Leptin R Antibody - BSA Free

Western Blot: Leptin R Antibody [NB120-5593] - Analysis of both short and long forms of OB-R from mouse lung tissue extract.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 10 mg/ml

Leptin R Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R.
• Localization: Type I membrane protein. Except for form E which could be soluble.
• Specificity: Leptin Receptor
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LEPR
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Application Notes: WB: Detects bands of approx. 100 and 125 kDa, corresponding to the long and short forms (respectively) of leptin receptor OB-R from mouse brain extract. Two smaller bands of approx. 31 and 35 kDa are also detected, which cannot be competed out with immunogenic peptide.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9421392). Rat reactivity reported in scientific literature (PMID: 12810558).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 10 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Leptin R Antibody - BSA Free

• B219
• CD295 antigen
• CD295
• DB
• DKFZp686B1731
• huB219
• LEPR
• LEP-R
• Leptin R
• leptin receptor
• LeptinR
• OB R
• OB receptor
• OB-R
• OBRCD295

Background

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin R Antibody (NB120-5593)(5)

Publications using NB120-5593

• Fang Z, Fan M, Yuan D et al. Downregulation of hepatic lncRNA Gm19619 improves gluconeogenesis and lipogenesis following vertical sleeve gastrectomy in mice Communications Biology 2023-01-27 [PMID: 36707678] (Western Blot, Mouse)
• Drori A, Gammal A, Azar S et al. CB1R Regulates Soluble Leptin Receptor Levels via CHOP, Contributing to Hepatic Leptin Resistance Elife 2020-11-19 [PMID: 33210603]
• R Yamada, S Odamaki, M Araki, T Watanabe, K Matsuo, K Uchida, T Kato, Y Ozaki-Masu, A Takenaka Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents PLoS ONE, 2019-07-15;14(7):e0219603. 2019-07-15 [PMID: 31306448] (WB, Mouse, Rat)
• Lamming DW, Demirkan G, Boylan JM et al. Hepatic signaling by the mechanistic target of rapamycin complex 2 (mTORC2). FASEB J. 2013-09-27 [PMID: 24072782] (WB, Mouse)
• Liu E, Samad F, Mueller BM. Local adipocytes enable estrogen-dependent breast cancer growth: Role of leptin and aromatase. Adipocyte. 2013-07-01 [PMID: 23991363] (WB, Mouse)

Bioinformatics

• Gene Symbol: LEPR
• Entrez:
  • 3953(Human)
  • 16847(Mouse)
  • 24536(Rat)
• Uniprot:
  • P48357()