LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free

Western Blot: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S LMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Pr

Immunocytochemistry/Immunofluorescence: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 20S LMP2.

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

Flow Cytometry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Analysis of Jurkat cells, using anti-Proteasome 20S LMP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Analysis of extracts (10ug) from Jurkat cell line by using anti-PSMB9 monoclonal antibody (1:200).

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Human tonsil.

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

Immunohistochemistry: LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free [NBP2-71261] - Staining of paraffin-embedded Human prostate tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 21-219 of human PSMB9(NP_002791) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMB9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 23.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for LMP2/PSMB9 Antibody (OTI1D1) - Azide and BSA Free

• beta1i
• LMP2
• LMP2MGC70470
• Low molecular mass protein 2
• Macropain chain 7
• Multicatalytic endopeptidase complex chain 7
• proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalpeptidase 2)
• proteasome catalytic subunit 1i
• Proteasome chain 7
• proteasome subunit beta 6i
• proteasome subunit beta type-9
• Proteasome subunit beta-1i
• proteasome-related gene 2
• PSMB6iproteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalprotease 2)
• PSMB9
• Really interesting new gene 12 protein
• RING12
• RING12EC 3.4.25.1

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. LMP2 is a catalytic subunit of the 20S proteasome and, upon interferon gamma-induction, replaces the delta subunit. LMP2 alters the specificity of the 20S proteasome and is critical for the production of MHC class I ligands, production of T-lymphocytes, and is suggested to increase the efficiency of antigen presentation of the immune response. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMB9