Laminin gamma 1 Antibody (CL3201) - BSA Free

Western Blot: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Analysis in human lung tissue.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Staining of human tonsil shows absence of immunoreactivity in lymphoid cells (negative control).

Western Blot: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Analysis of purified human recombinant Laminin-111, Laminin-121, Laminin-211, Laminin-221, Laminin-411, Laminin-421, Laminin-511, Laminin-521 and Laminin-332.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Staining of human stomach shows strong immunoreactivity in basement membrane of glandular epithelium.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Staining of human endometrium shows strong positivity in basement membrane of glandular epithelium.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Staining of human kidney shows immunoreactivity in basement membrane of renal tubules and membranous positivity in glomeruli.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Staining of human endometrium shows strong positivity in basement membrane of glandular epithelium.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Staining of human cortex shows strong positivity in endothelium.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3201) [NBP2-42397] - Staining of human testis shows immunoreactivity in basement membrane of seminiferous tubules.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL3201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Laminin gamma 1 Antibody (CL3201) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P11047, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (86%), Rat (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Laminin gamma 1 Antibody (CL3201) - BSA Free

• LAMB2Laminin-7 subunit gamma
• LAMC1
• Laminin B2 chain
• Laminin B2
• Laminin gamma 1
• laminin subunit gamma-1
• laminin, gamma 1 (formerly LAMB2)
• Laminin-1 subunit gamma
• Laminin-10 subunit gamma
• Laminin-11 subunit gamma
• Laminin-2 subunit gamma
• Laminin-3 subunit gamma
• Laminin-4 subunit gamma
• Laminin-6 subunit gamma
• Laminin-8 subunit gamma
• Laminin-9 subunit gamma
• MGC87297
• S-LAM gamma
• S-laminin subunit gamma

Background

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMC1
• Uniprot:
  • P11047(Human)