Human Luteinizing Hormone ELISA Kit (Colorimetric)

Human Luteinizing Hormone ELISA Kit (Colorimetric) [NBP2-60087] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 1 mIU/mL - 50 mIU/mL
• Sensitivity: 0.5 mIU/mL

Human Luteinizing Hormone ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: The minimum detectable dose of human LH is typically less than 0.5 mIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human LH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Standard Curve Range: 1 mIU/mL - 50 mIU/mL
• Sensitivity: 0.5 mIU/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LHB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Luteinizing Hormone ELISA Kit (Colorimetric)

• CGB4;HH23;LHB;LSH-B;LSH-beta;Lutropin subunit beta

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for LH. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LHB