Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Isocitrate Dehydrogenase 1/IDH1 Mouse Monoclonal Antibody (Isocitrate Dehydrogenase 1/IDH1/1152).

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302] - Human Colon Carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted [NBP2-48302] - Human Prostate Carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).

• Reactivity: Hu
• Applications: IHC
• Clone: IDH1/1152
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant fragment of human Isocitrate Dehydrogenase 1/IDH1 protein (around aa 281-414) (exact sequence is proprietary) (Uniprot: O75874 )
• Localization: Cytoplasmic & Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDH1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) - IHC-Prediluted

• Cytosolic NADP-isocitrate dehydrogenase
• EC 1.1.1.42
• IDCD
• IDH
• IDH1
• IDP
• IDPC
• isocitrate dehydrogenase [NADP] cytoplasmic
• isocitrate dehydrogenase 1 (NADP+), soluble
• Isocitrate Dehydrogenase 1
• NADP(+)-specific ICDH
• NADP-dependent isocitrate dehydrogenase, cytosolic
• NADP-dependent isocitrate dehydrogenase, peroxisomal
• Oxalosuccinate decarboxylase
• PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDH1