Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IFNL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA

• IFNl2
• IFNlambda 2
• IL28A
• IL-28A
• IL-28A/B (IFN-lambda 2/3)
• IL28B
• interferon lambda 2

Background

Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.

Publications for IL-28A/B (IFN-lambda 2/3) (DY1789B)(24)

Publications using DY1789B

• Sharma, L;Singh, RB;Ngeow, C;van der Geest, R;Duray, AM;Tolman, NJ;McVerry, BJ;Dela Cruz, CS;Alcorn, JF;Bain, W;Robinson, KM; Therapeutic JAK inhibition does not impact lung injury during viral or bacterial pneumonia in male mice Physiological reports 2025-02-01 [PMID: 39921246] (Mouse)
• Gaur, KK;Asuru, TR;Srivastava, M;Singh, N;Purushotham, N;Poojary, B;Das, B;Bhattacharyya, S;Asthana, S;Guchhait, P; 7D, a small molecule inhibits dengue infection by increasing interferons and neutralizing-antibodies via CXCL4:CXCR3:p38:IRF3 and Sirt1:STAT3 axes respectively EMBO molecular medicine 2024-09-16 [PMID: 39284947] (Mouse)
• De, M;Sukla, S;Bharatiya, S;Keshri, S;Roy, DG;Roy, S;Dutta, D;Saha, S;Ejazi, SA;Ravichandiran, V;Ali, N;Chatterjee, M;Chinnaswamy, S; IFN-?3 is induced by Leishmania donovani and can inhibit parasite growth in cell line models but not in the mouse model, while it shows a significant association with leishmaniasis in humans Infection and immunity 2024-01-09 [PMID: 38193711] (Mouse)
• Hilligan, KL;Namasivayam, S;Clancy, CS;Baker, PJ;Old, SI;Peluf, V;Amaral, EP;Oland, SD;O'Mard, D;Laux, J;Cohen, M;Garza, NL;Lafont, BAP;Johnson, RF;Feng, CG;Jankovic, D;Lamiable, O;Mayer-Barber, KD;Sher, A; Bacterial-induced or passively administered interferon gamma conditions the lung for early control of SARS-CoV-2 Nature communications 2023-12-12 [PMID: 38086794] (Mouse)
• Pan, L;Xue, Y;Wang, K;Zheng, X;Islam, A;Tapryal, N;Chakraborty, A;Bacsi, A;Ba, X;Hazra, TK;Boldogh, I; Nei-like DNA glycosylase 2 (NEIL2) selectively antagonizes interferon-beta expression upon respiratory syncytial virus infection The Journal of biological chemistry 2023-07-07 [PMID: 37423306] (Mice)
• Alexis R. Gibson, Adam Sateriale, Jennifer E. Dumaine, Julie B. Engiles, Ryan D. Pardy, Jodi A. Gullicksrud et al. A genetic screen identifies a protective type III interferon response to Cryptosporidium that requires TLR3 dependent recognition PLOS Pathogens 2022-05-18 [PMID: 35584177] (Mouse)
• M Le Roux, A Ollivier, G Kervoaze, T Beke, L Gillet, M Pichavant, P Gosset IL-20 Cytokines Are Involved in Epithelial Lesions Associated with Virus-Induced COPD Exacerbation in Mice Biomedicines, 2021-12-05;9(12):. 2021-12-05 [PMID: 34944654] (Mouse)
• HE Rich, D Antos, CC McCourt, WQ Zheng, LJ Devito, KJ McHugh, R Gopal, J Wang, JF Alcorn Murine Type III interferons are functionally redundant and correlate with bacterial burden during influenza/bacterial super-infection PLoS ONE, 2021-10-07;16(10):e0255309. 2021-10-07 [PMID: 34618816] (Mouse)
• S Lee, G Kalugotla, H Ingle, R Rodgers, C Wu, Y Wang, Y Li, X Yang, J Zhang, NR Borella, H Deng, L Droit, R Hill, ST Peterson, C Desai, D Lawrence, Q Lu, MT Baldridge Intestinal antiviral signaling is controlled by autophagy gene Epg5 independent of the microbiota Autophagy, 2021-09-14;0(0):1-16. 2021-09-14 [PMID: 34520306] (Mouse)
• YA Ko, YH Yu, YF Wu, YC Tseng, CL Chen, KS Goh, HY Liao, TH Chen, TR Cheng, AS Yang, CH Wong, C Ma, KI Lin A non-neutralizing antibody broadly protects against influenza virus infection by engaging effector cells PloS Pathogens, 2021-08-05;17(8):e1009724. 2021-08-05 [PMID: 34352041] (Mouse)
• J Xing, X Zhou, M Fang, E Zhang, LJ Minze, Z Zhang DHX15 is required to control RNA virus-induced intestinal inflammation Cell Reports, 2021-06-22;35(12):109205. 2021-06-22 [PMID: 34161762] (Mouse)
• H Ingle, E Hassan, J Gawron, B Mihi, Y Li, EA Kennedy, G Kalugotla, H Makimaa, S Lee, P Desai, KG McDonald, MS Diamond, RD Newberry, M Good, MT Baldridge Murine astrovirus tropism for goblet cells and enterocytes facilitates an IFN-&lambda response in vivo and in enteroid cultures Mucosal Immunology, 2021-03-05;0(0):. 2021-03-05 [PMID: 33674763] (Mouse)
• RR Goel, X Wang, LJ O'Neil, S Nakabo, K Hasneen, S Gupta, G Wigerblad, LP Blanco, JB Kopp, MI Morasso, SV Kotenko, ZX Yu, C Carmona-Ri, MJ Kaplan Interferon lambda promotes immune dysregulation and tissue inflammation in TLR7-induced lupus Proc. Natl. Acad. Sci. U.S.A., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094169] (Mouse)
• JM Veazey, TJ Chapman, TR Smyth, SE Hillman, SI Eliseeva, SN Georas Distinct roles for MDA5 and TLR3 in the acute response to inhaled double-stranded RNA PLoS ONE, 2019-05-08;14(5):e0216056. 2019-05-08 [PMID: 31067281] (Mouse)
• A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations Nat Commun, 2018-06-08;9(1):2229. 2018-06-08 [PMID: 29884817] (Mouse)
• Sujin An, Yung Jin Jeon, Ara Jo, Hyun Jung Lim, Young Eun Han, Sung Woo Cho et al. Initial Influenza Virus Replication Can Be Limited in Allergic Asthma Through Rapid Induction of Type III Interferons in Respiratory Epithelium Frontiers in Immunology 2018-05-17 [PMID: 29867963] (Mouse)
• IE Galani, V Triantafyl, EE Eleminiado, O Koltsida, A Stavropoul, M Manioudaki, D Thanos, SE Doyle, SV Kotenko, K Thanopoulo, E Andreakos Interferon-? Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness Immunity, 2017-05-16;46(5):875-890.e6. 2017-05-16 [PMID: 28514692] (Mouse)
• Joerg Mattes TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection Am. J. Physiol. Lung Cell Mol. Physiol., 2016-11-11;0(0):ajplung.00200. 2016-11-11 [PMID: 27836899] (Mouse)
• von Buttlar H, Siegemund S, Buttner M, Alber G Identification of Toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells. PLoS ONE, 2014-08-29;9(8):e106188. 2014-08-29 [PMID: 25171368] (Mouse)
• Spann K, Loh Z, Lynch J, Ullah A, Zhang V, Baturcam E, Werder R, Khajornjiraphan N, Rudd P, Loo Y, Suhrbier A, Gale M, Upham J, Phipps S IRF-3, IRF-7, and IPS-1 promote host defense against acute human metapneumovirus infection in neonatal mice. Am J Pathol, 2014-04-13;184(6):1795-806. 2014-04-13 [PMID: 24726644] (Mouse)
• Okamoto , Masaaki, Oshiumi , Hiroyuki, Azuma , Masahiro, Kato , Nobuyuki, Matsumoto , Misako, Seya , Tsukasa IPS-1 is essential for type III IFN production by hepatocytes and dendritic cells in response to hepatitis C virus infection. J Immunol, 2014-02-14;192(6):2770-7. 2014-02-14 [PMID: 24532585] (Mouse)
• Jayaraman A, Jackson D, Message S, Pearson R, Aniscenko J, Caramori G, Mallia P, Papi A, Shamji B, Edwards M, Westwick J, Hansel T, Stanciu L, Johnston S, Bartlett N IL-15 complexes induce NK- and T-cell responses independent of type I IFN signaling during rhinovirus infection. Mucosal Immunol, 2014-01-29;7(5):1151-64. 2014-01-29 [PMID: 24472849] (Mouse)
• Crotta S, Davidson S, Mahlakoiv T, Desmet C, Buckwalter M, Albert M, Staeheli P, Wack A Type I and type III interferons drive redundant amplification loops to induce a transcriptional signature in influenza-infected airway epithelia. PLoS Pathog, 2013-11-21;9(11):e1003773. 2013-11-21 [PMID: 24278020] (Mouse)
• Steinberg C, Eisenacher K, Gross O, Reindl W, Schmitz F, Ruland J, Krug A The IFN regulatory factor 7-dependent type I IFN response is not essential for early resistance against murine cytomegalovirus infection. Eur. J. Immunol., 2009-04-01;39(4):1007-18. 2009-04-01 [PMID: 19283778] (Mouse)

FAQs for IL-28A/B (IFN-lambda 2/3) (DY1789B). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IFNL3