Irisin/FNDC5 Antibody - BSA Free

Western Blot: Irisin/FNDC5 Antibody [NBP2-59680] - Recombinant human Irisin protein was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Irisin/FNDC5 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-59680] - IHC analysis of a formalin fixed and paraffin embedded human kidney tissue section (NBP1-78269) using 1:200 dilution of Irisin/FNDC5 antibody. The signal was developed with HRP-DAB detection and the sections were counterstained with hematoxylin. The antibody generated a membrane cytoplasmic staining in almost all of the cell types in the tested section.

• Reactivity: Hu, Mu, Ma
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Irisin/FNDC5 Antibody - BSA Free Summary

• Immunogen: Partial recombinant protein made to an internal region of the human Irisin/FNDC5 protein (amino acids 32-143) [UniProt Q8NAU1]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FNDC5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 0.5 - 1.0 ug/ml
• Application Notes: Irisin/FNDC5 has a theoretical molecular weight of 23 kDa, however this protein is highly glycosylated and will run higher in WB.

Reactivity Notes

Use in Camel reported in scientific literature (PMID:33424357)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Irisin/FNDC5 Antibody - BSA Free

• fibronectin type III domain containing 5
• fibronectin type III domain-containing protein 5
• FNDC5
• FRCP2
• FRCP2Fibronectin type III repeat-containing protein 2
• Irisin

Background

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development. Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation. Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise. Irisin induces expression of peroxisome proliferator-activated receptor gamma, co-activator PGC1 alpha and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes. A similar function in humans is suggested. Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Irisin/FNDC5 Antibody (NBP2-59680)(4)

Publications using NBP2-59680

• Maha Saber, Amanda D. Rice, Immaculate Christie, Rebecca G. Roberts, Kenneth S. Knox, Peter Nakaji, Rachel K. Rowe, Ting Wang, Jonathan Lifshitz Remote ischemic conditioning reduced acute lung injury after traumatic brain injury in the mouse Shock (Augusta, Ga.) 2021-02-01 [PMID: 32769821] (Immunohistochemistry, Human)
• Bi J, Zhang J, Ren Y et al. Irisin reverses intestinal epithelial barrier dysfunction during intestinal injury via binding to the integrin alpha V beta 5 receptor J. Cell. Mol. Med. 2019-11-07 [PMID: 31701659] (IF/IHC, Human)
• Kirbas A, Karakus E, Ozkanlar S et al. FNDC5 Gene Expression in the Smooth Muscles of Dromedary Camels (Camelusdromedarius) Int J Vet Sci 2020-01-01
• Kirat D, Hamada M, Moustafa A, Miyasho T Irisin/FNDC5: A participant in camel metabolism Saudi Journal of Biological Sciences 2021-01-01 [PMID: 33424357] (IHC-P, Camel)

Bioinformatics

• Gene Symbol: FNDC5