Human Myoglobin ELISA Kit (Chemiluminescence)

ELISA: Human Myoglobin ELISA Kit (Chemiluminescence) [NBP2-68064] - Samples were spiked with high concentrations of Human Myoglobin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Myoglobin ELISA Kit (Chemiluminescence) [NBP2-68064] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.63-40 ng/mL
• Sensitivity: 0.38 ng/mL

Human Myoglobin ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human MYO in samples. No significant cross-reactivity or interference between Human MYO and analogues was observed.
• Standard Curve Range: 0.63-40 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 11.08%
• Intra-Assay: CV% < 10.26%
• Spike Recovery: 88-113%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: MB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Myoglobin ELISA Kit (Chemiluminescence)

• MB
• MGC13548
• Myoglobin
• PVALB

Background

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MB