Recombinant Human Myoglobin Protein

• Reactivity: Hu

Recombinant Human Myoglobin Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to Myoglobin Source: E. coli

  Amino Acid Sequence: GLSDGEWQLV LNVWGKVEAD IPGHGQEVLI RLFKGHPETL EKFDKFKHLK SEDEMKASED LKKHGATVLT ALGGILKKKG HHEAEIKPLA QSHATKHKIP VKYLEFISEC IIQVLQSKHP GDFGADAQGA MNKALELFRK DMASNYKELG FQG

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MB
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Less than 1.0 EU/ug of Myoglobin as determined by LAL method.

Applications/Dilutions

• Theoretical MW: 17.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: 0.2 um filtered solution in 25 mM Tris-HCl (pH 8.0)
• Preservative: 50% glycerol
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Myoglobin Protein

• MB
• MGC13548
• Myoglobin
• PVALB

Background

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MB