KISS1 Antibody

Western Blot: KISS1 Antibody [NBP1-45672] - Analysis of KiSS-1 expression with Jurkat Cell Lysate.

Product Discontinued

KISS1 Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab286128)
• Immunogen: Synthetic peptide surrounding amino acid 17 of mouse KiSS-1 (NP_839991).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KISS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 4 ug/mL

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 5 mM EDTA, 0.5% BSA, 30% Glycerol
• Preservative: 0.03% Proclin 300
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for KISS1 Antibody

• KiSS-1 metastasis-suppressor
• KiSS1
• KiSS-1
• kisspeptin
• Kisspeptin-1
• malignant melanoma metastasis-suppressor
• metastasis-suppressor KiSS-1
• Metastin
• MGC39258

Background

KiSS-1 gene encodes metastin, also known as kisspeptin or KiSS-1 peptide, which binds to a G protein-coupled receptor GPR54. KiSS-1 is dominantly expressed in brain (e.g. anteroventral periventricular nucleus, AVPV arcuate nucleus, ARC), placenta, and pancreas, etc. Metastin plays an important role in regulating the GnRH secretion in brain and MMP-9 expression in placenta, which is associated with development of puberty and sex maturation (e.g. Idiopathic hypogonadotropic hypogonadism, IHH) as well as placentation (e.g. pre-eclampsia), respectively. Metastin was originally discovered as a tumor metastasis suppressor (e.g. melanoma, breast cancer).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for KISS1 Antibody (NBP1-45672)(5)

Publications using NBP1-45672

• Shen Y, Zhao H, Zhang L Et Al. The roles of DNA methylation and hydroxymethylation at short interspersed nuclear elements in the hypothalamic arcuate nucleus during puberty Molecular Therapy - Nucleic Acids 2021-07-01 [PMID: 34513307] (WB)
• Dees WL, Hiney JK, Srivastava VK Regulation of prepubertal dynorphin secretion in the medial basal hypothalamus of the female rat J. Neuroendocrinol. 2019-12-01 [PMID: 31715027] (WB, Rat)
• Srivastava Vinod K, Hiney Jill K, Dees William L et al. Alcohol Delays the Onset of Puberty in the Female Rat by Altering Key Hypothalamic Events. Alcoholism, Clinical and Experimental Research 2018-07-01 [PMID: 29689132] (WB, Rat)
• Hiney JK, Srivastava VK, Vaden Anderson DN et al. Regulation of kisspeptin synthesis and release in the preoptic/anterior hypothalamic region of prepubertal female rats: actions of IGF-1 and alcohol Alcohol. Clin. Exp. Res. 2017-10-26 [PMID: 29072778] (Rat)
• Srivastava VK, Hiney JK, Dees WL. Manganese stimulated Kisspeptin is mediated by the Insulin-like growth factor-1/Akt/ mammalian target of rapamycin pathway in the prepubertal female rat. Endocrinology 2016-06-16 [PMID: 27309941] (WB)

Bioinformatics

• Gene Symbol: KISS1
• Entrez:
  • 3814(Human)
  • 280827(Mouse)
• Uniprot:
  • Q6Y4S4(Mouse)