Integrin beta 3/CD61 Antibody (SJ19-09)

Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysateLane 2: mouse marrow tissue lysate

Immunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse lung tissue. IF-stained paraffin section. Image from verified customer review.

Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse bone marrow cell lysate with DMSO (lane 1), chemical agonist #1 (lane 2), chemical agonist #2 (lane 3), chemical agonist #3 (lane 4). WB image submitted by a verfied customer review.

Immunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human spleen tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse testis tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: SJ19-09
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Integrin beta 3/CD61 Antibody (SJ19-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Integrin beta 3/CD61 aa 729-788 / 788. (SwissProt: P05106 Human; SwissProt: O54890 Mouse; Entrez Gene: 29302 Rat)
• Localization: Cell membrane, Cell projection, Cell junction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin beta 3/CD61 Antibody (SJ19-09)

• CD61 antigen
• CD61
• GP3A
• GP3Aplatelet glycoprotein IIIa
• GPIIIA
• GPIIIaintegrin beta-3
• INGRB3
• Integrin beta 3
• integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• ITG B3
• ITGB3
• Platelet membrane glycoprotein IIIa

Background

Platelet membrane glycoprotein Integrin beta-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. Homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors. (1) Data provide further support for the key role of the cytoplasmic domain of the beta3 integrin in cell adhesion and suggest a potential role for the beta3C integrin subunit in modulating cell-matrix interactions (2). It has been demonstrated that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3). be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 3/CD61 Antibody (NBP2-67416)(7)

Publications using NBP2-67416

• Wang Y Plasmatic von Willebrand factor regulates hematogenous metastasis of melanoma cells through its binding to heparan sulfate Thesis 2023-01-01 (Immunocytochemistry/ Immunofluorescence)
• Chee E, Mihalko E, Nellenbach K et al. Wound-triggered shape change microgels for the development of enhanced biomimetic function platelet-like particles Journal of biomedical materials research. Part A 2023-10-17 [PMID: 37846887] (ICC/IF, Mouse)
• Mei S, Tang R, Hu Y et Al. Integrin ?3 Mediates Sepsis and Mechanical Ventilation-Associated Pulmonary Fibrosis Through Glycometabolic Reprogramming Lab Invest 2023-02-08 [PMID: 36748196]
• Mei S, Xu Q, Hu Y et al. Integrin B3-PKM2 pathway-mediated aerobic glycolysis contributes to mechanical ventilation-induced pulmonary fibrosis Theranostics 2022-08-15 [PMID: 36168620] (IF/IHC, ICC/IF, WB, Mouse)
• Mihalko E, Nellenbach K, Krishnakumar M et al. Fibrin specific poly(N isopropylacrylamide) nanogels for targeted delivery of tissue type plasminogen activator to treat thrombotic complications are well tolerated in vivo Bioeng Transl Med 2022-05-23 [PMID: 35600656]
• Zheleznyak A, Mixdorf M, Marsala L Et al. Orthogonal targeting of osteoclasts and myeloma cells for radionuclide stimulated dynamic therapy induces multidimensional cell death pathways Theranostics 2021-06-22 [PMID: 34335961] (WB, Human)
• Mihalko E, Sandry M, Mininni N, et al. Fibrin-modulating nanogels for treatment of disseminated intravascular coagulation Blood Advances 2021-02-09 [PMID: 33560377] (Rat)

Reviews for Integrin beta 3/CD61 Antibody (NBP2-67416) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 09/14/2019

Summary

• Application: Western Blot
• Sample Tested: macrophage cell lysate,mouse primary cell
• Species: Mouse
• Lot: HL1203

reviewed by: Verified Customer immunofluorescence staining Mouse 07/05/2019

Summary

• Application: Other
• Sample Tested: Lung tissue
• Species: Mouse
• Lot: HL1203

Comments

• Comments: Paraffin Section

Bioinformatics

• Gene Symbol: ITGB3