Jak1 Antibody (7A5) - BSA Free

Immunohistochemistry: Jak1 Antibody (7A5) [NBP3-26188] - Image of Jak1 Antibody (7A5) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: 7A5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Jak1 Antibody (7A5) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Jak1 [UniProt P23458]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: JAK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Jak1 Antibody (7A5) - BSA Free

• EC 2.7.10
• Jak1
• JAK-1
• JAK1Atyrosine-protein kinase JAK1
• JAK1B
• Janus kinase 1EC 2.7.10.2
• JTK3

Background

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterised by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signalling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JAK1