Klotho beta Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Klotho beta Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Klotho beta (R&D Systems, Catalog # 2619-KB)
  Phe53-Leu995
  Accession # NP_112457
• Specificity: Detects mouse Klotho beta in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant mouse Klotho is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klotho beta Antibody

• betaKlotho
• beta-klotho
• BKL
• KLB
• klotho beta like
• Klotho beta
• klotho beta-like protein
• MGC142213

Background

Klotho beta , a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (2). The Klotho beta mediated interaction of FGF15 (human FGF19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2 - 4). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2 - 3, 5). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 6). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (7). The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta , respectively. The low identity with rat reflects aa discordance within rodent ECD.

1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
2. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
3. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
4. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
5. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
6. Chang, Q. et al. (2005) Science 310:490.
7. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Klotho beta Antibody (AF2619)(19)

Publications using AF2619

• Kanako Kobayashi, Kazuko Iwasa, Rika Azuma-Suzuki, Takeshi Kawauchi, Yo-ichi Nabeshima Feto-maternal cholesterol transport regulated by beta -Klotho–FGF15 axis is essential for fetal growth Life Science Alliance 2023-10-01 [PMID: 37541847] (Western Blot, Transgenic Mouse)
• Kanako Kobayashi, Kazuko Iwasa, Rika Azuma-Suzuki, Takeshi Kawauchi, Yo-ichi Nabeshima Feto-maternal cholesterol transport regulated by beta -Klotho–FGF15 axis is essential for fetal growth Life Science Alliance 10/1/2023 [PMID: 37541847]
• Shirong Wang, Lin Zha, Xin Cui, Yu‐Te Yeh, Ruochuan Liu, Jia Jing et al. Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation Advanced Science 2023-07-01 [PMID: 37282749] (Western Blot, Mouse, Transgenic Mouse)
• Shirong Wang, Lin Zha, Xin Cui, Yu‐Te Yeh, Ruochuan Liu, Jia Jing et al. Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation Advanced Science 7/1/2023 [PMID: 37282749]
• YK Wu, ZN Ren, SL Zhu, YZ Wu, G Wang, H Zhang, W Chen, Z He, XL Ye, QX Zhai Sulforaphane ameliorates non-alcoholic fatty liver disease in mice by promoting FGF21/FGFR1 signaling pathway Acta pharmacologica Sinica, 2021-10-15;0(0):. 2021-10-15 [PMID: 34654875] (Western Blot, Mouse)
• Lei Ying, Na Li, Zhengyue He, Xueqin Zeng, Yan Nan, Jiantong Chen et al. Fibroblast growth factor 21 Ameliorates diabetes-induced endothelial dysfunction in mouse aorta via activation of the CaMKK2/AMPK alpha signaling pathway Cell Death & Disease 9/11/2019 [PMID: 31511499]
• CA Gilroy, S Roberts, A Chilkoti Fusion of fibroblast growth factor 21 to a thermally responsive biopolymer forms an injectable depot with sustained anti-diabetic action J Control Release, 2018-03-15;0(0):. 2018-03-15 [PMID: 29551712] (Western Blot, Mouse)
• Kathleen R. Markan, Meghan C. Naber, Sarah M. Small, Lila Peltekian, Rachel L. Kessler, Matthew J. Potthoff FGF21 resistance is not mediated by downregulation of beta-klotho expression in white adipose tissue Molecular Metabolism 6/1/2017 [PMID: 28580290]
• LD BonDurant, M Ameka, MC Naber, KR Markan, SO Idiga, MR Acevedo, SA Walsh, DM Ornitz, MJ Potthoff FGF21 Regulates Metabolism Through Adipose-Dependent and -Independent Mechanisms Cell Metab., 2017-04-04;25(4):935-944.e4. 2017-04-04 [PMID: 28380381] (Western Blot, Human)
• Ganesh Kolumam, Mark Z. Chen, Raymond Tong, Jose Zavala-Solorio, Lance Kates, Nicholas van Bruggen et al. Sustained Brown Fat Stimulation and Insulin Sensitization by a Humanized Bispecific Antibody Agonist for Fibroblast Growth Factor Receptor 1/ beta Klotho Complex EBioMedicine 7/1/2015 [PMID: 26288846]
• Ganesh Kolumam, Mark Z. Chen, Raymond Tong, Jose Zavala-Solorio, Lance Kates, Nicholas van Bruggen et al. Sustained Brown Fat Stimulation and Insulin Sensitization by a Humanized Bispecific Antibody Agonist for Fibroblast Growth Factor Receptor 1/ beta Klotho Complex EBioMedicine 2015-07-01 [PMID: 26288846] (Western Blot, Transgenic Mouse)
• Fu T, Seok S, Choi S et al. MiR-34a inhibits beige and brown fat formation in obesity in part by suppressing adipocyte FGF21 signaling and SIRT1 function. Mol Cell Biol. 9/2/2014 [PMID: 25182532]
• Keipert S, Ost M, Johann K, Imber F, Jastroch M, van Schothorst E, Keijer J, Klaus S Skeletal muscle mitochondrial uncoupling drives endocrine cross-talk through the induction of FGF21 as a myokine. Am J Physiol Endocrinol Metab, 2013-12-17;306(5):E469-82. 2013-12-17 [PMID: 24347058] (Western Blot, Mouse)
• Fu T, Choi S, Kim D, Seok S, Suino-Powell K, Xu H, Kemper J Aberrantly elevated microRNA-34a in obesity attenuates hepatic responses to FGF19 by targeting a membrane coreceptor beta-Klotho. Proc Natl Acad Sci U S A, 2012-09-17;109(40):16137-42. 2012-09-17 [PMID: 22988100] (IHC, Western Blot, Mouse)
• Veniant MM, Hale C, Helmering J, Chen MM, Stanislaus S, Busby J, Vonderfecht S, Xu J, Lloyd DJ FGF21 Promotes Metabolic Homeostasis via White Adipose and Leptin in Mice. PLoS ONE, 2012-07-06;7(7):e40164. 2012-07-06 [PMID: 22792234] (Western Blot, Mouse)
• Wu S, Levenson A, Kharitonenkov A, De Luca F Fibroblast Growth Factor 21 (FGF21) Inhibits Chondrocyte Function and Growth Hormone Action Directly at the Growth Plate. J. Biol. Chem., 2012-06-13;287(31):26060-7. 2012-06-13 [PMID: 22696219] (Western Blot, Mouse)
• Gomez IG, Tang J, Wilson CL, Yan W, Heinecke JW, Harlan JM, Raines EW Metalloproteinase-mediated Shedding of Integrin beta2 promotes macrophage efflux from inflammatory sites. J. Biol. Chem., 2011-12-14;287(7):4581-9. 2011-12-14 [PMID: 22170060] (Western Blot, Mouse)
• Micanovic R, Raches DW, Dunbar JD, Driver DA, Bina HA, Dickinson CD, Kharitonenkov A Different roles of N- and C- termini in the functional activity of FGF21. J. Cell. Physiol., 2009-05-01;219(2):227-34. 2009-05-01 [PMID: 19117008] (Western Blot, Mouse)
• Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M BetaKlotho is required for metabolic activity of fibroblast growth factor 21. Proc. Natl. Acad. Sci. U.S.A., 2007-04-23;104(18):7432-7. 2007-04-23 [PMID: 17452648] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: KLB
• Uniprot:
  • NP_112457()