Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45).

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (DT101+ BC199 + HMB45).

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: DT101+ BC199 + HMB45
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34608)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant Melanoma Marker (MART-1 + gp100) protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
• Isotype: IgG2b Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLANA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Use in Flow Cytometry reported in scientific literature (PMID: 30735560).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)

• MART1
• MART-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Melanoma Marker (MART-1 + gp100) Antibody (NBP2-34341)(1)

Publication using NBP2-34341

• Po JW, Ma Y, Balakrishna B et al. Immunomagnetic isolation of circulating melanoma cells and detection of PD-L1 status. PLoS One. 2019-02-08 [PMID: 30735560] (FLOW, Human)
  
  Details:
  Citation using the Azide and BSA Free form of this antibody.

Bioinformatics

• Gene Symbol: MLANA
• Uniprot:
  • Q16655()
  • Q16655()
  • Q16655()
  • P40967(Human)