Ki67/MKI67 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB500-170] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-170 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Simple Western: Ki67/MKI67 Antibody [NB500-170] - Detection of Ki67/MKI67 by Simple WesternTM. Simple Western lane view shows lysates of HeLa parental cell line and Ki67 knockout (KO) HeLa cell line. A specific band was detected for Ki67/MKI67 at approximately 320 kDa (as indicated) in the parental cell line, but is not detectable in the knockout HeLa cell line using 20 ug/mL of Rabbit Anti-Ki67/MKI67 Polyclonal Antibody (Catalog # NB500-170). GAPDH is shown as a loading control. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry: Ki67/MKI67 Antibody [NB500-170] - Senescent MSPCs are characterized by loss of nestin expression. Double-immunofluorescence images of femoral metaphysis sections from 4-, 6-, 8-, and 12-week-old male Nestin-GFP mice using antibodies against GFP (green) and Ki67 (red). DAPI stains nuclei blue. GP growth plate. PS primary spongiosa. Quantification of the percentage of GFP+ cells that express Ki67. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01509-0) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB500-170] - Ki-67/MKI67 Antibody [NB500-170] - Tissue section of human tonsil using 1:50 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Ki67 antibody generated a specific nuclear staining in the cells in germinal centers of the tested tonsil tissue.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB500-170] - Ki-67/MKI67 Antibody [NB500-170] - Human tonsil using 1:200 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Ki67 antibody generated a specific nuclear staining in the cells in germinal centers of the tested tonsil tissue.

Western Blot: Ki67/MKI67 Antibody - BSA Free [NB500-170] - Whole cell lysates from SUM159, MDA-MB-231 and MCF-7 cells were loaded with 30 ug/lane. 10% SDS-PAGE. Ki67/MKI67 antibody (NB500-170) was used for primary antibody: 1:2000, 4C, overnight. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB500-170] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Ki67/MKI67 Antibody NB500-170 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: Ki67/MKI67 Antibody - BSA Free [NB500-170] - An intracellular stain was performed on U-251 MG cells with Ki67/MKI67 Antibody NB500-170AF594 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Western Blot: Ki67/MKI67 Antibody [NB500-170] - Ki-67/MKI67 Antibody [NB500-170] - Analysis of A431 (A) and Hek293 (B) cell lysate using Ki67 antibody (NB500-170) at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB500-170] - Ki67 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB500-170] - Confocal immunofluorescent analysis of MCF7 cells using Ki67 antibody (NB500-170, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB500-170] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-170 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB500-170] - Ki-67/MKI67 Antibody [NB500-170] - Human tonsil.

Ki67/MKI67 was detected in immersion fixed MCF7 human breast cancer cell line using Rabbit anti-Ki67/MKI67 Affinity Purified Polyclonal Antibody conjugated to Biotin (Catalog # NB500-170B) at 5 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Ki67/MKI67 was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti- Ki67/MKI67 Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NB500-170AF488) (green) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

An intracellular stain was performed on A431 human skin carcinoma cell line using Rabbit anti-Ki67/MKI67 Affinity Purified Polyclonal Antibody conjugated to DyLight 650 (Catalog # NB500-170C, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.

Ki67/MKI67 was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti- Ki67/MKI67 Affinity Purified Polyclonal Antibody conjugated to DyLight 488 (Catalog # NB500-170G) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Ki67/MKI67 was detected in immersion fixed U-251 MG human glioblastoma cell line using Rabbit anti- Ki67/MKI67 Affinity Purified Polyclonal Antibody conjugated to DyLight 650 (Catalog # NB500-170C) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB500-170] - PKD2 enzymatic deficiency had no effect on the proliferation or apoptosis of intestinal epithelial cells in mice exposed to DSS.(a) Representative 200× immunohistochemical images (left) & quantification of Ki-67+ proliferating intestinal epithelial cells (right) in wild-type & PKD2SSAA/SSAA mice. Bar represents 5 μm. (b) Representative 200× immunohistochemical images (left) & quantification of cleaved Caspase-3+ apoptotic intestinal epithelial cells (right) in wild-type & PKD2SSAA/SSAA mice. Bar represents 5 μm. Results are representative of three separate experiments with three mice per group. All p > 0.05 as measured by one way-ANOVA among four groups. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep34079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Ki67/MKI67 Antibody - BSA Free [NB500-170] - Deletion of Vangl2, but not Vangl1, in the mammary gland results in narrow ducts & low cell turnover. (A) RT-qPCR analysis of Vangl1 & Vangl2 mRNA levels in the mammary glands of MMTV-Cre, MMTV-Cre;Vangl2flox/+ & MMTV-Cre;Vangl2flox/flox at 10 weeks of age. (B) Representative images of 10 week old, carmine stained mammary glands & quantification of total branch number from MMTV-Cre, MMTV-Cre;Vangl2flox/+ & MMTV-Cre;Vangl2flox/flox mice (n = 3 mice per genotype). (C) Histological analysis by H&E staining show normal duct formation in 10 week old MMTV-Cre & MMTV-Cre;Vangl2flox/+ glands, whereas ducts from MMTV-Cre;Vangl2flox/flox glands are narrow & have significantly diminished lumens (n = 3 mice/genotype). (D) Immunostaining in mammary ducts show normal distribution of luminal (Cytokeratin 8 (K8), green), & basal (K5), red; SMA magenta) cell populations. (E) Immunofluorescence of adhesion junctional protein E-Cadherin (CDH1, green) & apical membrane marker pERM (red). (F,G) Immunostaining in 10 week old MMTV-Cre;Vangl2+/+ & MMTV-Cre;Vangl2flox/flox glands & quantitation of (F) Ki67 & (G) Cleaved Caspase 3 (n = 3–5 mice per genotype). Lu indicates lumen. * indicates non-magnified duct. Data are represented as mean +/− SEM. Scale bar represents 100 μm (C,F,G) 50 μm (D) & 10 μm (E). Student’s t-test *p < 0.05 & ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31068622), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB500-170] - Senescent MSPCs are characterized by loss of nestin expression. a, b Representative images of GFP immunofluorescence staining (green) & quantitative analysis of GFP+ cells in femoral primary spongiosa from 4, 6, 8, & 12-week-old male Nestin-GFP mice. Images in upper panels in a are lower power w/ boxes outlining area of higher power in lower panels. Numbers of GFP+ cells per mm2 tissue area in primary spongiosa (N. GFP+ cells/PS.Ar) b. 4W, 6W, 8W, & 12W represent 4-, 6-, 8-, & 12-week-old mice, respectively. DAPI stains nuclei blue. c, d Representative images of flow cytometry analysis (c) & % of CD45−GFP+ cells (d) in femoral metaphysis from 4-, 6-, 8-, & 12-week-old male Nestin-GFP mice. e, f Double-immunofluorescence images of femoral metaphysis sections from 4-, 6-, 8-, & 12-week-old male Nestin-GFP mice using antibodies against GFP (green) & Ki67 (red) e. DAPI stains nuclei blue. GP growth plate. PS primary spongiosa. Quantification of % of GFP+ cells that express Ki67 f. Five mice per group. Data are represented as mean ± s.e.m. *P < 0.05 as determined by ANOVA. g Diagram showing isolation of Nestin-GFP+ (red) & Nestin-GFP− (purple) mesenchymal stem/progenitor cells (MSPCs) by fluorescence-activated cell sorting. Detailed information on isolation of MSPCs from femoral metaphysis from Nestin-GFP mice are described in Supplementary Fig. 3 & Methods section. h–j The sorted cells cultured, & SA-beta Gal staining (h), BrdU incorporation (i), & p16INK4a immunostaining (j) performed, & representative images shown. k–m Quantification of % of cells that express SA-beta Gal (k), BrdU (l), & p16INK4a (m). n = 5. Data are represented as mean ± s.e.m. *P < 0.01 as determined by Student’s t-tests Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/29101351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB500-170] - Cellular senescence occurs in primary spongiosa of long bone during late puberty. a–e Representative senescence-associated beta -galactosidase (SA-beta Gal) staining (blue) & quantitative analysis of SA-beta Gal+ cells in femoral metaphysis (a–c) & diaphysis (d, e) sections from increasing ages of male mice. 4, 6, 8, & 12W represent 4-, 6-, 8-, & 12-week-old mice, respectively. Images in a are lower power with boxes outlining the area of higher power in b. Numbers of SA-beta Gal+ cells per mm2 tissue area in primary spongiosa (N. SA-beta Gal+ cells/PS.Ar) (c) & diaphysis (N. SA-beta Gal+ cells/DP.Ar) (e). Counterstained with eosin (pink). f, g Representative images of immunofluorescence staining (f) & quantitative analysis of ki67+ (g) cells (red) in femoral primary spongiosa from 4, 6, 8, & 12-week-old male mice. DAPI stains nuclei blue. Images in upper panels in f are lower power with boxes outlining the area of higher power in lower panels. Five mice per group. Data are represented as mean ± s.e.m. Ar tissue area, DP diaphysis, GP growth plate, N number, PS primary spongiosa. *P < 0.01 as determined by ANOVA Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29101351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Av
• Applications: WB, Flow, ICC/IF, IHC, IP, WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Ki67/MKI67 Antibody - BSA Free Summary

• Immunogen: The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Human KI67/MKI67, between amino acids: 1550-1600 [Uniprot: P46013].
• Localization: Nuclear
• Marker: Proliferation Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MKI67
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 34946963)
  • Immunoblotting reported in scientific literature (PMID 24248265)
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:50-1:300
  • Immunohistochemistry-Frozen 1:50-1:300. Use reported in scientific literature (PMID 22493503)
  • Immunohistochemistry-Paraffin 1:50-1:300
  • Immunoprecipitation reported by customer review
  • Knockout Validated
  • Western Blot 1-2 ug/ml
• Application Notes: Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer (pH 6.0) prior to immunostaining. This antibody will not work without optimal antigen retrieval, and is a critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein.
• Theoretical MW: 359 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Avian reported in scientific literature (PMID:34986451) Ki67/MKI67 Antibody reacted with Mouse (PMID: 22033079), Rat (PMID: 22521325), and Porcine (PMID: 29146772). .

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ki67/MKI67 Antibody - BSA Free

• antigen identified by monoclonal Ki-67
• Antigen Ki67
• antigen KI-67
• antiKi67
• Ki67 ihc
• Ki-67 ihc
• Ki67 mouse
• Ki-67 mouse
• Ki67 western blot
• Ki-67 western blot
• Ki67
• Ki-67
• KIA
• Marker Of Proliferation Ki-67
• MIB-
• MIB-1
• MKI67
• PPP1R105
• Proliferation Marker Protein Ki-67
• proliferation-related Ki-67 antigen
• Protein Phosphatase 1
• Regulatory Subunit 105
• TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ki67/MKI67 Antibody (NB500-170)(191)

Publications using NB500-170

• Zou Y, Guo S, Wen L et al. Targeting NAT10 inhibits osteosarcoma progression via ATF4/ASNS-mediated asparagine biosynthesis Cell Reports Medicine 2024-09-17 [PMID: 39293390]
• Zhang Y, Liu G, Zeng Q, Wu W et Al. CCL19-producing fibroblasts promote tertiary lymphoid structure formation enhancing anti-tumor IgG response in colorectal cancer liver metastasis Cancer Cell 2024-08-13 [PMID: 39137726]
• Chang TT, Lin LY, Chen C et Al. CCL4 contributes to aging related angiogenic insufficiency through activating oxidative stress and endothelial inflammation Angiogenesis 2024-05-13 [PMID: 38739303]
• Xiong G, Chen Z, Liu Q et Al. CD276 regulates the immune escape of esophageal squamous cell carcinoma through CXCL1-CXCR2 induced NETs J Immunother Cancer 2024-01-01 [PMID: 38724465]
• Dejin Zheng, Ahmed A Elnegiry, Chenxiang Luo, Mohammed Amine Bendahou, Liangqi Xie, Diana Bell, Yoko Takahashi, Ehab Hanna, George I Mias, Mayra F Tsoi, Bin Gu Brd4::Nutm1 fusion gene initiates NUT carcinoma in vivo Life Science Alliance 2024-05-09 [PMID: 38724194]
• Tomoki Kawahata, Kitaru Tanaka, Kyohei Oyama, Jun Ueda, Kensaku Okamoto, Yuichi Makino, Jörn Karhausen HIF3A gene disruption causes abnormal alveoli structure and early neonatal death PLOS ONE 2024-05-08 [PMID: 38717999]
• Zhu M, Fang Y, Cheng Y et Al. The Alleviating Effect of Taxifolin on Deoxynivalenol-Induced Damage in Porcine Intestinal Epithelial Cells Vet Sci 2024-03-30 [PMID: 38668423]
• Cheng M, Chen S, Li K et Al. CD276-dependent efferocytosis by tumor-associated macrophages promotes immune evasion in bladder cancer Nat Commun 2024-04-01 [PMID: 38561369]
• Xiong G, Xie N, Nie M et Al. Single-cell transcriptomics reveals cell atlas and identifies cycling tumor cells responsible for recurrence in ameloblastoma Int J Oral Sci 2024-02-29 [PMID: 38424060]
• Dsilva A, Avlas S, Rhone N et Al. A Mouse Model for Eosinophilic Esophagitis (EoE) Curr Protoc 2024-02-20 [PMID: 38372429]
• Xi G, Feng P, Zhang X et Al. iPSC-derived cells stimulate ABCG2(+)/NES(+) endogenous trabecular meshwork cell proliferation and tissue regeneration Cell Prolif 2024-02-14 [PMID: 38356373]
• Hakan Kula, Orkun Ilgen, Sefa Kurt, Filiz Yılmaz Patterns of proliferation and fibrosis in a rat model of endometriosis following administration of Allium cepa Clinical and Experimental Reproductive Medicine 2024-06-01 [PMID: 38263589]
• Wang X, Ling R, Peng Y et Al. RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac(4)C modification Int J Oral Sci 2024-01-22 [PMID: 38246918]
• Chen S, Yu Y, Xie S et al. Local H2 release remodels senescence microenvironment for improved repair of injured bone Nature communications 2023-11-27 [PMID: 38012166] (ICC/IF)
• Juan Zhou, Yiran Xu, Luyao Wang, Yu Cong, Ke Huang, Xinxing Pan, Guangquan Liu, Wenqu Li, Chenchen Dai, Pengfei Xu, Xuemei Jia LncRNA IDH1-AS1 sponges miR-518c-5p to suppress proliferation of epithelial ovarian cancer cell by targeting RMB47 Journal of Biomedical Research 2024-01-01 [PMID: 37981573]
• Xiang L, Wei H, Ye W et al. Prolyl hydroxylase 2 inhibits glycolytic activity in colorectal cancer via the NF??B signaling pathway International journal of oncology 2024-01-01 [PMID: 37975227] (IHC-P, Human)
• Alsaidan OA, Onobun E, Ye C et al. Inhibition of N-myristoyltransferase activity promotes androgen receptor degradation in prostate cancer The Prostate 2023-10-31 [PMID: 37905842] (IHC, Human)
• Newell AJ, Patisaul HB Developmental organophosphate flame retardant exposure disrupts adult hippocampal neurogenesis in Wistar rats Neurotoxicology 2023-09-30 [PMID: 37783313] (IHC, Mouse)
• Yan L, Fang YX, Lu EQ et al. Extracellular Glutamine Promotes Intestinal Porcine Epithelial Cell Proliferation via Arf1-mTORC1 Pathway Independently of Rag GTPases Journal of agricultural and food chemistry 2023-10-04 [PMID: 37738360]
• Galhenage P, Zhou Y, Perry E, Loc B et Al. Replication stress and defective checkpoints make fallopian tube epithelial cells putative drivers of high-grade serous ovarian cancer Cell Rep 2023-09-20 [PMID: 37729060]
• Atmane MI, Vigneau AL, Beaudry F et al. Therapeutic trial of fluvastatin in a cell line xenograft model of canine mammary gland cancer Veterinary and comparative oncology 2023-09-14 [PMID: 37709554] (IHC, Mouse)
• Tian S, Paudel D, Hao F et al. Refined fiber inulin promotes inflammation-associated colon tumorigenesis by modulating microbial succinate production Cancer reports (Hoboken, N.J.) 2023-07-25 [PMID: 37489647]
• Grenier SF, Khan MW, Reil KA et al. VAX014, an Oncolytic Therapy, Reduces Adenomas and Modifies Colon Microenvironment in Mouse Model of CRC International journal of molecular sciences 2023-06-10 [PMID: 37373142] (IHC-P, Mouse)
• Yu B, Zhou Y, He J TRIM13 inhibits cell proliferation and induces autophagy in lung adenocarcinoma by regulating KEAP1/NRF2 pathway Cell cycle (Georgetown, Tex.) 2023-05-28 [PMID: 37245083] (Immunohistochemistry)
• Abouelkheir M, Taher I, Eladl A et al. Detection and Quantification of Some Ethanol-Producing Bacterial Strains in the Gut of Mouse Model of Non-Alcoholic Fatty Liver Disease: Role of Metformin Pharmaceuticals 2023-04-27 [PMID: 37242441] (IHC-P, Mouse)
• Scalzone A, Cerqueni G, Wang XN et al. A cytokine-induced spheroid-based in vitro model for studying osteoarthritis pathogenesis Frontiers in Bioengineering and Biotechnology 2023-05-09 [PMID: 37229489]
• Ofek P, Yeini E, Arad G et al. Deoxyhypusine hydroxylase: A novel therapeutic target differentially expressed in short-term vs long-term survivors of glioblastoma International journal of cancer 2023-05-04 [PMID: 37141410]
• Patel PS, Algouneh A, Krishnan R et al. Excessive transcription-replication conflicts are a vulnerability of BRCA1-mutant cancers Nucleic acids research 2023-03-17 [PMID: 36928661] (IHC-P, Human)
  
  Details:
  Dilution used in IHC-P 1:00
• Jia Y, Li HY, Wang J et al. Tripartite motif containing 69 elicits ERK2-dependent EYA4 turnover to impart pancreatic tumorigenesis Journal of Cancer 2023-01-01 [PMID: 36741265]
• Wang X, Li K, Cheng M et al. RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac4C modification bioRxiv 2023-02-12 (Immunohistochemistry, Human)
• Jeong Eun Gong, Ji Eun Kim, So Hae Park, Su Jin Lee, Yun Ju Choi, Sun Il Choi, Young Whan Choi, Hee Seob Lee, Jin Tae Hong, Dae Youn Hwang Anti‑tumor effects of an aqueous extract of Ecklonia cava in BALB/cKorl syngeneic mice using colon carcinoma CT26 cells Oncology Reports 2023-06-01 [PMID: 37165874]
• Jatko JT Effects of a 5-week 100ppb Arsenic Exposure in Drinking Water on Mouse Intestinal Epithelium Thesis 2020-01-01 (IF/IHC, Mouse)
• Araki Y, Asano N, Yamamoto N et al. A validation study for the utility of serum microRNA as a diagnostic and prognostic marker in patients with osteosarcoma Oncology Letters 2023-04-13 [PMID: 37153065] (ICC/IF, Human)
  
  Details:
  Dilutions: 1:200
• Iwasa K, Yamamoto S, Yamashina K et al. Dysfunction of a Peripheral Lipid Sensor Gpr120 Causes Pgd2-microglia-provoked Neuroinflammation Research Square Dec 1 2020 (IHC, Mouse)
• Reil K VAX014 Efficacy in the B16F10 Mouse Melanoma Model: A Novel Oncolytic Immunotherapy for Melanoma Thesis 2020-01-01 (IHC-P, Mouse)
• Manuela Estermann, Ricardo Coelho, Francis Jacob, Yen-Lin Huang, Ching-Yeu Liang, Ana Bela Faia-Torres, Dedy Septiadi, Barbara Drasler, Bedia Begum Karakocak, Irini Magdelina Dijkhoff, Alke Petri-Fink, Viola Heinzelmann-Schwarz, Barbara Rothen-Rutishauser A 3D multi-cellular tissue model of the human omentum to study the formation of ovarian cancer metastasis. Biomaterials 2023-02-15 [PMID: 36689832]
• Wang J, Zhao D, Lei Z et al. TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal Cellular & molecular immunology 2023-02-01 [PMID: 36596873] (WB, Human)
  
  Details:
  Dilution used 1:1000
• Vrathasha V, Nikonov S, Bell B et al. Transplanted human induced pluripotent stem cells- derived retinal ganglion cells embed within mouse retinas and are electrophysiologically functional iScience 2022-10-01 [PMID: 36388952] (IHC-WhMt)
• Kim JS, Lee HL, Jeong JH et al. OR2AT4, an Ectopic Olfactory Receptor, Suppresses Oxidative Stress-Induced Senescence in Human Keratinocytes Antioxidants (Basel, Switzerland) 2022-11-03 [PMID: 36358552] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC 1:200
• Alghamdi TA, Krentz NAJ, Smith N et al. Zmiz1 is required for mature B-cell function and mass expansion upon high fat feeding Molecular metabolism 2022-10-25 [PMID: 36307047] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:200
• Chang TT, Chen C, Lin LY, Chen JW CCL4 Deletion Accelerates Wound Healing by Improving Endothelial Cell Functions in Diabetes Mellitus Biomedicines 2022-08-12 [PMID: 36009510] (IHC-P, Mouse)
• Ye M, Liu Z, Gao C et al. Iturin A Rescued STb-R-Induced Pork Skeletal Muscle Growth Restriction through the Hypothalamic-Pituitary-mTORC1 Growth Axis Animals (Basel) 2022-06-17 [PMID: 35739903]
• Zhan F, Zhang Y, Zuo Q et al. YAP knockdown in combination with ferroptosis induction increases the sensitivity of HOS human osteosarcoma cells to Pyropheophorbide-alpha methyl ester-mediated photodynamic therapy Photodiagnosis and photodynamic therapy 2022-06-12 [PMID: 35705143] (WB, Human)
• Verginadis II, Avgousti H, Monslow J et al. A stromal Integrated Stress Response activates perivascular cancer-associated fibroblasts to drive angiogenesis and tumour progression Nature cell biology 2022-06-01 [PMID: 35654839] (IF/IHC, Mouse)
• Arata Nagasaka, Koji Sakiyama, Yasuhiko Bando, Masahito Yamamoto, Shinichi Abe, Osamu Amano, Thimios A. Mitsiadis Spatiotemporal Gene Expression Regions along the Anterior–Posterior Axis in Mouse Embryos before and after Palatal Elevation International Journal of Molecular Sciences 2022-05-05 [PMID: 35563549]
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  Details:
  Citation using the Azide and BSA Free version of this antibody.
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  Figure 4. C-C
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  Details:
  Citation using the Azide and BSA Free form of this antibody.
• Baek H, Kim S, Choi E et al. Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1-associated Mammary Tumor Formation. Int. J. Biol. Sci. 2018-10-25 [PMID: 30416390] (WB, Mouse)
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  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
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  Details:
  This Ki67/MKI67 antibody was used (dilution 1:100) for IHC-P in Spraque Dawley rats subjected to intra-articular injection of double-stranded miR-210 (for control group using control dsRNA) with atelocollagen administered immediately after injury (injured meniscal healing in a rat model) - 4%PFA fixation, 4 um sections, and blocked with horse serum (Figure 4).
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  Details:
  This citation used the FITC version of this antibody.
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Reviews for Ki67/MKI67 Antibody (NB500-170) (10)

Average Rating: 4.3

(Based on 10 reviews)

We have 10 reviews tested in 2 species: Human, Mouse.

reviewed by: Shinford Sun WB Human 08/10/2023

Summary

• Application: Western Blot
• Sample Tested: lung cancer cells
• Species: Human
• Lot: D133925

reviewed by: Nick Dordai WB Human 12/01/2022

Summary

• Application: Western Blot
• Sample Tested: MCF-7,MDA-MB-231,Sum159
• Species: Human
• Lot: D103905-1

reviewed by: Verified Customer IP Human 04/27/2021

Summary

• Application: Immunoprecipitation
• Sample Tested: a number of human cell lines
• Species: Human
• Lot: I

Comments

• Comments: 2 ul antibody used for IP which turned out very good.

reviewed by: Verified Customer WB Human 04/21/2021

Summary

• Application: Western Blot
• Sample Tested: hTERT RPE-1 cell line from ATCC
• Species: Human
• Lot: I

Comments

• Comments: 1:1000 in 5% BSA

reviewed by: Lang Zhao IHC-P Mouse 03/17/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse aortic root
• Species: Mouse
• Lot: CGVS0116042

reviewed by: Alexandra Ambrico Immunocytochemistry/Immunofluorescence Mouse 10/11/2016

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Sample Tested: mouse prostate
• Species: Mouse

reviewed by: Natalie Erdmann ICC Human 08/24/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: Multiple myeloma cells lines LP-1 and JJN3 cytospins
• Species: Human
• Lot: G-4

reviewed by: Verified Customer IHC-P Human 12/29/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 19582877
• Species: Human

reviewed by: Bryan Tinsley ICC Human 06/09/2014

Summary

• Application: Immunocytochemistry
• Species: Human

reviewed by: Jian Zong WB Mouse 09/30/2010

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Mouse

FAQs for Ki67/MKI67 Antibody (NB500-170). (Showing 1 - 3 of 3 FAQs).

1. Could you please let us know if you have the antibody which may have cross-reactivity to hamster tissue in IHC(paraffin or frozen)? Also, please tell us reference information using your products to hamster tissue.
   • Thank you for your patience. The only information I was able to gain is that the protein has 61% homology with the chinese hamster. Our lab only recommends use if the % homology is 85% or above so I would not recommend our Ki67 antibodies for your customer. However if they are willing to still test this they would become eligable for our Innovators Reward Program in which they would gain a 50% refund and a 50% discount on a future purchase. All you would need to do is email us at innovators@novusbio.com.
2. What is the IF protocol which was used to obtain the IF image on the datasheet?
   • Our lab used our general ICC protocol to obtain the image seen on our website.
3. Is there a clone number for this Ki67 antibody?
   • This antibody is polyclonal, and thus it does not have a clone number.

Bioinformatics

• Gene Symbol: MKI67
• Entrez:
  • 4288(Human)
  • 17345(Mouse)
  • 291234(Rat)
• Uniprot:
  • P46013(Human)
  • Q91VE6(Mouse)