Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.

Immunoprecipitation: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32169] - Whole cell lysate 293T cells.

• Reactivity: Hu, Mu, Ca, Eq
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Phosphofructokinase, Muscle using the numbering given in entry NP_000280.1 (GeneID 5213).
• Predicted Species: Canine (100%), Equine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PFKM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Simple Western
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody - BSA Free

• 6-phosphofructokinase, muscle type
• EC 2.7.1,6-phosphofructo-1-kinase
• EC 2.7.1.11
• GSD7
• MGC8699
• Muscle Phosphofructokinase
• PFK1
• PFK-1
• PFKA
• PFK-A
• PFKM
• PFKX
• Phosphofructo-1-kinase isozyme A
• Phosphofructokinase 1
• phosphofructokinase, muscle
• phosphofructokinase, polypeptide X
• Phosphofructokinase-1
• Phosphofructokinase-M
• Phosphohexokinase

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (NBP2-32169)(1)

Publication using NBP2-32169

• Urasaki, Y, Fiscus, R R Et al. Molecular classification of fatty liver by high-throughput profiling of protein post-translational modifications. J Pathol 2016-04-01 [PMID: 26725750] (IF/IHC, Mouse)

Review for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (NBP2-32169) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 11/12/2015

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: PFKM
• Uniprot:
  • NP_000280.1(Human)