Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Total cell extracts of wild-type (RPE1WT) and KDM3A gene-edited RPE1 mutants (KDM3ACr22, named herein KDM3Anull) immunoblotted with KDM3A, revealing KDM3A-null clones (1, 2, and 8). Image collected and cropped by CiteAb from the following publication (//rupress.org/jcb/article/216/4/999/52171/KDM3A-coordinates-actin-dynamics-with) licensed under a CC-BY license.

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Detection of Human JMJD1A/JHDM2A on HeLa whole cell lysate using NB100-77282. Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A antibody NB100-77281.

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Human cancer cells stained with NB100-77282 and Alexa Fluor 488 conjugated goat-anti-rabbit secondary antibody. DNA stained with Hoechst 33342. Staining shows KDM3A protein in mixed nuclear and cytoplasmic location. ICC/IF image submitted by a verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, ChIP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1271 and 1321 of human jumonji domain containing 1A (JmjC domain-containing histone demethylation protein 2A) using the numbering given in entry NP_060903.2 (GeneID 55818).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Knockout Validated
  • Western Blot 1:2000 - 1:10000
• Application Notes: Use in chromatin immunoprecipitation reported in scientific literature (PMID: 19624751). Use in Immunohistochemistry reported in scientific literature (PMID: 30405805). This Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody is validated for ICC/IF from a verified customer review.
• Control:

  Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24554764).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

• EC 1.14.11
• EC 1.14.11.-
• JHDM2A
• JHDM2AJMJD1AJHMD2A
• JmjC domain-containing histone demethylation protein 2A
• JMJD1
• JMJD1A
• jumonji C domain-containing histone demethylase 2A
• jumonji domain containing 1
• jumonji domain containing 1A
• Jumonji domain-containing protein 1A
• KDM3A
• KIAA0742
• KIAA0742DKFZp686A24246
• Lysine (K)specific Demethylase 3A
• Lysine (K)-specific Demethylase 3A
• lysine-specific demethylase 3A
• testis-specific protein A
• TSGA
• TSGADKFZp686P07111

Background

This gene encodes a zinc finger protein that contains a jumonji domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NB100-77282)(12)

Publications using NB100-77282

• Sui Y, Jiang H, Kellogg CM et al. Promotion of colorectal cancer by transcription factor BHLHE40 involves upregulation of ADAM19 and KLF7 Frontiers in oncology 2023-02-20 [PMID: 36890812] (WB, Human)
• Yuan Sui, Xiaomeng Li, Sangphil Oh, Bin Zhang, Willard M. Freeman, Sook Shin, Ralf Janknecht Opposite Roles of the JMJD1A Interaction Partners MDFI and MDFIC in Colorectal Cancer Scientific Reports 2020-05-26 [PMID: 32457453]
• Kim J, Choi Sa, Kim J et Al. Lysine-specific demethylase 3A is important for autophagic occurrence Biochem. Biophys. Res. Commun. 2020-03-19 [PMID: 32201075] (Human)
• Li X, Oh S, Song H et al. A potential common role of the Jumonji C domain containing 1A histone demethylase and chromatin remodeler ATRX in promoting colon cancer. Oncol Lett 2018-09-24 [PMID: 30405805] (IF/IHC, WB, Human)
• Yeyati PL, Schiller R, Mali G et al. KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability. J. Cell Biol. 2017-02-28 [PMID: 28246120] (WB, IP, Human)
• Ueda J, Ho JC, Lee KL et al. The Hypoxia-Inducible Epigenetic Regulators Jmjd1a and G9a Provide a Mechanistic Link between Angiogenesis and Tumor Growth. Mol. Cell. Biol. 2014-07-28 [PMID: 25071150] (WB, Mouse)
  
  Details:
  JMJD1A antibody used in WB on wild-type (WT) and Jmjd1a knockout (KO1 and KO2) embryonic stem cells (Fig 2B), 1% hypoxia exposed WT ES cells (Fig 4A), WT or Jmjd1a KO ES allograft tumors (8D) and Jmjd1a KO ES cells stably transfected with G9a shRNA (Fig 10A).
• Kasioulis I, Syred HM, Tate P et al. Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis. Mol. Biol. Cell. 2014-02-19 [PMID: 24554764] (WB, Mouse)
• Javierre B, Rodriguez-Ubreva J, Al-Shahrour F et al. Long-Range Epigenetic Silencing Associates with Deregulation of Ikaros Targets in Colorectal Cancer Cells. Mol Cancer Res. 2011-07-07 [PMID: 21737484]
• Inagaki T, Tachibana M, Magoori K et al. Obesity and metabolic syndrome in histone demethylase JHDM2a-deficient mice. Genes Cells. 2009-08-01 [PMID: 19624751] (Chemotaxis, WB, Mouse)
• Yuan SGS Epigenetic Reprogramming in Tumor Plasticity Thesis 2020-01-01 (IP)
• Oh, S, Song, H Et al. Cooperation between ETS transcription factor ETV1 and histone demethylase JMJD1A in colorectal cancer. Int J Oncol 2020-12-01 [PMID: 33174020] (FLOW, Rat)
• Cadot B, Brunetti M, Coppari S et al. Loss of Histone Deacetylase 4 Causes Segregation Defects during Mitosis of p53-Deficient Human Tumor Cells. Cancer Res 69(15):6074-6082. 2009-01-01 [PMID: 19622775]

Review for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NB100-77282) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Indirect Immunofluorescence Human 04/27/2021

Summary

• Application: Other
• Sample Tested: Cancer Cells
• Species: Human
• Lot: A1

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

FAQs for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NB100-77282). (Showing 1 - 1 of 1 FAQs).

1. Would you please let me know if NB100-77282 is validated for ChIP?
   • NB100-77282 hasn't been tested for use in ChIP. Theoretically this antibody can work for ChIP if you get successful cross-linking of JMJD1A to K9 on H3. There should be enough JMJD1A present to be able to capture the quick de-methylation step and you'd only need a couple DNA strands to IP to be able to amplify by PCR. This antibody should work for ChIP but it will most likely IP a low amount of DNA and we can not guarantee it for use in ChIP.

Bioinformatics

• Gene Symbol: KDM3A
• Entrez:
  • 55818(Human)
• Uniprot:
  • Q9Y4C1()