Nanog Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - Nanog-inducible mouse model. Immunohistochemistry (IHC) for NANOG of paraffin-embedded sections of tail skin from CTR and TG mice treated as indicated in (B). Two magnifications are shown for each tissue (bars correspond to 50 um). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license.

Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog staining in ES cells treatment with leukemia inhibitory factor (LIF). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.

Immunohistochemistry: Nanog Antibody [NB100-58842] - Differentiation of wt and Suv4-20dn teratomas. Representative images of Nanog staining in wt and Suv4-20dn teratomas. Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0025680) licensed under a CC-BY license.

Immunoprecipitation: Nanog Antibody [NB100-58842] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for IP at 6 ug per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody. For blotting immunoprecipitated Nanog, this was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells (NBA1-19349) with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Western Blot: Nanog Antibody [NB100-58842] - The forced expression of Tlk1 results in the aberrant downregulation of core pluripotency factors & attenuates self-renewal. (A) Immunoblot analysis of Oct4, Sox2, & Nanog levels in control mESCs (empty vector or doxycycline depletion) & Tlk1-overexpressing mESCs. The mESCs expressing an empty vector or the Tet-On-Tlk1 or Tet-On-Tlk1-D607A expression vector were cultured in the absence or presence of doxycycline (Dox; 100 ng/ml) for 24 hrs under undifferentiated self-renewal conditions. (B) Quantification of results from (A). The protein levels of the target genes were normalized to alpha -tubulin levels. The protein expression levels of each mESC line not treated with doxycycline were normalized to 1. The biological data are presented as mean (n = 6) ± SEM. *Р < 0.05, & **P < 0.01. (C) The morphology & AP staining of Tet-On-inducible Tlk1-expressing cell lines cultured in mock (Dox−) or doxycycline (Dox+) for 48 hrs. Scale bar, 500 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha -tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha -tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nanog Antibody [NB100-58842] - Tlk1 is not required for mESC self-renewal or pluripotency. (A) The efficiency of Tlk1 knockdown (KD) in control (shLuc) & Tlk1-KD mESCs (shTlk1 #1 & #2) was confirmed by RT-qPCR analysis. Data are mean (n = 3) ± SEM. **Р < 0.01 & ***Р < 0.001. (B) The morphology of control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs was evaluated using phase-contrast microscopic images & AP staining. Scale bars represent 500 µm. (C & D) The mRNA expression of pluripotency-associated & development-associated genes were analyzed by RT-qPCR in control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs cultured under undifferentiated self-renewal conditions. All data were normalized to Gapdh & plotted relative to the expression level in control cells. Data are means (n = 3) ± SEM. *Р < 0.05, **Р < 0.01, & ***Р < 0.001. (E) The protein levels of pluripotency factors in control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs was analyzed by immunoblotting using antibodies specific to Oct4, Sox2, & Nanog. (F) Quantification based on densitometry of Western blotting data from (E). All data were normalized to alpha -tubulin. Data are means (n = 3) ± SEM. *Р < 0.05, **Р < 0.01, & ***Р < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha -tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha -tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP, ChIP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Nanog Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to the mouse Nanog protein (within residues 1-50). [Swiss-Prot Q80Z64]
• Localization: Nucleus.
• Marker: Embryonic Stem Cell Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NANOG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:250-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Knockout Validated
  • Western Blot 1:5000-1:15000
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA (pH 9.0) is recommended. Use in Chromatin Immunoprecipitation and Flow Cytometry were reported in scientific publication (PMID: 23735977). IHC-P was reported in scientific publication (PMID: 25988972 ).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nanog Antibody - BSA Free

• FLJ12581
• FLJ40451
• hNanog
• homeobox protein NANOG
• Homeobox transcription factor Nanog
• homeobox transcription factor Nanog-delta 48
• Nanog homeobox
• Nanog

Background

In stem cell development, the influence of the transcription factor Nanog is important for appropriate differentiation, or conversely to maintain their pluripotent state. Islet survivor cells, generated for progenitor cell testing, have nuclear expression of OCT4, SOX2 and Nanog, indicating a probability that they can maintain pluripotent characteristics in experimentation. In brain cancer, there are some stem cell like behaviors, and the cancer cells exhibiting these characteristics demonstrate high concentrations of Nanog, influenced by p53. Nanog seems to be very important for stem cell growth, maintenance and differentiation, but is usually co-expressed and co-activated by other factors, indicating the need for further mechanistic exploration into its activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nanog Antibody (NB100-58842)(62)

Publications using NB100-58842

• Ma Z, Zhang F, Xiong J et al. Activation of embryonic/germ cell-like axis links poor outcomes of gliomas Cancer cell international 2022-11-26 [PMID: 36435765] (ICC/IF, Human)
• MESSAGGIO F. Characterization and Immunomodulatory Properties of Tissue and Cellular Products . Thesis (IHC-P, Human)
• Gao Y, Tan DS, Girbig M et Al. The emergence of Sox and POU transcription factors predates the origins of animal stem cells Nat Commun 2024-11-15 [PMID: 39543096]
• Shen YR, Zaballa S, Bech X, Sancho-Balsells A et Al. Expansion of the neocortex and protection from neurodegeneration by in vivo transient reprogramming Cell Stem Cell 2024-10-19 [PMID: 39426381]
• Kim B, Zhang S, Huang Y et Al. CRACD loss induces neuroendocrine cell plasticity of lung adenocarcinoma Cell Rep 2024-07-02 [PMID: 38796854]
• Chen R, Su F, Zhang T et Al. N6-methyladenosine modification of B7-H3 mRNA promotes the development and progression of colorectal cancer iScience 2024-01-18 [PMID: 38318386]
• Filidou E, Kandilogiannakis L, Tarapatzi G et al. A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood Biomedicines 2023-03-05 [PMID: 36979766] (Immunocytochemistry/ Immunofluorescence, Human)
• Ma Y, Sun W, Zhao L et al. Generation of an mESC model with a human hemophilia B nonsense mutation via CRISPR/Cas9 technology Stem cell research & therapy 2022-07-26 [PMID: 35883203] (ICC, Mouse)
• Zhou H, Sun H, Rong Z, Cui W Generation of Mt3 Homozygote murine ES cell lines via CRISPR/Cas9 technology Stem cell research 2022-02-18 [PMID: 35217495] (ICC/IF, Mouse)
• Yao M, Su P, Li Z et al. Knockout of Dip2c in murine ES cell line IBMSe001-B-1 by CRISPR/Cas9 genome editing technology Stem Cell Research 2021-02-01 [PMID: 33813174] (ICC, Human)
• Li W, Kou J, Qin J, et al. NADPH levels affect cellular epigenetic state by inhibiting HDAC3-Ncor complex Nature metabolism 2021-01-01 [PMID: 33462516]
• Baruah J, Chaudhuri S, Mastej V et al. Low-Level Nanog Expression in the Regulation of Quiescent Endothelium Arterioscler. Thromb. Vasc. Biol. 2020-07-09 [PMID: 32640900] (Chemotaxis, Human)
• Hayward BE, Steinbach PJ, Usdin K A point mutation in the nuclease domain of MLH3 eliminates repeat expansions in a mouse stem cell model of the Fragile X-related disorders Nucleic Acids Res. 2020-07-03 [PMID: 32619224] (ICC/IF, Mouse)
• Yao M, Yang Q, Lian M, Su P et Al. Generation of Dip2a homozygous knockout murine ES cell line IBMSe001-A-1 via CRISPR/Cas9 technology Stem Cell Res 2020-05-04 [PMID: 32361465]
• Sun H, Yang X, Liang L et al. Metabolic switch and epithelial-mesenchymal transition cooperate to regulate pluripotency EMBO J. 2020-04-15 [PMID: 32090361] (ICC/IF, Mouse)
• Liu Z, Yao M, Hu G, Yao H Generation of Rybp Homozygous Knockout murine ES cell line GIBHe001-A-1 by using CRISPR/Cas9 technology Stem Cell Research 2019-10-01 [PMID: 31794887] (FLOW, KO, ICC/IF, WB, Mouse)
• Castelli A, Susani L, Menale C et al. Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells Stem Cells 2019-03-20 [PMID: 30895693] (ICC/IF, Mouse)
• Glennon EKK, Austin LS, Arang N et al. Alterations in Phosphorylation of Hepatocyte Ribosomal Protein S6 Control Plasmodium Liver Stage Infection Cell Rep 2019-03-19 [PMID: 30893610] (FLOW, Human)
• Kodama Y, Harinath D, Mihara-Tomiyama N et al. Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression Biochem. Biophys. Res. Commun. 2019-02-19 [PMID: 30654938] (IF/IHC, Mouse)
• Ahmed MF, Kamel El-Sayed A, Chen H et al. Direct conversion of mouse embryonic fibroblast to osteoblast cells using hLMP-3 with Yamanaka factors. Int. J. Biochem. Cell Biol. 2018-11-16 [PMID: 30453092] (ICC/IF, Mouse)
• Liu C, Cai Z, Jin G et al. Abnormal gametogenesis induced by p53 deficiency promotes tumor progression and drug resistance. Cell Discov. 2018-10-02 [PMID: 30302273] (ICC/IF, Mouse)
• Veerapandian V, Ackermann JO, Srivastava Y et al. Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing Stem Cell Reports 2018-08-14 [PMID: 30078555] (ICC/IF, Mouse)
• Ma Z, Zhang F, Zhang H Et Al. Activation of Embryonic/germ Cell-like Axis Links Poor Outcomes of Gliomas Research Square 2021-07-26
• Srivastava, Y, Tan, D S Et al. Cancer-associated missense mutations enhance the pluripotency reprogramming activity of OCT4 and SOX17. FEBS J 2020-01-01 [PMID: 31569299] (IF/IHC, WB, Human)
• Malik V, Glaser LV, Zimmer D et al. Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2 Nat Commun 2019-08-02 [PMID: 31375664] (ICC/IF, Mouse)
• Lee J, Kim MS, Park SH, Jang YK. Tousled-like kinase 1 is a negative regulator of core transcription factors in murine embryonic stem cells Sci Rep 2018-01-10 [PMID: 29321513] (WB, ICC/IF, Mouse)
• Zhang N, Lyu Y, Pan X et al. miR-146b-5p promotes the neural conversion of pluripotent stem cells by targeting Smad4 Int. J. Mol. Med. 2017-07-11 [PMID: 28713933] (ICC/IF, Mouse)
• Ocampo A, Reddy P et al. In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming. Cell 2016-12-15 [PMID: 27984723] (IF/IHC, Mouse)
• Pandolfini L, Luzi E, Bressan D et al. RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells. Genome Biol. 2016-05-07 [PMID: 27154007] (WB, ICC/IF, Mouse)
• Paulis M, Castelli A, Susani L et al. Chromosome transplantation as a novel approach for correcting complex genomic disorders. Oncotarget 2015-11-03 [PMID: 26485770] (ICC/IF)
• Neri T, Muggeo S, Paulis M et al. Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts. Stem Cell Reports 2015-09-02 [PMID: 26344905] (ICC/IF, Mouse)
• Kim J, Liu Y, Qiu M, Xu Y. Pluripotency factor Nanog is tumorigenic by deregulating DNA damage response in somatic cells Oncogene. 2015-06-15 [PMID: 26073077] (IF/IHC, WB, Mouse)
• Palla AR, Piazzolla D, Alcazar N et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep 2015-01-01 [PMID: 25988972] (IHC-P, Mouse)
• Bertacchi M, Pandolfini L, D'Onofrio M et al. The double inhibition of endogenously-produced BMP and Wnt factors synergistically triggers dorsal telencephalic differentiation of mouse ES cells. Dev Neurobiol 2014-07-15 [PMID: 25044881] (ICC/IF, Mouse)
  
  Details:
  Nanog antibody used for ICC-IF on undifferentiated mouse ES cells (E14 Tg2A ES cell line) alongwith another pluripotency marker (Oct4) at Day 0 and 2 of in-vitro differentiation (Supporting Information Fig. 1A) - Cells grown on Poly-ornithine/Laminin coated glass coverslips, fixed with 2% paraformaldehyde for 10-15 min, permeabilized using 0.1% Triton X100 in PBS and blocked with 0.5% BSA in PBS, primary used at 1:300 dilution - incubated 2 h RT.
• Piazzolla D, Palla A R et al. Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia. Nat Commun 2014-01-07 [PMID: 24979572] (WB, IF/IHC, Chemotaxis, Mouse)
• Carelli S, Messaggio F, Canazza A et al. Characteristics and Properties of Mesenchymal Stem Cells Derived from Micro-fragmented Adipose Tissue. Cell Transplant. 2014-05-06 [PMID: 24806078] (IHC-P, Human)
• Vaz MA, Martinez JC, Devesa JM et al. Prognostic Value of Stem Cell Quantification in Stage II Colon Cancer. PLoS ONE 2014-03-03 [PMID: 24586330] (IHC-P, Human)
• Abad M, Mosteiro L, Pantoja C et al. Reprogramming in vivo produces teratomas and iPS cells with totipotency features. Nature. 2013-10-17 [PMID: 24025773] (ICC/IF, Mouse)
• Schneider RP, Garrobo I, Foronda M et al. TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells. Nat Commun 2013-06-05 [PMID: 23735977] (FLOW, Chemotaxis, Mouse)
• Bertacchi M, Pandolfini L, Murenu E et al. The positional identity of mouse ES cell-generated neurons is affected by BMP signaling Cell Mol Life Sci 2012-10-16 [PMID: 23069989] (ICC/IF, Mouse)
• Liu SP, Harn HJ, Chien YJ et al. n-Butylidenephthalide (BP) Maintains Stem Cell Pluripotency by Activating Jak2/Stat3 Pathway and Increases the Efficiency of iPS Cells Generation PLoS One 2012-01-01 [PMID: 22970157] (ICC/IF, Mouse)
• Deshpande AM, Khalid O, Kim JJ et al. Cdk2ap2 is a novel regulator for self-renewal of murine embryonic stem cells. Stem Cells Dev 2012-11-01 [PMID: 22548356]
• Camnasio S, Carri AD, Lombardo A, Grad I, Mariotti C, Castucci A, Rozell B, Riso PL, Castiglioni V, Zuccato C, Rochon C, Takashima Y, Diaferia G, Biunno I, Gellera C, Jaconi M, Smith A, Hovatta O, Naldini L, Di Donato S, Feki A, Cattaneo E. The first reported generation of several induced pluripotent stem cell lines from homozygous and heterozygous Huntington's disease patients demonstrates mutation related enhanced lysosomal activity. Neurobiol Dis;46(1):41-51. 2012-04-01 [PMID: 22405424] (ICC/IF, Human)
• Castiglioni V, Onorati M, Rochon C, Cattaneo E. Induced pluripotent stem cell lines from Huntington’s disease mice undergo neuronal differentiation while showing alterations in the lysosomal pathway. Neurobiology of Disease. 2011-12-28 [PMID: 22227000]
• Marion RM, Schotta G, Ortega S, Blasco MA. Suv4-20h Abrogation Enhances Telomere Elongation during Reprogramming and Confers a Higher Tumorigenic Potential to iPS Cells. PLoS ONE;6(10). 2011-01-01 [PMID: 22022429] (IF/IHC, Mouse)
• Villasante A, Piazzolla D, Li H et al. Epigenetic regulation of Nanog expression by Ezh2 in pluripotent stem cells. Cell Cycle 2011-05-01 [PMID: 21490431] (ICC/IF, Mouse)
• Abdelalim EM, Tooyama I. NPR-A regulates self-renewal and pluripotency of embryonic stem cells. Cell Death Dis 2011-03-01 [PMID: 21390061]
• Tejedo JR, Tapia-Limonchi R, Mora-Castilla S et al. Low concentrations of nitric oxide delay the differentiation of embryonic stem cells and promote their survival. Cell Death Dis 2010-10-01 [PMID: 21368853]
• Kohler EE, Cowan CE, Chatterjee I et al. NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis. Blood 2010-11-30 [PMID: 21119109]
• Shin J, Bossenz M, Chung Y et al. Maternal Rnf12/RLIM is required for imprinted X-chromosome inactivation in mice. Nature 2010-10-01 [PMID: 20962847]
• Moretto-Zita M, Jin H, Shen Z et al. Phosphorylation stabilizes Nanog by promoting its interaction with Pin1. Proc Natl Acad Sci U S A 2010-07-01 [PMID: 20622153]
• Hanna J, Saha K, Pando B et al. Direct cell reprogramming is a stochastic process amenable to acceleration. Nature 2009-12-01 [PMID: 19898493]
• Schaniel C, Ang YS, Ratnakumar K et al. Smarcc1/Baf155 couples self-renewal gene repression with changes in chromatin structure in mouse embryonic stem cells. Stem Cells 2009-12-01 [PMID: 19785031] (WB, Mouse)
• Madan B, Madan V, Weber O et al. The pluripotency-associated gene Dppa4 is dispensable for embryonic stem cell identity and germ cell development but essential for embryogenesis. Mol Cell Biol 2009-06-01 [PMID: 19332562]
• Izadyar F, Pau F, Marh J et al. Generation of multipotent cell lines from a distinct population of male germ line stem cells. Reproduction 2008-06-01 [PMID: 18502893]
• Liang J, Wan M, Zhang Y et al. Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells. Nat Cell Biol 2008-06-01 [PMID: 18454139]
• Wernig M, Zhao JP, Pruszak J et al. Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease. Proc Natl Acad Sci U S A 2008-04-01 [PMID: 18391196]
• Cole MF, Johnstone SE, Newman JJ et al. Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells. Genes Dev 2008-03-01 [PMID: 18347094]
• Quinlan MP, Quatela SE, Philips MR et al. Activated Kras, but not Hras or Nras, may initiate tumors of endodermal origin via stem cell expansion. Mol Cell Biol 2008-04-01 [PMID: 18268007]
• Meissner A, Wernig M, Jaenisch R et al. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nat Biotechnol 2007-10-01 [PMID: 17724450]
• Wernig M, Meissner A, Foreman R et al. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 2007-07-01 [PMID: 17554336]
• Hough SR, Clements I, Welch PJ et al. Differentiation of mouse embryonic stem cells after RNA interference-mediated silencing of OCT4 and Nanog. Stem Cells 2006-06-01 [PMID: 16456133]

Bioinformatics

• Gene Symbol: NANOG
• Entrez:
  • 71950(Mouse)
• Uniprot:
  • Q80Z64()