MARCH1 Antibody - BSA Free

Western Blot: MARCH1 Antibody [NBP1-59758] - Human Muscle lysate, concentration 0.2-1 ug/ml.

Western Blot: MARCH1 Antibody [NBP1-59758] - Western blot analysis and gel protein densitometry were used for quantification of MARCH1 (NBP1-59758) and Arginase 1 (NBP1-32731) proteins on transduced macrophage D: The gel image indicates the blot results 48 and 72 hr after transduction, compared with the non-transduced cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35656075/) licensed under a CC-BY license.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

MARCH1 Antibody - BSA Free Summary

• Description: The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
• Immunogen: Synthetic peptides corresponding to MARCH1(membrane-associated ring finger (C3HC4) 1) The peptide sequence was selected from the N terminal of 40238. Peptide sequence LQASSPTTGTAPRSQSRLSVCPSTQDICRICHCEGDEESPLITPCRCTGT. The peptide sequence for this immunogen was taken from within the described region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MARCH1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 ug/ml
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for MARCH1 Antibody - BSA Free

• E3 ubiquitin-protein ligase MARCH1
• EC 6.3.2
• EC 6.3.2.-
• MARCH-IDKFZp564M1682
• membrane-associated ring finger (C3HC4) 1
• Membrane-associated RING finger protein 1
• Membrane-associated RING-CH protein I
• RING finger protein 171
• RNF171FLJ20668

Background

MARCH1 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC, CD86 and FAS, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MARCH1 Antibody (NBP1-59758)(1)

Publication using NBP1-59758

• Zangeneh Z, Khamisipour G, Andalib AR Induced overexpression of MARCH-1 in human macrophages altered to M2 phenotype for suppressing inflammation process Iranian journal of basic medical sciences 2022-04-01 [PMID: 35656075] (WB, Human)

Bioinformatics

• Gene Symbol: MARCH1
• Uniprot:
  • Q8TCQ1-2(Human)