MxA/Mx1 Antibody - BSA Free

Western Blot: MxA/Mx1 Antibody [NBP1-32905] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MxA/Mx1 Antibody [NBP1-32905] - Detects MX1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MX1 protein stained by MX1 antibody, Internal diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905] - Human lymph node. Antigen retrieval in citrate buffer, 95C for 30 min. Image submitted by a verified customer review.

Western Blot: MxA/Mx1 Antibody [NBP1-32905] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human MX1-transfected 293T cells. 7.5 % SDS-PAGE MX1 antibody [N2C2], Internal dilution: 1:10000

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905] - MDA-MB-468 xenograft, using MX1antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody [NBP1-32905] - Human colon carcinoma. MX1 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Bv, Pm, Rb, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MxA/Mx1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MxA/Mx1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Chimpanzee (98%), Rhesus Macaque (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for MxA/Mx1 Antibody - BSA Free

• human interferon-regulated resistance GTP-binding protein MXA10IFI-78Khomolog of murine (interferon-inducibleprotein p78)
• IFI78
• IFI-78K
• Mx1
• MxA
• myxovirus (influenza virus) resistance 1, interferon-inducible protein p78(mouse)

Background

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MxA/Mx1 Antibody (NBP1-32905)(1)

Publication using NBP1-32905

• Alalem M, Dabous E, Awad AM et al. Influenza a virus regulates interferon signaling and its associated genes; MxA and STAT3 by cellular miR-141 to ensure viral replication Virology Journal 2023-08-18 [PMID: 37596622]

Review for MxA/Mx1 Antibody (NBP1-32905) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Santhosh Sivajothi IHC-P Human 09/25/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human lymph node
• Species: Human
• Lot: 40037

Bioinformatics

• Gene Symbol: MX1