Integrin alpha V/CD51 Antibody (P2W7) - BSA Free

Immunocytochemistry/Immunofluorescence: Integrin alpha V/CD51 Antibody (P2W7) [NB100-2618] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Integrin alpha V/CD51 Antibody [P2W7] (NB100-2618) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: P2W7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Integrin alpha V/CD51 Antibody (P2W7) - BSA Free Summary

• Immunogen: An ocular melanoma cell line (V+B2) expressing high levels of human integrin alpha V beta 2.
• Localization: Type I membrane protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAV
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence 8-25 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa. This antibody is CyTOF ready.
• Theoretical MW: 155 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha V/CD51 Antibody (P2W7) - BSA Free

• antigen identified by monoclonal L230
• CD51 antigen
• CD51
• Integrin alpha V
• integrin alpha-V
• integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• ITGAV
• MSK8
• Vitronectin Receptor alpha
• Vitronectin receptor subunit alpha
• VNRA
• VNRADKFZp686A08142

Background

Integrin alpha V chain interacts with the integrin beta 3 subunit/CD61 to form the alpha-V-beta-3 heterodimer/vitronectin receptor. It is expressed on endothelial cells, some activated leukocytes, NK cells, macrophages, neutrophils, and platelets. Integrin alpha V also forms heterodimers with the integrin beta 1, beta 5, beta 6, and beta 8 subunits. Alpha-V-beta-3 is an activation dependent receptor for platelet attachment and spreading on vitronectin and other matrix components.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha V/CD51 Antibody (NB100-2618)(1)

Publication using NB100-2618

• Jin S, Lee WC, Aust D et al. beta 8 integrin mediates pancreatic cancer cell radiochemoresistance Mol. Cancer Res. 2019-07-23 [PMID: 31337672] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ITGAV
• Entrez:
  • 3685(Human)