Importin alpha 2/KPNA2 Antibody - BSA Free

Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Detection of human RCH1Importin alpha 2/KPNA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCH1 antibody NB100-79807 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCH1 was also immunoprecipitated by a previous lot of NB100-79807 (lot 1) and by rabbit anti-RCH1 antibody NB100-79808, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Importin alpha 2/KPNA2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAG cohort 1 (Karyopherin Alpha 2) using the numbering given in entry NP_002257.1 (GeneID 3838).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KPNA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 -1:2000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:5000-1:15000
• Application Notes: Use in Immunoprecipitation reported in scientific literature (PMID 25977366). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  Importin alpha 2/KPNA2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Importin alpha 2/KPNA2 Antibody - BSA Free

• Importin alpha 1
• Importin alpha 2
• importin-alpha-P1
• IPOA1
• karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• Karyopherin subunit alpha-2
• KPNA2
• Pendulin
• QIP2
• QIP2importin alpha 2
• RAG cohort 1
• RAG cohort protein 1
• RCH1
• RCH1importin subunit alpha-2
• SRP1
• SRP1alpha
• SRP1-alpha

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin alpha 2/KPNA2 Antibody (NB100-79807)(7)

Publications using NB100-79807

• Chu TLH, Connell M, Zhou L et al. Cell Cycle-dependent Tumor Engraftment and Migration are Enabled by Aurora A. Mol. Cancer Res. 2017-10-09 [PMID: 28993511] (Human)
• Jeong SA, Kim K, Lee JH et al. Akt-mediated phosphorylation increases the binding affinity of hTERT for importin alpha to promote nuclear translocation. J. Cell. Sci. 2015-05-21 [PMID: 25999477] (WB, Human)
• Yajima M, Wessel GM. Essential elements for translation: the germline factor Vasa functions broadly in somatic cells. Development. 2015-06-01 [PMID: 25977366] (WB, IP)
• Ishiguro K, Ando T, Maeda O et al. Cutting edge: tubulin alpha functions as an adaptor in NFAT-importin beta interaction. J Immunol 2011-03-01 [PMID: 21278340]
• Jeffrey RA, Ch'ng TH, O'Dell TJ et al. Activity-dependent anchoring of importin alpha at the synapse involves regulated binding to the cytoplasmic tail of the NR1-1a subunit of the NMDA receptor. J Neurosci 2009-12-01 [PMID: 20016075]
• Sato H, Maquat LE. Remodeling of the pioneer translation initiation complex involves translation and the karyopherin importin beta. Genes Dev 2009-11-01 [PMID: 19884259]
• Chu TLH Aurora kinase A, regulated by HMMR and TPX2, controls cell migration and engraftment via centrosome polarization Thesis 2020-01-01 (WB, Human)

Bioinformatics

• Gene Symbol: KPNA2
• Entrez:
  • 3838(Human)
• Uniprot:
  • NP_002257.1(Human)