LYVE-1 Antibody (ALY7) - BSA Free

Immunohistochemistry: LYVE-1 Antibody (ALY7) [NBP1-43411] - Immunohistochemistry of cryosections of mouse intestine at 2.5 ug/ml of anti-mouse LYVE-1 antibody followed by Anti-Rat IgG Rhodamine (right). Phase image of same field (left).

Flow Cytometry: LYVE-1 Antibody (ALY7) [NBP1-43411] - Analysis using the Biotin conjugate of NBP1-43411. Staining of LYVE-1 transfected cells with 0.06 ug of Rat IgG1 k Isotype Control Biotin (blue histogram) or 0.06 ug of Anti-Mouse Lyve-1 Biotin (purple histogram) followed by Streptavidin PE.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (ALY7) - BSA Free [NBP1-43411] - Ang II treatment promotes lymphatic marker expression of LECs in vitro. (A) Immunofluorescence staining of LECs with anti-LYVE-1 & anti-VEGFR-3 antibodies. (B) LECs were treated with Ang II (500 nM) for 12 & 24 h, the mRNA levels of LYVE-1, VEGFR-3 & Prox1 were detected by qPCR, & the data were normalized using the reference gene GAPDH (n = 6). (C) The protein expression level of VEGFR-3 was measured by immunoblotting & normalized using beta -actin (n = 4). The results are expressed as the means ± SD, & n represents the number of independent experiments. *P < 0.05, **P < 0.01, & ***P < 0.001 versus Control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33013481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (ALY7) - BSA Free [NBP1-43411] - Ang II infusion increases cardiac lymphangiogenesis via AT1R in vivo. (A) Wild-type mice were subcutaneously infused with Ang II (1,000 ng/kg/min) with or without losartan (10 mg/kg) for 1 or 2 weeks, & the systolic blood pressure was measured by the tail-cuff method (n = 6). (B) Cardiac mRNA expression level of LYVE-1 was measured by qPCR analysis (n = 3). (C) Cardiac mRNA expression level of VEGFR-3 was measured by qPCR analysis (n = 3). (D) Immunofluorescence staining of hearts with anti-LYVE-1 (Red) & anti- VEGFR-3 (Green) antibodies & DAPI (Blue) (Left, n = 6), & the quantification of the LYVE-1+ & Prox1+ lymphatic vessels in the hearts (Right, n = 6). The results are expressed as the means ± SD, & n represents the number of independent experiments. *P < 0.05, **P < 0.01, & ***P < 0.001 versus Sham group; #P < 0.05, ##P < 0.01, & ###P < 0.001 versus Ang II 1W + Saline group; $$$P < 0.001 versus Ang II 2W + Saline group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33013481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (ALY7) - BSA Free [NBP1-43411] - Molecular characterization of LECs in the SCS ceiling with RNA FISH.(A-B) Expression of new cLEC/cluster 2 marker genes Ackr3 (A) & Btnl9 (B) by RNA sequencing (left panels) & RNA FISH (right panels). As GFP fluorescence is lost during tissue processing for RNA FISH, immunofluorescence staining for ANXA2 (red) & LYVE1 (green) served as markers for cLECs & fLECs, respectively. Arrows point to cLECs expressing Ackr3 & Btnl9 transcripts (white). ACKR3, atypical chemokine receptor 3; ANXA2, annexin A2; Btnl9, butyrophilin like 9; cLEC, ceiling LEC; FISH, fluorescence in situ hybridization; fLEC, floor-lining LEC; LEC, lymphatic endothelial cell; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1; SCS, subcapsular sinus; UMAP, Uniform Manifold Approximation & Projection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32251437), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (ALY7) - BSA Free [NBP1-43411] - Molecular characterization of LECs in the SCS ceiling with immunofluorescence staining.(A–C) Expression of new cLEC/cluster 2 marker genes ANXA2 (A), FABP4 (B), & CD36 (C) by RNA sequencing (left panels) & immunofluorescence staining (right panels) in Ackr4-GFP reporter mice. GFP (white) & immunofluorescence costaining for LYVE1 (green) served as markers for cLECs & fLECs, respectively. ACKR4, atypical chemokine receptor 4; ANXA2, annexin A2; CD, cluster of differentiation; cLEC, ceiling LEC; FABP4, fatty acid binding protein 4; fLEC, floor-lining LEC; GFP, green fluorescent protein; LEC, lymphatic endothelial cell; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1; SCS, subcapsular sinus; UMAP, Uniform Manifold Approximation & Projection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32251437), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (ALY7) - BSA Free [NBP1-43411] - Molecular characterization of LECs in the SCS ceiling with immunofluorescence staining.(A–C) Expression of new cLEC/cluster 2 marker genes ANXA2 (A), FABP4 (B), & CD36 (C) by RNA sequencing (left panels) & immunofluorescence staining (right panels) in Ackr4-GFP reporter mice. GFP (white) & immunofluorescence costaining for LYVE1 (green) served as markers for cLECs & fLECs, respectively. ACKR4, atypical chemokine receptor 4; ANXA2, annexin A2; CD, cluster of differentiation; cLEC, ceiling LEC; FABP4, fatty acid binding protein 4; fLEC, floor-lining LEC; GFP, green fluorescent protein; LEC, lymphatic endothelial cell; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1; SCS, subcapsular sinus; UMAP, Uniform Manifold Approximation & Projection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32251437), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (ALY7) - BSA Free [NBP1-43411] - Molecular characterization of LECs in the SCS ceiling with immunofluorescence staining.(A–C) Expression of new cLEC/cluster 2 marker genes ANXA2 (A), FABP4 (B), & CD36 (C) by RNA sequencing (left panels) & immunofluorescence staining (right panels) in Ackr4-GFP reporter mice. GFP (white) & immunofluorescence costaining for LYVE1 (green) served as markers for cLECs & fLECs, respectively. ACKR4, atypical chemokine receptor 4; ANXA2, annexin A2; CD, cluster of differentiation; cLEC, ceiling LEC; FABP4, fatty acid binding protein 4; fLEC, floor-lining LEC; GFP, green fluorescent protein; LEC, lymphatic endothelial cell; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1; SCS, subcapsular sinus; UMAP, Uniform Manifold Approximation & Projection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32251437), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (ALY7) - BSA Free [NBP1-43411] - Molecular characterization of LECs in the SCS ceiling with RNA FISH.(A-B) Expression of new cLEC/cluster 2 marker genes Ackr3 (A) & Btnl9 (B) by RNA sequencing (left panels) & RNA FISH (right panels). As GFP fluorescence is lost during tissue processing for RNA FISH, immunofluorescence staining for ANXA2 (red) & LYVE1 (green) served as markers for cLECs & fLECs, respectively. Arrows point to cLECs expressing Ackr3 & Btnl9 transcripts (white). ACKR3, atypical chemokine receptor 3; ANXA2, annexin A2; Btnl9, butyrophilin like 9; cLEC, ceiling LEC; FISH, fluorescence in situ hybridization; fLEC, floor-lining LEC; LEC, lymphatic endothelial cell; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1; SCS, subcapsular sinus; UMAP, Uniform Manifold Approximation & Projection. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32251437), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC
• Clone: ALY7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

LYVE-1 Antibody (ALY7) - BSA Free Summary

• Immunogen: This LYVE-1 Antibody (ALY7) was developed against mouse LYVE1.
• Marker: Lymphatic Vessel Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: LYVE1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.125 ug/10^6 cells in 100 uL
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 2.5 ug/mL
• Application Notes: This ALY7 antibody has been tested by flow cytometric analysis of transfected cell line or immunofluorescent microscopy of cryosections of mouse intestine. This can be used at less than or equal to 0.125 ug per million cells in a 100 uL total staining volume for flow cytometry and 2.5 ug/mL for immunofluorescent micoscopy. Use in Immunocytochemistry/Immunofluorescence was reported in scientific literature.
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID: 33783987).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for LYVE-1 Antibody (ALY7) - BSA Free

• cell surface retention sequence binding protein-1
• Cell surface retention sequence-binding protein 1
• CRSBP1
• CRSBP-1
• extracellular link domain containing 1
• extracellular link domain-containing 1
• Extracellular link domain-containing protein 1
• HAR
• Hyaluronic acid receptor
• lymphatic vessel endothelial hyaluronan receptor 1
• lymphatic vessel endothelial hyaluronic acid receptor 1
• LYVE1
• LYVE-1
• UNQ230/PRO263
• XLKD1

Background

LYVE-1 (Lymphatic Vessel Endothelial Receptor 1) is a receptor for the extracellular matrix mucopolysaccharide hyaluronan (HA) and is primarily expressed by lymphatic endothelial cells in addition to the sinusoidal endothelium of the liver and spleen (1-3). HA, also called hyaluronic acid, is a main component of the extracellular matrix and functions largely in cell adhesion, migration, and tissue remodeling (2). HA undergoes a turnover process that involves release from the tissues to the afferent lymph, degradation within the lymph nodes, and removal of fragments by the liver (2,3). LYVE-1, in addition to other lymphatic proteins including VEGFR3, Prox1, and podoplanin, is a common marker for differentiating between the blood and lymphatic systems (2,3). Furthermore, LYVE-1 is closely related to the leukocyte receptor CD44, having ~44% sequence similarity (1-3). Like CD44, the LYVE-1 protein contains an extracellular HA-binding link domain, with N- and C-terminal extensions, located at the end of a glycosylated juxtamembrane domain stalk region, followed by a transmembrane region, and a cytoplasmic tail (1-3). The key features of the HA-binding like molecule are the three disulfide bridges formed by six cysteine residues (1-3). LYVE-1 is synthesized as a protein of 322 amino acids in length with a theoretical molecular weight of 35 kDa, although due to O-glycosylation often appears in SDS-PAGE at a molecular weight ranging from ~60-70 kDa (2,4). The role of HA-binding is further elucidated by the identification of LYVE-1 as a docking receptor for dendritic cells and macrophages, binding their surface HA to control the entry and migration into lymph vessels (1).

LYVE-1 has been an important marker in studies of embryonic and tumor lymphangiogenesis, as many cancers are characterized by early metastasis to the lymph nodes (1-3, 5). One study of five different vascular tumors in infants used immunohistochemical analysis and found positive LYVE-1 expression in infantile hemangioma, tufted angioma, and kaposiform hemangioendothelioma (5). LYVE-1 along with other markers such as GLUT-1, CD31, CD34, Prox-1, and WT-1 can be used to help provide immunohistologic profiles of various tumors and, when used in conjunction with clinical and histopathologic approaches, may offer better overall diagnosis and disease treatment (5).

References

1. Jackson D. G. (2019). Hyaluronan in the lymphatics: The key role of the hyaluronan receptor LYVE-1 in leucocyte trafficking. Matrix Biology : Journal of the International Society for Matrix Biology. https://doi.org/10.1016/j.matbio.2018.02.001

2. Jackson D. G. (2004). Biology of the lymphatic marker LYVE-1 and applications in research into lymphatic trafficking and lymphangiogenesis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. https://doi.org/10.1111/j.1600-0463.2004.apm11207-0811.x

3. Jackson D. G. (2003). The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends in Cardiovascular Medicine. https://doi.org/10.1016/s1050-1738(02)00189-5

4. Unitprot (Q9Y5Y7)

5. Johnson, E. F., Davis, D. M., Tollefson, M. M., Fritchie, K., & Gibson, L. E. (2018). Vascular Tumors in Infants: Case Report and Review of Clinical, Histopathologic, and Immunohistochemical Characteristics of Infantile Hemangioma, Pyogenic Granuloma, Noninvoluting Congenital Hemangioma, Tufted Angioma, and Kaposiform Hemangioendothelioma. The American Journal of Dermatopathology. https://doi.org/10.1097/DAD.0000000000000983

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LYVE-1 Antibody (NBP1-43411)(24)

Publications using NBP1-43411

• Sin SH, Eason AB, Kim Y, Schneider JW et Al. The complete Kaposi sarcoma-associated herpesvirus genome induces early-onset, metastatic angiosarcoma in transgenic mice Cell Host Microbe 2024-04-23 [PMID: 38653242]
• Semyachkina-Glushkovskaya O, Shirokov A, Blokhina I et al. Intranasal Delivery of Liposomes to Glioblastoma by Photostimulation of the Lymphatic System Pharmaceutics 2022-12-22 [PMID: 36678667] (Immunohistochemistry)
• Caratti G, Desgeorges T, Juban G et al. Macrophagic AMPK alpha 1 orchestrates regenerative inflammation induced by glucocorticoids EMBO reports 2022-12-15 [PMID: 36520372] (FLOW, Mouse)
• Garnier L, Pick R, Montorfani J et al. IFN-gamma-dependent tumor-antigen cross-presentation by lymphatic endothelial cells promotes their killing by T cells and inhibits metastasis Science advances 2022-06-10 [PMID: 35675399] (IF/IHC, Mouse)
• Bai J, Yin L, Yu WJ et al. Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms Oxidative medicine and cellular longevity 2022-02-18 [PMID: 35222798] (IHC-Fr, Mouse)
• Lin Q, Zhang Y, Bai J et al. VEGF C/VEGFR 3 axis protects against pressure overload induced cardiac dysfunction through regulation of lymphangiogenesis Clinical and translational medicine 2021-03-01 [PMID: 33783987] (Mouse)
• Cahill T, Sun X, Ravaud C et al. Tissue-resident macrophages regulate lymphatic vessel growth and patterning in the developing heart Development 2021-01-19 [PMID: 33462113]
• Lin, Q Y, Bai, J Et al. Angiotensin II Stimulates the Proliferation and Migration of Lymphatic Endothelial Cells Through Angiotensin Type 1 Receptors. Front Physiol 2020-10-06 [PMID: 33013481] (WB, Mouse)
• Fujimoto N, He Y, D'Addio M et al. Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes PLoS Biol. 2020-04-01 [PMID: 32251437] (IF/IHC, Mouse)
• Maeda-Smithies N, Hiller S, Dong S et al. Ectopic expression of the Stabilin2 gene triggered by an intracisternal A particle (IAP) element in DBA/2J strain of mice Mamm. Genome 2020-01-07 [PMID: 31912264] (IHC-Fr, Mouse)
• Kang B, Alvarado LJ, Kim T et al. Commensal microbiota drive the functional diversification of colon macrophages Mucosal Immunol. 2019-11-26 [PMID: 31772323] (IHC-Fr, Mouse)
  
  Details:
  Citation used the Alexa Fluor 700 format of this antibody.
• Lim RR, Hainsworth DP, Mohan RR, Chaurasia SS The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4+ T Cell Peripheralization to Promote Effective Adaptive Immunity Immunity 2019-05-21 [PMID: 31053504] (IHC-Fr)
• An S, Tiruthani K et al. Locally Trapping the C-C Chemokine Receptor Type 7 by Gene Delivery Nanoparticle Inhibits Lymphatic Metastasis Prior to Tumor Resection. Small 2019-01-03 [PMID: 30690891] (IF/IHC, Mouse)
• Klotz L, Norman S, Vieira JM et al. Cardiac lymphatics are heterogeneous in origin and respond to injury. Nature. 2015-05-20 [PMID: 25992544] (Mouse)
• Harrell JC, Pfefferle AD, Zalles N, et al. Endothelial-like properties of claudin-low breast cancer cells promote tumor vascular permeability and metastasis Clin Exp Metastasis 2014-08-22 [PMID: 23975155] (IHC-Fr, Mouse)
• An S, Tiruthani K, Wang Y et al. Locally Trapping the C-C Chemokine Receptor Type 7 by Gene Delivery Nanoparticle Inhibits Lymphatic Metastasis Prior to Tumor Resection Small 2019-01-28 [PMID: 23071319] (IF/IHC, Mouse)
• Mirza M, Pang MF, Zaini MA, et al. Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice PLoS One 2012-05-25 [PMID: 22624044]
• Harada K, Yamazaki T, Iwata C, Yoshimatsu Y, Sase H, Mishima K, Morishita Y, Hirashima M, Oike Y, Suda T, Miura N, Watabe T, Miyazono K. Identification of targets of Prox1 during in vitro vascular differentiation from embryonic stem cells: functional roles of HoxD8 in lymphangiogenesis. J Cell Sci;122(Pt 21):3923-30. 2009-11-01 [PMID: 19825936]
• Luong MX, Tam J, Lin Q, Hagendoorn J, Moore KJ, Padera TP, Seed B, Fukumura D, Kucherlapati R, Jain RK. Lack of lymphatic vessel phenotype in LYVE-1/CD44 double knockout mice. J Cell Physiol;219(2):430-7. 2009-05-01 [PMID: 19170073]
• Jiang S, Bailey AS, Goldman DC, Swain JR, Wong MH, Streeter PR, Fleming WH. Hematopoietic stem cells contribute to lymphatic endothelium. PLoS One;3(11):e3812. 2008-01-01 [PMID: 19043576]
• Semyachkina-Glushkovskaya O, Fedosov I, Shirokov A Et Al. Photomodulation of lymphatic delivery of liposomes to the brain bypassing the blood-brain barrier: new perspectives for glioma therapy Nanophotonics 2021-07-08 (IF/IHC)
• Hirashima M, Sano K, Morisada T, Murakami K, Rossant J, Suda T. Lymphatic vessel assembly is impaired in Aspp1-deficient mouse embryos. Dev Biol;316(1):149-59. 2008-04-01 [PMID: 18304521]
• Mishima K, Watabe T, Saito A, Yoshimatsu Y, Imaizumi N, Masui S, Hirashima M, Morisada T, Oike Y, Araie M, Niwa H, Kubo H, Suda T, Miyazono K. Prox1 induces lymphatic endothelial differentiation via integrin alpha9 and other signaling cascades. Mol Biol Cell;18(4):1421-9. 2007-04-01 [PMID: 17287396]
• Hamaguchi I, Morisada T, Azuma M, Murakami K, Kuramitsu M, Mizukami T, Ohbo K, Yamaguchi K, Oike Y, Dumont DJ, Suda T. Loss of Tie2 receptor compromises embryonic stem cell-derived endothelial but not hematopoietic cell survival. Blood;107(3):1207-13. 2006-02-01 [PMID: 16219799]

Bioinformatics

• Gene Symbol: LYVE1
• Entrez:
  • 114332(Mouse)
• Uniprot:
  • Q8BHC0()