MCT1/SLC16A1 Antibody (882616) [Unconjugated]

Mouse Anti-Human MCT1/SLC16A1 Monoclonal Antibody (Catalog # MAB8275) was detected in (A) HEK293 human embryonic kidney cell line transfected with human MCT1/SLC16A1 and eGFP or (B) Irrelevant transfectants followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 882616
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MCT1/SLC16A1 Antibody (882616) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human MCT1/SLC16A1
  Met1-Val500
  Accession # P53985
• Specificity: Stains human MCT1/SLC16A1 transfected cells but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC16A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCT1/SLC16A1 Antibody (882616) [Unconjugated]

• FLJ36745
• HHF7
• MCT 1
• MCT
• MCT1
• MCT1MGC44475
• member 1
• monocarboxylate transporter 1
• SLC16A1
• Solute carrier family 16 member 1
• solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

Background

Solute Carrier, family 16, member 1 (SLC16A1) encodes Monocarboxylic Acid Transporter 1 (MCT1).  MCT1 is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. MCT1 has been found to be important in lactate uptake in cancer cells, and blockade of MCT1 may inhibit lactate-induced HIF-1 activation (1).

1. Sonveaux, P. et al (2012) PLoS ONE 7:e33418.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCT1/SLC16A1 Antibody (MAB8275)(1)

Publication using MAB8275

• Ruishuang Ma, Xin Li, Shengping Gong, Xiaoqin Ge, Ting Zhu, Xiaoxu Ge et al. Dual Roles of Lactate in EGFR-TKI-Resistant Lung Cancer by Targeting GPR81 and MCT1 Journal of Oncology 2022-12-12 [PMID: 36545125]

Bioinformatics

• Gene Symbol: SLC16A1
• Uniprot:
  • P53985()