![Western Blot: LexA DNA Binding Region Antibody [NB600-1137] - LexA-pRB transfected yeast cell extract probed with anti-LexA at 0.1ug/ml.](http://images.novusbio.com/fullsize/LexA-DNA-Binding-Region-Antibody-Western-Blot-NB600-1137-img0002.jpg "Western Blot: LexA DNA Binding Region Antibody [NB600-1137] - LexA-pRB transfected yeast cell extract probed with anti-LexA at 0.1ug/ml.")
| Reactivity | BaSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | His-tagged DNA binding domain of LexA protein corresponding to amino acid residues 1-202. |
| Localization | Nuclear |
| Specificity | Recognizes the LexA portion of bait fusion proteins used in the yeast two-hybrid system. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH 7.4) and 0.15M NaCl |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
| Publications using NB600-1137 | Applications | Species |
|---|---|---|
| Mai1 protein acts between host recognition of pathogen effectors and MAPK signaling Hind SR, Roberts R, Pedley KF Mol Plant Microbe Interact [PMID: 31251114] (WB, Plant) | WB | Plant |
| Keller R, Schleppi N, Weikum J, Schneider D. Mutational analyses of YqjA, a Tvp38/DedA protein of E. coli FEBS Lett. 2015-03-24 [PMID: 25728272] (WB, Bacteria) | WB | Bacteria |
| Van Alstine S THE SOS RESPONSE IN ESCHERICHIA COLI K12: AN EXPLORATION OF MUTATIONS IN LEXA AND RECA USING FLUORESCENCE MICROSCOPY Thesis 2022-01-01 (WB, E. coli) | WB | E. coli |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")