MMP-3 Antibody - BSA Free

Western Blot: MMP-3 Antibody [NB100-91878] - Analysis of MMP-3 (S453) antibody in extracts from 293 cells.

Immunohistochemistry-Paraffin: MMP-3 Antibody [NB100-91878] - Analysis of mouse brain.

Immunohistochemistry-Paraffin: MMP-3 Antibody [NB100-91878] - Analyzes of paraffin-embedded human breast carcinoma tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MMP-3 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an C-terminal portion of the human MMP3 protein (between residues 400-477) [UniProt P08254]
• Marker: Mesenchymal Cells Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500- 1:1000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MMP-3 Antibody - BSA Free

• CHDS6
• EC 3.4.24
• EC 3.4.24.17
• matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• matrix metalloproteinase 3 (stromelysin 1, progelatinase)
• Matrix metalloproteinase-3
• MGC126102
• MGC126103
• MMP3
• MMP-3
• proteoglycanase
• SL-1
• STMY
• STMY1MGC126104
• STR1
• Stromelysin 1
• stromelysin-1
• transin-1

Background

Matrix metalloproteinase 3 (MMP3), also known as Stromelysin-1, SL-1, and STMY1, is a member of the matrix metalloproteinase (MMP) family. Members of the MMP family are involved in the breakdown of the extracellular matrix in response to normal physiological and disease processes; these processes include embryonic development, reproduction, tissue remodeling, arthritis and metastasis. The majority of MMP proteins are secreted into the extracellular matrix as inactive proproteins; they are activated when cleaved by an extracellular proteinase. The MMP3 gene contains four hemopexin-like domains, and is part of a cluster of MMP genes that localize to chromosome 11q22.3. This gene encodes the MMP3 enzyme that degrades fibronectin, laminin, gelatins (type I, III, IV and V), collagens (type III, IV, X, and IX), and cartilage proteoglycans. Additionally, the MMP3 enzyme activates procollagenase. Areas of interest for MMP3 include wound repair, progression of atherosclerosis, and tumor initiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-3 Antibody (NB100-91878)(9)

Publications using NB100-91878

• Wang J, Huang L, Yang X Et al. The Regulatory Effect of MicroRNA-101-3p on Disc Degeneration by the STC1/VEGF/MAPK Pathway Oxidative medicine and cellular longevity 2021-10-05 [PMID: 34650661] (WB, Human)
• Ma X, Zhang Z, Shen M et al. Changes of type II collagenase biomarkers on IL-1beta-induced rat articular chondrocytes Experimental and therapeutic medicine 2021-06-01 [PMID: 33850554] (WB, Rat)
• Boukhedouni N, Martins C, Darrigade AS et al. Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo JCI Insight 2020-06-04 [PMID: 32369451] (IHC-P, Human)
• Lin D, Alberton P, Delgado Caceres M et al. Loss of tenomodulin expression is a risk factor for age-related intervertebral disc degeneration Aging Cell 2020-02-21 [PMID: 32083813] (IF/IHC, Mouse)
• Zhuo W, Li B, Zhang D et al. Activation of G-protein-coupled bile acid receptor Gpbar1 (TGR5) inhibits degradation of type II collagen and aggrecan in human chondrocytes Eur. J. Pharmacol. 2019-05-07 [PMID: 31075239] (Human)
• Mirastschijski U, Dinesh N, Baskaran S et al. Novel specific human and mouse stromelysin-1 (MMP-3) and stromelysin-2 (MMP-10) antibodies for biochemical and immunohistochemical analyses Wound Repair Regen 2019-02-14 [PMID: 30761682] (WB, Human)
• De Cunto G, Lamberti A, de Santi MM et al. Middermal Elastolysis: Dermal Fibroblasts Cooperate with Inflammatory Cells to the Elastolytic Disorder Mediators Inflamm. 2017-09-16 [PMID: 29097850] (IF/IHC, Human)
• Huang W, Ao P, Li J et al. Autophagy protects advanced glycation end product-induced apoptosis and. BioMed Research International. 2017-01-16 [PMID: 28265573] (WB, Rat)
• Wu Y Lee MJ Ido Y, Fried SK. High fat diet-induced obesity regulates MMP3 to modulate depot- and sex-dependent adipose expansion in C57BL/6J mice. Am. J. Physiol. Endocrinol. Metab. 2016-11-22 [PMID: 27879248] (WB, Mouse)

Review for MMP-3 Antibody (NB100-91878) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human and Mouse.

reviewed by: Verified Customer WB Human and Mouse 04/25/2024

Summary

• Application: Western Blot
• Sample Tested: human lung cancer,Human lung macrophages
• Species: Human and Mouse
• Lot: A-4

Bioinformatics

• Gene Symbol: MMP3
• Entrez:
  • 4314(Human)
  • 17392(Mouse)
  • 171045(Rat)
• Uniprot:
  • P08254()
  • P28862()