respiratory Tract Myeloid Cells Harbor SARS-Cov-2 and Display an Inflammatory Phenotype. Chest 2021-03-01 [PMID: 33217417] (ICC/IF, Human)
• Thompson PJ, Shah A, Apostolopolou CC, Bhushan A BET proteins are required for transcriptional activation of the senescent beta cell secretome in Type 1 Diabetes Int J Mol Sci 2019-09-29 [PMID: 31561444]

FAQs for IL-6 Antibody (NBP2-44953). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6
• Uniprot:
  • P05231()