Insulin Antibody

Western Blot: Insulin Antibody [NBP1-45662] - Analysis using recombinant human Insulin.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Insulin Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab286162)
• Immunogen: E. coli expressed recombinant human Insulin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INS
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 4 ug/mL

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.5% BSA, 30% glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for Insulin Antibody

• IDDM2
• ILPR
• INS
• Insulin
• IRDN
• MODY10
• proinsulin

Background

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Insulin Antibody (NBP1-45662)(1)

Publication using NBP1-45662

• Rodriguez-Rodriguez P, Sandebring-Matton A, et al. Tau hyperphosphorylation induces oligomeric insulin accumulation and insulin resistance in neurons. Brain 2017-12-01 [PMID: 29053786] (WB, Rat)

FAQs for Insulin Antibody (NBP1-45662). (Showing 1 - 2 of 2 FAQ).

1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
   • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.
2. Were special non-denaturing and/or non-reducing conditions used to obtain the WB image for your insulin Ab? The band you show would suggest that the insulin di-peptide is intact (disulfides).
   • Our WB image for NBP1-45662 was generated from testing performed on a partial recombinant insulin protein. The protein is 50 amino acids in length, which corresponds to the ~5.5kDa observed weight.

Bioinformatics

• Gene Symbol: INS
• Entrez:
  • 3630(Human)
• Uniprot:
  • P01308()