KSR1 Antibody (JE57-75)

Western Blot: KSR1 Antibody (JE57-75) [NBP3-32512] - Western blot analysis of KSR1 on different lysates with Rabbit anti-KSR1 antibody (NBP3-32512) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HT-29 cell lysate Lane 4: HDLM-2 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 120/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32512) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: KSR1 Antibody (JE57-75) [NBP3-32512] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-KSR1 antibody (NBP3-32512) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32512) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: KSR1 Antibody (JE57-75) [NBP3-32512] - Flow cytometric analysis of C6 cells labeling KSR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32512, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: JE57-75
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

KSR1 Antibody (JE57-75) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human KSR1 aa 874-923 / 923. (Uniprot: Q8IVT5)
• Localization: Cytoplasm, Membrane, Cell membrane, Cell projection, ruffle membrane, Endoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KSR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for KSR1 Antibody (JE57-75)

• kinase suppressor of ras 1
• kinase suppressor of ras
• KSRRSU2

Background

Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KSR1