IDH2 Antibody - BSA Free

Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.

Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.

Western Blot: IDH2 Antibody [NBP2-22166] - Analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a concentration of 2 ug/mL.

Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

• Reactivity: Hu, Mu, Rt, Gp, Ha, Pm, Rb
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IDH2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human IDH2 protein (between residues 375-452) [UniProt P48735].
• Localization: Mitochondria
• Predicted Species: Guinea Pig (100%), Rabbit (100%), Hamster (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IDH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Western Blot 1 - 2 ug/mL
• Application Notes: In Western blot a band is observed ~48 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

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• Control Peptide:

  IDH2 Peptide (NBP2-22166PEP)

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Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IDH2 Antibody - BSA Free

• D2HGA2
• EC 1.1.1.42
• ICD-M
• IDH
• IDHM
• IDP
• IDPM
• isocitrate dehydrogenase [NADP], mitochondrial
• isocitrate dehydrogenase 2 (NADP+), mitochondrial
• mNADP-IDH
• NADP(+)-specific ICDH
• Oxalosuccinate decarboxylase

Background

Isocitrate dehydrogenase 2, also known as IDH, IDP, oxalosuccinate decarboxylase, IDPM, and mNADP-IDH, catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is imperative for metabolic regulation and energy production, primarily via the citric acid cycle. In addition, IDH2 has been implicated in tight interactions with the pyruvate dehydrogenase complex. IDH2 is localized in the mitochondria and is found in stems, roots and leaves. Recent research has found that mutations in IDH2 can block histone demethylation and result in an inability for cells to differentiate in gliomas (PMID: 22343901). Further studies have also linked IDH2 to both gallbladder and bile duct cancer (PMID: 22180306).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IDH2 Antibody (NBP2-22166)(5)

Publications using NBP2-22166

• Lin S, Yin S, Shi J et al. Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration Bioactive Materials 2022-12-01 [PMID: 35387176] (IF/IHC, Mouse)
• Oyama Y, Bartman CM, Bonney S et al. Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium Cell Rep 2019-08-08 [PMID: 31390562] (WB, Human)
• Kim YR, Kim KH, Lee S et al. Expression patterns of members of the isocitrate dehydrogenase gene family in murine inner ear Biotech Histochem 2017-09-19 [PMID: 28925723] (Mouse)
• Saha SK, Gordan JD, Kleinstiver BP et al. Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC-dependence in intrahepatic cholangiocarcinoma. Cancer Discov. 2016-05-26 [PMID: 27231123] (WB, Human)
• Saha SK, Parachoniak CA, Ghanta KS et al. Mutant IDH inhibits HNF-4alpha to block hepatocyte differentiation and promote biliary cancer. Nature 2014-09-04 [PMID: 25043045] (WB)

Reviews for IDH2 Antibody (NBP2-22166) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 01/02/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: 293T
• Species: Human
• Lot: A-1

reviewed by: Verified Customer WB Human 01/02/2020

Summary

• Application: Western Blot
• Sample Tested: 293T
• Species: Human
• Lot: A-1

Bioinformatics

• Gene Symbol: IDH2
• Entrez:
  • 3418(Human)
  • 269951(Mouse)
  • 361596(Rat)
• Uniprot:
  • P48735(Human)
  • P54071(Mouse)
  • P56574(Rat)