N-Cadherin Antibody [Unconjugated] - Propeptide

Western blot shows lysates of IMR-32 human neuroblastoma cell line and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human N-Cadherin Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1388) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Pro-N-Cadherin was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

N-Cadherin was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1388) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks between the myocardial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

N-Cadherin Antibody [Unconjugated] - Propeptide Summary

• Immunogen: E. coli-derived recombinant human N-Cadherin Propeptide
  Ser26-Arg159
  Accession # P19022
• Specificity: Detects human N-Cadherin Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Cadherin-8, rhCadherin-11, and rhR-Cadherin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CDH2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for N-Cadherin Antibody [Unconjugated] - Propeptide

• ACOGS
• ARVD14
• cadherin 2, type 1, N-cadherin (neuronal)
• Cadherin-2
• calcium-dependent adhesion protein, neuronal
• CD325 antigen
• CD325
• CDH2
• CDHN
• CDw325
• NCAD
• N-cadherin 1
• NCadherin
• N-Cadherin
• Neural cadherin
• neural-cadherin

Background

N‑Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130‑135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N‑Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN‑Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15‑20 kDa prodomain (aa 26‑159) is cleaved by proprotein convertase, and the mature molecule is transported to the surface. Alternatively, on neurons, proN‑Cadherin may first appear on the surface, with cleavage occurring at the time of synaptogenesis. Cleavage appears necessary for homophilic interaction as presence of the prodomain is suggested to negatively regulate oligomer formation. Over the entire prodomain, the human N‑Cadherin proregion shares 87% aa identity with the mouse N‑Cadherin proregion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N-Cadherin Antibody (AF1388)(1)

Publication using AF1388

• Harrison OJ, Corps EM, Kilshaw PJ Cadherin adhesion depends on a salt bridge at the N-terminus. J. Cell. Sci., 2005-08-23;118(0):4123-30. 2005-08-23 [PMID: 16118243] (Flow Cytometry, Chicken)

Bioinformatics

• Gene Symbol: CDH2
• Uniprot:
  • P19022()