Lactate Dehydrogenase A/LDHA Antibody - BSA Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Increased lactate level detected in the hippocampus of streptozotocin-injected mice fed an HFD (HFD + STZ).b LDHA and LDHB protein levels were measured by western blot assay. c LDHA protein levels were quantified by the ratio to B-actin in the hippocampus. f Cropped images of LDHA and LDHB in the cortex. g Quantified LDHA in the cortex by the ratio to B-actin. Values are expressed as means +/- SEMs (n = 5 for both groups). * p < 0.05. Image collected and cropped by Citeab from the following publication (Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice. Mol Brain (2019) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Immunocytochemical analysis of Lactate Dehydrogenase A in HeLa cells

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Staining of postnatal day 8(P8) Plp-EGFP transgenic mice brain section. Image from verified customer review.

Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - An intracellular stain was performed on HeLa cells with Lactate Dehydrogenase A/LDHA Antibody NBP1-48336AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Analysis of Lactate Dehydrogenase A in HeLa whole cell lysates.

Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Analysis of Lactate Dehydrogenase A in NIH/3T3 whole cell lysates.

Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lactate Dehydrogenase A expression in bovine skeletal muscle lysate. Image from verified customer review.

Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lactate Dehydrogenase A expression in porcine skeletal muscle lysate. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - The effect of the co-culturing of cancer cells and fibroblasts on the expression of Hk2 and Ldha. In the co-cultures, the expression of Ldha was reduced in cancer cells, but was elevated in fibroblasts. Bar = 50 um. The ratio of the fluorescence of Hk2 and Ldha to beta-actin in the MLF monoculture was assumed to be 1. All the experiments were performed in triplicate and obtained similar results, and representative data from one experiment are shown in the figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31947613/) licensed under a CC-BY license.

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Staining of Lactate Dehydrogenase A in lung tissue.

Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336]

Simple Western: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lane view shows a specific band for Lactate dehydrogenase A in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Simple Western: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Lactate Dehydrogenase A/LDHA Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LDHA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA reported in scientific literature (PMID 35052722)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 25004202)
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:50
  • Western Blot 0.5ug/ml
• Application Notes: In Western blot, a band is seen at approx. 36 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.1 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 40 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate
  HeLa Whole Cell Lysate

• Control Peptide:

  Lactate Dehydrogenase A/LDHA Peptide (NBP1-48336PEP)

Reactivity Notes

Porcine reactivity reported in scientific literature (Elgin et al).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody - BSA Free

• Cell proliferation-inducing gene 19 protein
• EC 1.1.1
• EC 1.1.1.27
• GSD11
• Lactate Dehydrogenase A
• lactate dehydrogenase M
• LDH muscle subunit
• LDH1
• LDHA
• LDH-A
• LDHM
• LDH-M
• L-lactate dehydrogenase A chain
• proliferation-inducing gene 19
• Renal carcinoma antigen NY-REN-59

Background

Lactate dehydrogenase (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of NADH and NAD+. Mammalian LDH has five tetrameric isozymes comprising combinations of two subunits (i) A subunit (LDHA or LDHM) predominates in skeletal muscle where it involve in anaerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predominates in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including renal, breast, gastric, nasopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient anaerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several malignancies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertional myoglobinuria and glycogen storage disease type 11 (GSD11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336)(43)

Publications using NBP1-48336

• Cappellesso F, Orban MP, Shirgaonkar N et al. Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer Nature cancer 2022-12-01 [PMID: 36522548] (WB, Mouse)
• Ahmad F, White M, Yamamoto K et al. Metabolic and imaging phenotypes associated withRB1loss in castrate resistant prostate cancer bioRxiv 2023-11-17 (WB, Human)
• Melissa Schwab, Ali Bashiri Dezfouli, Mohammad Khosravi, Bayan Alkotub, Lisa Bauer, Mohammad Javed Tahmasebi Birgani, Gabriele Multhoff The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response. Radiation oncology (London, England) 2024-01-18 [PMID: 38229111]
• Hu Z, Yu X, Ding R et al. Glycolysis drives STING signaling to facilitate dendritic cell antitumor function The Journal of clinical investigation 2023-02-23 [PMID: 36821379] (WB, Mouse)
• Abigail M Maucieri, David H Townson Evaluating the impact of the hexosamine biosynthesis pathway and O-GlcNAcylation on glucose metabolism in bovine granulosa cells. Molecular and cellular endocrinology 2023-02-28 [PMID: 36690170]
• Nsiah NY, Inman DM. Destabilizing COXIV in M�ller Glia Increases Retinal Glycolysis and Alters Scotopic Electroretinogram Cells 2022-11-24 [PMID: 36497016] (Immunohistochemistry-Frozen, Mouse)
• Marwarha G, Rosand O, Slagsvold KH, Hoydal MA GSK3 beta Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia International journal of molecular sciences 2022-08-19 [PMID: 36012628] (S-ELISA, Human)
• Wicks JC Understanding Beef Quality Development and Different Feeding Regimes Thesis 2023-01-01 (WB, Bovine)
  
  Details:
  1:30000 dilution
• Pappenhagen N, Yin E, Morgan AB et al. Stretch stress propels glutamine dependency and glycolysis in optic nerve head astrocytes Frontiers in neuroscience 2022-08-05 [PMID: 35992925] (Simple Western, WB, Rat)
• Schwab M, Multhoff G A Low Membrane Hsp70 Expression in Tumor Cells With Impaired Lactate Metabolism Mediates Radiosensitization by NVP-AUY922 Frontiers in oncology [PMID: 35463341] (WB, Mouse)
• Marwarha G, Rosand o, Scrimgeour N et al. miR-210 Regulates Apoptotic Cell Death during Cellular Hypoxia and Reoxygenation in a Diametrically Opposite Manner Biomedicines 2021-12-25 [PMID: 35052722] (ELISA)
• Schwab M, Thunborg K, Azimzadeh O et al. Targeting Cancer Metabolism Breaks Radioresistance by Impairing the Stress Response Cancers (Basel) 2021-07-27 [PMID: 34359663] (Immunohistochemistry-Frozen, Mouse)
• Shen W, Lee SR, Mathai AE et al. Effect of selectively knocking down key metabolic genes in MUller glia on photoreceptor health Glia 2021-04-09 [PMID: 33835598]
• Pinto IF, Soares RRG, MAkinen ME et al. Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production ACS sensors 2021-03-26 [PMID: 33724791]
• Osis G, Traylor AM, Black LM et al. Expression of Lactate Dehydrogenase A and B isoforms in the mouse kidney American journal of physiology. Renal physiology 2021-03-15 [PMID: 33719570]
• Weiss-Sadan, T, Itzhak, G Et al. Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation. Mol Cell Proteomics 2019-07-01 [PMID: 33451519] (IF/IHC, Human)
• Gizak A, McCubrey JA, Rakus D. Cell-to-cell lactate shuttle operates in heart and is important in age-related heart failure Aging (Albany NY) 2020-02-08 [PMID: 32035422] (Immunohistochemistry-Frozen, Mouse)
• Jo YH Hydrocarboxylic acid receptor 1 in BAT regulates glucose uptake in mice fed a high-fat diet PLoS ONE 2020-01-30 [PMID: 31999787] (WB, Mouse)
• Dang DS Role of Mitochondria in Postmortem Proteolysis and Meat Tenderness Thesis 2022-01-01 [PMID: 31935569] (WB, Bovine)
• Rimmer L Investigating the metabolic changes that accompany skeletal muscle hypertrophy Thesis 2023-01-01 (Western Blot)
• Knowles HJ Distinct roles for the hypoxia-inducible transcription factors HIF-1alpha and HIF-2alpha in human osteoclast formation and function Scientific reports 2020-12-03 [PMID: 33273561] (WB, Human)
• Dang DS, Buhler JF, Thornton KJ et al. Myosin heavy chain isoform and metabolic profile differ in beef steaks varying in tenderness Meat Sci. 2020-07-28 [PMID: 32739757] (WB, Bovine)
• Duda P, Janczara J et al. The Reverse Warburg Effect is Associated with Fbp2-Dependent Hif1 alpha Regulation in Cancer Cells Stimulated by Fibroblasts. Cells 2020-01-14 [PMID: 31947613] (ICC/IF, Mouse)
• Apaoblaza A, Gerrard SD, Matarneh SK et al. Muscle from grass- and grain-fed cattle differs energetically Meat Sci. 2019-11-06 [PMID: 31734468] (WB, Bovine)
• Kaushik DK, Bhattacharya A, Mirzaei R et al. Enhanced glycolytic metabolism supports transmigration of brain-infiltrating macrophages in multiple sclerosis J. Clin. Invest. 2019-05-21 [PMID: 31112527] (WB, Mouse)
• Weiss Sadan, T;Itzhak, G;Kaschani, F;Yu, Z;Mahameed, M;Anaki, A;Ben-Nun, Y;Merquiol, E;Tirosh, B;Kessler, B;Kaiser, M;Blum, G; Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation Mol. Cell Proteomics 2019-04-22 [PMID: 31010818] (WB, Human)
• Rimmer L, Geisbrecht E, Chao M et al. Investigating the metabolic changes that accompany skeletal muscle maturation Physiology 2023-05-01
• Schwab M, Dezfouli A, Khosravi M et al. The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response Research Square 2023-03-20 (WB)
• Choi YS, Song JE, Lee JE et al. Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice Mol Brain 2019-11-01 [PMID: 31675964] (WB, Mouse)
• Xu Q, Li X et al. Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation1. J Anim Sci 2019-02-07 [PMID: 31267132] (WB, Bovine)
• Boyle J, Patronas NJ, Smirniotopoulos J et al CRH stimulation improves 18F-FDG-PET detection of pituitary adenomas in Cushing's disease. Endocrine. 2019-07-01 [PMID: 31062234] (IHC-P, Human)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Xu, Q;Li, X;Ma, L;Loor, JJ;Coleman, DN;Jia, H;Liu, G;Xu, C;Wang, Y;Li, X; Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation J. Anim. Sci. 2019-04-15 [PMID: 30985866] (Bovine)
• Dodo M, Kitamura H, Shima H et al. Lactate Dehydrogenase C Is Required for the Protein Expression of a Sperm-specific Isoform of Lactate Dehydrogenase A J. Biochem. 2018-12-24 [PMID: 30590713] (WB, Mouse)
• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
• Matsumoto S, Kishimoto S, Saito K et al. Metabolic and physiologic imaging biomarkers of the tumor microenvironment predict treatment outcome with radiation or a hypoxia-activated prodrug in mice Cancer Res. 2018-05-23 [PMID: 29792309] (Immunohistochemistry-Frozen, Mouse)
• Jason D Yang, Daniel Mott, Rujapak Sutiwisesak, Yu-Jung Lu, Fiona Raso, Britni Stowell, Greg Hunter Babunovic, Jinhee Lee, Steve M Carpenter, Sing Sing Way, Sarah M Fortune, Samuel M Behar Mycobacterium tuberculosis-specific CD4+ and CD8+ T cells differ in their capacity to recognize infected macrophages. PLoS pathogens 2018-07-06 [PMID: 29782535]
• Hulley PA, Bishop T, Vernet A et al. Hypoxia-Inducible Factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2. J. Pathol. 2017-04-18 [PMID: 28418093]
• Elgin JM Determining the underlying factors of fresh ham color variation Meat Sci 2017-02-19 [PMID: 28214148]
• Takenouchi T, Tsukimoto M, Iwamaru Y et al. Extracellular ATP induces unconventional release of glyceraldehyde-3-phosphate dehydrogenase from microglial cells. Immunol. Lett. 2015-08-12 [PMID: 26277554] (WB, Mouse)
  
  Details:
  Lactate Dehydrogenase A/LDHA Antibody was used for WB on microvesicle and exosome enriched /depleted fractions from LPS-primed MG6 cells (mouse microglial cell line immortalized by a replication-deficient retroviral vector containing human c-myc gene) after ATP stimulation (Fig. 3A).
• Kim J, Han J, Jang Y et al. High-capacity glycolytic and mitochondrial oxidative metabolisms mediate the growth ability of glioblastoma Int. J. Oncol. 2015-07-21 [PMID: 26202438]
• Pastor-Soler NM, Sutton TA, Mang HE et al. Muc1 is protective during kidney ischemia-reperfusion injury Am J Physiol Renal Physiol. 2015-04-28 [PMID: 25925251] (WB, Mouse)
• Adochite RC, Moshnikova A, Carlin SD et al. Targeting Breast Tumors with pH (Low) Insertion Peptides. Mol. Pharm. 2014-07-08 [PMID: 25004202] (IHC-Fr, Mouse)
  
  Details:
  Lactate Dehydrogenase A antibody used at 1:100 dilution for IHC-Fr application on 4% PFA fixed cryosections of FVB/N-Tg(MMTV-PyVT)634Mul/J transgenic female mice (develops palpable mammary tumors at 12-15 wk of age) as well as tissues from control noncarrier FVB/NJ female mice (Figures 6 and 7). IHC-Fr of LDHA also performed on the cryosections of tumors that were generated by grafting 4T1 mouse mammary tumor cell line on BALB/c mice.

Reviews for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336) (5)

Average Rating: 4.4

(Based on 5 reviews)

We have 5 reviews tested in 3 species: Human, Mouse, Other.

reviewed by: Verified Customer WB Human 10/09/2023

Summary

• Application: Western Blot
• Sample Tested: glioblastoma cell lysates
• Species: Human
• Lot: A-4

reviewed by: Verified Customer WB Human 03/10/2020

Summary

• Application: Western Blot
• Sample Tested: HepG2 human hepatocellular carcinoma cell line,Huh-7 human hepatoma cell line
• Species: Human
• Lot: b-3

Comments

• Comments: Used it in 1:1000 with milk TBST.

reviewed by: Eric England WB Other 10/10/2014

Summary

• Application: Western Blot
• Sample Tested: Whole Skeletal Muscle Lysate
• Species: Other
• Lot: A

reviewed by: Eric England WB Other 10/01/2014

Summary

• Application: Western Blot
• Sample Tested: Whole Skeletal Muscle Lysate
• Species: Other
• Lot: A

reviewed by: Bryan Tinsley IHC Mouse 03/17/2014

Summary

• Application: Immunohistochemistry
• Species: Mouse

Bioinformatics

• Gene Symbol: LDHA
• Entrez:
  • 3939(Human)
  • 16828(Mouse)
• Uniprot:
  • P00338(Human)