Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Mouse Monoclonal Antibody (DT101+ BC199 + HMB45).

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted [NBP2-48232] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Mouse Monoclonal Antibody (DT101+ BC199 + HMB45).

• Reactivity: Hu
• Applications: IHC
• Clone: DT101+ BC199 + HMB45
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Recombinant Melanoma Marker (MART-1 + gp100) protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
• Isotype: IgG2b Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLANA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) - IHC-Prediluted

• MART1
• MART-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLANA