MUC2 Antibody - BSA Free

Immunohistochemistry: MUC2 Antibody [NBP1-31231] - MUC2 and Cadherin-17 were detected in immersion fixed, paraffin-embedded sections of MimEX adult stem cell-derived ascending colon tissue with Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) at 1.25 ug/mL and Rabbit Anti-Human/Mouse (Catalog # NBP1-31231) at 0.62 ug/mL overnight at 4 C. Tissue was then stained using the NorthernLights 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Cadherin-17 staining was detected in cellular membranes throughout the epithelial layer while MUC2 staining was localized specifically to goblet cells.

Immunohistochemistry: MUC2 Antibody [NBP1-31231] - Effect of SPI on mucin production in mouse ileum tissue. Representative micrographs for MUC2 staining and corresponding quantification of MUC2+ goblet cells per villus in the ileum tissue. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32911830) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: MUC2 Antibody [NBP1-31231] - Milk-derived exosomes promote goblet cell expression in vitro. Representative micrographs for MUC2 immunofluorescent staining in LS174T control and exosome-treated cells (white arrows show positive stain). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30699187/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] - Mouse intestine.MUC2 stained by MUC2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -Mouse colon.MUC2 stained by MUC2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -Mouse colon.MUC2 stained by MUC2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] - Mouse intestine.MUC2 stained by MUC2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -Human intestine.MUC2 antibody [C3], C-term diluted at 1:750.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -Rat intestine.MUC2 stained by MUC2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, DB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MUC2 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MUC2. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MUC2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dot Blot Reported in scientific literature (PMID:32223302).
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 540 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for MUC2 Antibody - BSA Free

• Intestinal mucin-2
• MLP
• MUC2
• MUC-2
• mucin 2, intestinal/tracheal
• mucin 2, oligomeric mucus/gel-forming
• mucin-2
• SMUC

Background

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC2 Antibody (NBP1-31231)(46)

Publications using NBP1-31231

• Quon T, Lin LC, Ganguly A et Al. Biased constitutive signaling of the G protein-coupled receptor GPR35 suppresses gut barrier permeability J Biol Chem 2024-11-29 [PMID: 39615676]
• G�mez-Belmonte R, Wagner Y, Mahapatro M et al. Intestinal epithelial Gasdermin C is induced by IL-4R/STAT6 signaling but is dispensable for gut immune homeostasis Scientific Reports 2024-11-03 [PMID: 39489845]
• Sato K, Hara-Chikuma M, Yasui M, Inoue J et Al. Sufficient water intake maintains the gut microbiota and immune homeostasis and promotes pathogen elimination iScience 2024-05-27 [PMID: 38799550]
• Eshleman EM, Rice T, Potter C, Waddell A et Al. Microbiota-derived butyrate restricts tuft cell differentiation via histone deacetylase 3 to modulate intestinal type 2 immunity Immunity 2024-01-31 [PMID: 38295798]
• Cheng L, Wu H, Cai X, Zhang Y et Al. A Gpr35-tuned gut microbe-brain metabolic axis regulates depressive-like behavior Cell Host Microbe 2024-01-10 [PMID: 38198925]
• HAfliger J, Schwarzfischer M, Atrott K et al. Glycoprotein (GP)96 Is Essential for Maintaining Intestinal Epithelial Architecture by Supporting Its Self-Renewal Capacity Cellular and molecular gastroenterology and hepatology 2022-12-11 [PMID: 36516930] (IHC-P, Mouse)
• Luck H The Role of the Intestinal Immune System in Diet-induced Obesity and Insulin Resistance Thesis 2020-01-01 (ICC/IF, Mouse)
• Judith C. W. de Jong, Kristel S. van Rooijen, Edwin C. A. Stigter, M. Can Gülersönmez, Marcel R. de Zoete, Janetta Top, Matthijs J. D. Baars, Yvonne Vercoulen, Folkert Kuipers, Saskia W. C. van Mil, Noortje Ijssennagger, Birendra Mishra Dietary cystine restriction increases the proliferative capacity of the small intestine of mice PLOS ONE 2024-01-05 [PMID: 38181033]
• Chamignon C, Mallaret G, Rivi�re J et al. Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex Biomolecules 2023-08-24 [PMID: 37759696] (Human, Immunohistochemistry-Paraffin)
• Efren A. Reyes, David Castillo-Azofeifa, Jérémie Rispal, Tomas Wald, Rachel K. Zwick, Brisa Palikuqi, Angela Mujukian, Shervin Rabizadeh, Alexander R. Gupta, James M. Gardner, Dario Boffelli, Zev J. Gartner, Ophir D. Klein Epithelial TNF controls cell differentiation and CFTR activity to maintain intestinal mucin homeostasis The Journal of Clinical Investigation 2023-10-16 [PMID: 37643009]
• Sonomoto K, Song R, Eriksson D et al. High-fat-diet-associated intestinal microbiota exacerbates psoriasis-like inflammation by enhancing systemic ?? T�cell IL-17 production Cell Reports 2023-07-25 [PMID: 37421628] (Human, Immunohistochemistry-Paraffin)
• Chae SA, Du M, Son JS, Zhu MJ Exercise improves homeostasis of the intestinal epithelium by activation of apelin receptor-AMP-activated protein kinase signalling The Journal of physiology 2023-05-08 [PMID: 37154385]
• Oosterlinck B, Ceuleers H, Arras W et al. Mucin-microbiome signatures shape the tumor microenvironment in gastric cancer Microbiome 2023-04-21 [PMID: 37085819] (Human, Immunohistochemistry-Paraffin)
• Castillo-Azofeifa D, Wald T, Reyes EA et al. A DLG1-ARHGAP31-CDC42 axis is essential for the intestinal stem cell response to fluctuating niche Wnt signaling Cell stem cell 2023-01-05 [PMID: 36640764] (IHC-Fr, IHC-P, Mouse)
• Breugelmans T, Arras W, Boen LE et al. Aberrant Mucin Expression Profiles Associate With Pediatric Inflammatory Bowel Disease Presentation and Activity Inflammatory bowel diseases 2022-10-14 [PMID: 36239641]
• Chae SA, Son JS, de Avila JM et al. Maternal exercise improves epithelial development of fetal intestine by enhancing apelin signaling and oxidative metabolism American journal of physiology. Regulatory, integrative and comparative physiology 2022-11-01 [PMID: 36189989] (IHC-P, WB, Mouse)
  
  Details:
  Dilution used for IHC 1:50, for WB 1:1000
• Palikuqi B, Rispal J, Reyes EA et al. Lymphangiocrine signals are required for proper intestinal repair after cytotoxic injury Cell stem cell 2022-08-04 [PMID: 35931034] (IHC-Fr, Mouse)
• Du J, Sarkar R, Li Y et al. N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Developmental cell 2022-08-22 [PMID: 35917813] (Human, Immunohistochemistry-Paraffin)
• Lacroix G, Gouyer V, Rocher M et al. A porous cervical mucus plug leads to preterm birth induced by experimental vaginal infection in mice iScience 2022-07-15 [PMID: 35754724] (Mouse)
• Das TK, Blasco-Conesa MP, Korf J et al. Bacterial Amyloid Curli Associated Gut Epithelial Neuroendocrine Activation Predominantly Observed in Alzheimer's Disease Mice with Central Amyloid-? Pathology Journal of Alzheimer's Disease 2022-08-30 [PMID: 35527554] (Human, Immunohistochemistry-Paraffin)
• Zangara, M;Ponti, A;Miller, N;Engelhart, M;Ahern, P;Sangwan, N;McDonald, C; Maltodextrin Consumption Impairs the Intestinal Mucus Barrier and Accelerates Colitis Through Direct Actions on the Epithelium Frontiers in Immunology [PMID: 35359925] (ICC/IF, Mouse)
• Higa, T;Okita, Y;Matsumoto, A;Nakayama, S;Oka, T;Sugahara, O;Koga, D;Takeishi, S;Nakatsumi, H;Hosen, N;Robine, S;Taketo, MM;Sato, T;Nakayama, KI; Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium Nature communications [PMID: 35314700] (ICC/IF, Mouse)
• Melhem H, Kaya B, Kaymak T et al. Epithelial GPR35 protects from Citrobacter rodentium infection by preserving goblet cells and mucosal barrier integrity Mucosal Immunology 2022-03-09 [PMID: 35264769] (Human, Immunohistochemistry-Paraffin)
• Daniel Zeve, Eric Stas, Joshua de Sousa Casal, Prabhath Mannam, Wanshu Qi, Xiaolei Yin, Sarah Dubois, Manasvi S. Shah, Erin P. Syverson, Sophie Hafner, Jeffrey M. Karp, Diana L. Carlone, Jose Ordovas-Montanes, David T. Breault Robust differentiation of human enteroendocrine cells from intestinal stem cells Nature Communications 2022-01-11 [PMID: 35017529]
• Noortje Ijssennagger, Kristel S. van Rooijen, Stefanía Magnúsdóttir, José M. Ramos Pittol, Ellen C.L. Willemsen, Marcel R. de Zoete, Matthijs J.D. Baars, Paul B. Stege, Carolina Colliva, Roberto Pellicciari, Sameh A. Youssef, Alain de Bruin, Yvonne Vercoulen, Folkert Kuipers, Saskia W.C. van Mil Ablation of liver Fxr results in an increased colonic mucus barrier in mice JHEP Reports 2021-08-04 [PMID: 34604725]
• Kwon MS, Chung HK, Xiao L et al. MicroRNA-195 regulates Tuft cell function in the intestinal epithelium by altering translation of double cortin-like kinase 1 American journal of physiology. Cell physiology 2021-03-31 [PMID: 33788631]
• Tomita H, Tanaka K, Hirata A et al. Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas Cell reports 2021-02-23 [PMID: 33626352]
• Tirado FR, Bhanja P, Castro-Nallar E, et al. Radiation-induced toxicity in rectal epithelial stem cell contributes to acute radiation injury in rectum Stem cell research & therapy 2021-01-15 [PMID: 33451351] (ICC/IF, Mouse)
• Rishi Drolia, Mary Anne Roshni Amalaradjou, Valerie Ryan, Shivendra Tenguria, Dongqi Liu, Xingjian Bai, Luping Xu, Atul K. Singh, Abigail D. Cox, Victor Bernal-Crespo, James A. Schaber, Bruce M. Applegate, Ramesh Vemulapalli, Arun K. Bhunia Receptor-targeted engineered probiotics mitigate lethal Listeria infection Nature Communications 2020-12-11 [PMID: 33311493]
• Engevik M, Danhof HA, Auchtung J et al. Fusobacterium nucleatum adheres to Clostridioides difficile via the RadD adhesin to enhance biofilm formation in intestinal mucus Gastroenterology 2020-11-20 [PMID: 33227279]
• Ludikhuize MC, Meerlo M, Gallego MP et al. Mitochondria Define Intestinal Stem Cell Differentiation Downstream of a FOXO/Notch Axis BMC Neurosci 2020-10-31 [PMID: 33147486]
• Shastri S, Shinde T, Perera AP et al. Idebenone Protects against Spontaneous Chronic Murine Colitis by Alleviating Endoplasmic Reticulum Stress and Inflammatory Response Biomedicines 2020-09-28 [PMID: 32998266]
• Zeng B, Wang D, et al. Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein. Nutrients 2020-09-08 [PMID: 32911830] (IF/IHC, Mouse)
• Yan J, Wang D, Li K et al. Toxic effects of the food additives titanium dioxide and silica on the murine intestinal tract: Mechanisms related to intestinal barrier dysfunction involved by gut microbiota Environmental toxicology and pharmacology 2020-09-03 [PMID: 32891757] (IHC-P, Mouse)
• Engevik MA, Danhof HA, Shrestha R et al. Reuterin disrupts Clostridioides difficile metabolism and pathogenicity through reactive oxygen species generation Gut Microbes 2020-11-09 [PMID: 32804011] (IF/IHC, Human)
• Engevik MA, Danhof HA, Chang-Graham AL et al. Human intestinal enteroids as a model of Clostridioides difficile-induced enteritis Am. J. Physiol. Gastrointest. Liver Physiol. 2020-05-01 [PMID: 32223302] (Cytometric Bead Assay Standard)
• Schwerdtfeger LA, Tobet SA Vasoactive intestinal peptide regulates ileal goblet cell production in mice Physiol Rep 2020-02-01 [PMID: 32026594] (IF/IHC, Mouse)
• Breugelmans T, Van Spaendonk H, De Man JG et al. In depth study of transmembrane mucins in association with intestinal barrier dysfunction during the course of T cell transfer and DSS-induced colitis J Crohns Colitis 2020-01-31 [PMID: 32003421]
• Jarret A, Jackson R, Duizer C et al. Enteric Nervous System-Derived IL-18 Orchestrates Mucosal Barrier Immunity Cell 2020-01-09 [PMID: 31923399]
• Seo B, Jeon K, Moon S, et al. Roseburia spp. Abundance Associates with Alcohol Consumption in Humans and Its Administration Ameliorates Alcoholic Fatty Liver in Mice Cell Host Microbe 2019-12-19 [PMID: 31866426] (Mouse)
• Miyake H, Lee C, Chusilp S et al. Human breast milk exosomes attenuate intestinal damage Pediatr. Surg. Int. 2019-11-12 [PMID: 31713717]
• Li B, Hock A, Wu RY et al. Bovine milk-derived exosomes enhance goblet cell activity and prevent the development of experimental necrotizing enterocolitis PLoS ONE 2019-01-30 [PMID: 30699187] (ICC/IF, Human)
• de Jong J, van Rooijen K, Stigter E et al. Dietary cystine restriction increases the proliferative capacity of the small intestine of mice bioRxiv 2023-08-12 (IHC, Mouse)
• Janardhan KS, Jensen H, Clayton NP, Herbert RA Immunohistochemistry in Investigative and Toxicologic Pathology Toxicol Pathol. 2018-07-01 [PMID: 29966501] (IF/IHC, IF/IHC, Mouse)
  
  Details:
  Citation used the HRP format of this antibody.
• Yasuda-Onozawa Y, Handa O et al. Rebamipide upregulates mucin secretion of intestinal goblet cells via Akt phosphorylation. Mol Med Rep 2017-01-12 [PMID: 28983630] (Dot Blot, Human)
• Kameoka Y, Kitazawa R, Ariasu K et al. Reactivation of CDX2 in Gastric Cancer as Mark for Gene Silencing Memory. Acta Histochem Cytochem 2015-08-27 [PMID: 26379313] (IHC-P, Human)

Bioinformatics

• Gene Symbol: MUC2