MMP-16/MT3-MMP Antibody - BSA Free

Western Blot: MMP-16/MT3-MMP Antibody [NBP1-33102] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGEMMP16 antibody dilution: 1:1000

Immunohistochemistry-Paraffin: MMP-16/MT3-MMP Antibody [NBP1-33102] - A549 xenograft , using MMP16 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: MMP-16/MT3-MMP Antibody [NBP1-33102] - (30 ug of whole cell lysate) Hep G2 10% SDS PAGE, antibody diluted at 1:1000.

Western Blot: MMP-16/MT3-MMP Antibody [NBP1-33102] - A. 50 ug mouse kidney lysate/extract 10 % SDS-PAGEMMP16 antibody dilution: 1:1000

• Reactivity: Hu, Mu, Rt, Bv, Ch, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MMP-16/MT3-MMP Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MMP-16/MT3-MMP. The exact sequence is proprietary.
• Localization: Isoform Long: Cell membrane, Single-pass type I membrane protein, Extracellular side (Potential), Isoform Short: Secreted, extracellular space, extracellular matrix, Cell surface
• Predicted Species: Bovine (98%), Xenopus (92%), Chicken (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMP16
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%), Japanese Medaka (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MMP-16/MT3-MMP Antibody - BSA Free

• chromosome 8 open reading frame 57
• DKFZp761D112
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.80
• matrix metallopeptidase 16 (membrane-inserted)
• matrix metalloproteinase 16 (membrane-inserted)
• matrix metalloproteinase-16
• Membrane-type matrix metalloproteinase 3
• Membrane-type-3 matrix metalloproteinase
• MMP16
• MMP-16
• MMPX2
• MMP-X2
• MT3MMP
• MT3-MMP
• MT3-MMPC8orf57
• MT-MMP 3
• MT-MMP2
• MTMMP3
• MT-MMP3
• Putative transmembrane protein C8orf57

Background

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen types I, II and III. MMP-13 has wide substrate specificity, and its physiologic expression is limited to situations in which rapid and effective remodeling of collagenous ECM takes place, such as fetal bone development and adult bone remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MMP16