MTHFD2 Antibody

Western Blot: MTHFD2 Antibody [NBP1-33200] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MTHFD2 Antibody [NBP1-33200] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTHFD2 stained by MTHFD2 antibody [N1C3] diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded human MCF7 xenograft. MTHFD2 antibody [N1C3] diluted at 1:500.

Western Blot: MTHFD2 Antibody [NBP1-33200] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded human breast carcinoma diluted at 1:500.

Immunoprecipitation: MTHFD2 Antibody [NBP1-33200] - Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 ug of MTHFD2 antibody [N1C3] Western blot analysis was performed using MTHFD2 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: MTHFD2 Antibody [NBP1-33200] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] (NBP1-33200) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.MTHFD2 stained by MTHFD2 antibody [N1C3] (NBP1-33200) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: MTHFD2 Antibody [NBP1-33200] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MTHFD2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Mouse (95%), Rat (93%), Chicken (92%), Xenopus (90%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTHFD2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (86%), Xenopus laevis (90%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for MTHFD2 Antibody

• bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase
• methenyltetrahydrofolate cyclohydrolase
• methylene tetrahydrofolate dehydrogenase (NAD+ dependent)
• methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2
• mitochondrial
• NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase
• NMDMC

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MTHFD2 Antibody (NBP1-33200)(2)

Publications using NBP1-33200

• Wang W, Gu W, Tang H et al. The Emerging Role of MTHFD Family Genes in Regulating the Tumor Immunity of Oral Squamous Cell Carcinoma Journal of oncology 2022-06-03 [PMID: 35693982] (IF/IHC)
• Moran DM, Trusk PB, Pry KJ et al. KRAS mutation status is associated with enhanced dependency on folate metabolism pathways in Non Small Cell Lung Cancer cells. Mol. Cancer Ther. 2014-03-31 [PMID: 24688052] (IHC-P, Human)

Bioinformatics

• Gene Symbol: MTHFD2