Recombinant Human IL12A/IL12B His Protein

SDS-Page: Recombinant Human IL12A/IL12B Protein [NBP2-51854] - 3ug by SDS-PAGE visualized by coomassie blue stain.Lane 1 : reducing conditionLane 2 : non-reducing condition

Recombinant Human IL12A/IL12B Protein [NBP2-51854] - Human IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range < 1 ng/ml.

Product Discontinued

Recombinant Human IL12A/IL12B His Protein Summary

• Description: A bioactive recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 23-328/23-219 of Human IL12A/IL12B
  
  

  Source:Baculovirus

  Amino Acid Sequence: * IL-12 is a heterodimer of IL12A and IL12B linked through a disulfide-bond between cysteines in below sequences.
  IL12A(p35)
  RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH
  IL12B(p40)
  IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

• Details of Functionality: Human IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range is less than or equal to 1 ng/ml.
• Source: Baculovirus
• Protein/Peptide Type: Biologically Active Protein
• Gene: IL12A
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 34.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human IL12A/IL12B His Protein

• CLMF p35
• CLMF p40
• CLMF
• CLMF2
• cytotoxic lymphocyte maturation factor 1, p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12, subunit p35
• IL-12A
• IL-12B
• IL35 subunit
• IMD28
• IMD29
• interleukin 12, p35
• interleukin 12, p40
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• interleukin-12 alpha chain
• interleukin-12 beta chain
• natural killer cell stimulatory factor, 40 kD subunit
• NF cell stimulatory factor chain 1
• NFSK
• NK cell stimulatory factor chain 1
• NK cell stimulatory factor chain 2
• NKSF
• NKSF1
• NKSF2
• p35

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Bioinformatics

• Gene Symbol: IL12A