LIM1 Antibody

Western Blot: LIM1 Antibody [NBP3-12886] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 antibody (NBP3-12886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. LIM1 antibody (NBP3-12886) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Frozen: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LIM1 protein stained by LIM1 antibody (NBP3-12886) diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: LIM1 protein stained by LIM1 antibody (NBP3-12886) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [GT1876] (NBP2-19304) diluted at 1:250. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.LIM1 stained by LIM1 antibody (NBP3-12886) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: LIM1 Antibody [NBP3-12886] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 antibody (NBP3-12886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.LIM1 stained by LIM1 antibody (NBP3-12886) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LIM1 Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human LIM1. The exact sequence is proprietary.
• Predicted Species: Chimpanzee (100%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LHX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Xenopus laevis (88%), Chicken (84%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for LIM1 Antibody

• hLim-1
• Homeobox protein Lim-1
• LHX1
• LIM homeobox 1
• LIM homeobox protein 1
• LIM1
• LIM-1LIM/homeobox protein Lhx1
• LIM1MGC138141
• MGC126723

Background

The LIM1 gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development o

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LHX1